Restriction map for
pF1KB8730
(Linear) (Six-base) MAPSORT of: KIBB8730.seq Check: 2359 from: 1 to: 1179
KIBB8730.orf
With 182 enzymes: SgfI *
April 2, 2009 17:32 ..
AccI GT'mk_AC
Cuts at: 0 1126 1179
Size: 1126 53
AclI AA'CG_TT
Cuts at: 0 89 1179
Size: 89 1090
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 973 1126 1179
Size: 185 788 153 53
Fragments arranged by size:
788 185 153 53
AfeI AGC'GCT
Cuts at: 0 541 1009 1179
Size: 541 468 170
Fragments arranged by size:
541 468 170
AflIII A'CryG_T
Cuts at: 0 707 1179
Size: 707 472
AlwNI CAG_nnn'CTG
Cuts at: 0 205 722 1179
Size: 205 517 457
Fragments arranged by size:
517 457 205
ApoI r'AATT_y
Cuts at: 0 597 1179
Size: 597 582
BanI G'GyrC_C
Cuts at: 0 460 651 1179
Size: 460 191 528
Fragments arranged by size:
528 460 191
BanII G_rGCy'C
Cuts at: 0 35 1092 1179
Size: 35 1057 87
Fragments arranged by size:
1057 87 35
BbsI GAAGACnn'nnnn_
Cuts at: 0 173 779 1179
Size: 173 606 400
Fragments arranged by size:
606 400 173
BbvCI CC'TCA_GC
Cuts at: 0 572 1179
Size: 572 607
Bme1580I G_kGCm'C
Cuts at: 0 656 1179
Size: 656 523
BmrI ACTGGGnnnn_n'
Cuts at: 0 802 1179
Size: 802 377
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 561 693 1106 1179
Size: 561 132 413 73
Fragments arranged by size:
561 413 132 73
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 762 1080 1179
Size: 762 318 99
Fragments arranged by size:
762 318 99
Bpu10I CC'TnA_GC
Cuts at: 0 572 1179
Size: 572 607
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1033 1179
Size: 1033 146
BsaI GGTCTCn'nnnn_
Cuts at: 0 833 1179
Size: 833 346
BsaAI yAC'GTr
Cuts at: 0 374 1179
Size: 374 805
BsaWI w'CCGG_w
Cuts at: 0 740 1179
Size: 740 439
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 28 922 952 1179
Size: 28 894 30 227
Fragments arranged by size:
894 227 30 28
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 560 930 1179
Size: 19 541 370 249
Fragments arranged by size:
541 370 249 19
BseYI C'CCAG_C
Cuts at: 0 947 1063 1179
Size: 947 116 116
Fragments arranged by size:
947 116 116
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 209 239 448 1179
Size: 209 30 209 731
Fragments arranged by size:
731 209 209 30
BsiHKAI G_wGCw'C
Cuts at: 0 910 921 1179
Size: 910 11 258
Fragments arranged by size:
910 258 11
BsmI GAATG_Cn'
Cuts at: 0 200 342 1179
Size: 200 142 837
Fragments arranged by size:
837 200 142
Bsp1286I G_dGCh'C
Cuts at: 0 35 656 910 921 1092 1179
Size: 35 621 254 11 171 87
Fragments arranged by size:
621 254 171 87 35 11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 427 1179
Size: 427 752
BsrDI GCAATG_nn'
Cuts at: 0 122 1179
Size: 122 1057
BsrFI r'CCGG_y
Cuts at: 0 243 842 1179
Size: 243 599 337
Fragments arranged by size:
599 337 243
BssSI C'ACGA_G
Cuts at: 0 889 1179
Size: 889 290
BstYI r'GATC_y
Cuts at: 0 18 992 1179
Size: 18 974 187
Fragments arranged by size:
974 187 18
Bsu36I CC'TnA_GG
Cuts at: 0 668 1179
Size: 668 511
BtgI C'CryG_G
Cuts at: 0 476 996 1179
Size: 476 520 183
Fragments arranged by size:
520 476 183
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 569 1179
Size: 569 610
BtsI GCAGTG_nn'
Cuts at: 0 919 1179
Size: 919 260
DrdI GACnn_nn'nnGTC
Cuts at: 0 357 1179
Size: 357 822
EaeI y'GGCC_r
Cuts at: 0 410 479 1179
Size: 410 69 700
Fragments arranged by size:
700 410 69
EarI CTCTTCn'nnn_
Cuts at: 0 851 1179
Size: 851 328
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 762 973 1080 1126 1179
Size: 185 577 211 107 46 53
Fragments arranged by size:
577 211 185 107 53 46
EcoO109I rG'GnC_Cy
Cuts at: 0 1165 1179
Size: 1165 14
HaeII r_GCGC'y
Cuts at: 0 525 543 1011 1179
Size: 525 18 468 168
Fragments arranged by size:
525 468 168 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 30 1179
Size: 30 1149
MscI TGG'CCA
Cuts at: 0 412 481 1179
Size: 412 69 698
Fragments arranged by size:
698 412 69
MspA1I CmG'CkG
Cuts at: 0 432 1179
Size: 432 747
NcoI C'CATG_G
Cuts at: 0 476 1179
Size: 476 703
NspI r_CATG'y
Cuts at: 0 711 1179
Size: 711 468
PasI CC'CwG_GG
Cuts at: 0 901 1179
Size: 901 278
PciI A'CATG_T
Cuts at: 0 707 1179
Size: 707 472
PflMI CCAn_nnn'nTGG
Cuts at: 0 117 970 1179
Size: 117 853 209
Fragments arranged by size:
853 209 117
PshAI GACnn'nnGTC
Cuts at: 0 173 1179
Size: 173 1006
PvuII CAG'CTG
Cuts at: 0 432 1179
Size: 432 747
SgrAI Cr'CCGG_yG
Cuts at: 0 243 1179
Size: 243 936
SmlI C'TyrA_G
Cuts at: 0 1048 1179
Size: 1048 131
SspI AAT'ATT
Cuts at: 0 979 1179
Size: 979 200
StuI AGG'CCT
Cuts at: 0 1039 1179
Size: 1039 140
StyI C'CwwG_G
Cuts at: 0 476 1040 1179
Size: 476 564 139
Fragments arranged by size:
564 476 139
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1093 1125 1179
Size: 1093 32 54
Fragments arranged by size:
1093 54 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 266 765 1179
Size: 266 499 414
Fragments arranged by size:
499 414 266
Enzymes that do cut:
AccI AclI AcuI AfeI AflIII AlwNI ApoI BanI
BanII BbsI BbvCI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BssSI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI
EcoO109I HaeII Hin4I MscI MspA1I NcoI NspI PasI
PciI PflMI PshAI PvuII SgrAI SmlI SspI StuI
StyI TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AflII AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BlpI BmgBI BmtI BsaBI BsaHI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MslI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PfoI PmeI PmlI PpiI PpuMI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfcI
SfiI SfoI SgfI SmaI SnaBI SpeI SphI SrfI
SwaI TaqII TatI Tth111I XbaI XhoI XmaI XmnI
ZraI