(Linear) (Six-base) MAPSORT of: KIBB8731.seq Check: 3745 from: 1 to: 2058 KIBB8731.orf With 182 enzymes: SgfI * March 3, 2008 11:07 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 651 819 1239 1491 2058 Size: 344 307 168 420 252 567 Fragments arranged by size: 567 420 344 307 252 168 AflIII A'CryG_T Cuts at: 0 1113 2058 Size: 1113 945 AleI CACnn'nnGTG Cuts at: 0 1214 1718 2058 Size: 1214 504 340 Fragments arranged by size: 1214 504 340 ApaLI G'TGCA_C Cuts at: 0 1783 2058 Size: 1783 275 ApoI r'AATT_y Cuts at: 0 304 352 617 701 785 869 1121 1289 Size: 304 48 265 84 84 84 252 168 Cuts at: 1289 1373 1457 2045 2058 Size: 84 84 588 13 Fragments arranged by size: 588 304 265 252 168 84 84 84 84 84 48 13 BamHI G'GATC_C Cuts at: 0 75 2058 Size: 75 1983 BclI T'GATC_A Cuts at: 0 858 2058 Size: 858 1200 BfrBI ATG'CAT Cuts at: 0 811 979 2058 Size: 811 168 1079 Fragments arranged by size: 1079 811 168 BlpI GC'TnA_GC Cuts at: 0 1182 2058 Size: 1182 876 Bme1580I G_kGCm'C Cuts at: 0 1787 2058 Size: 1787 271 BmrI ACTGGGnnnn_n' Cuts at: 0 971 1391 2058 Size: 971 420 667 Fragments arranged by size: 971 667 420 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 647 815 899 1487 1571 2058 Size: 647 168 84 588 84 487 Fragments arranged by size: 647 588 487 168 84 84 BsaBI GATnn'nnATC Cuts at: 0 260 2058 Size: 260 1798 BsaWI w'CCGG_w Cuts at: 0 98 2058 Size: 98 1960 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 73 2058 Size: 73 1985 BsiHKAI G_wGCw'C Cuts at: 0 1687 1787 2058 Size: 1687 100 271 Fragments arranged by size: 1687 271 100 BsmI GAATG_Cn' Cuts at: 0 916 1246 1489 2058 Size: 916 330 243 569 Fragments arranged by size: 916 569 330 243 Bsp1286I G_dGCh'C Cuts at: 0 1687 1787 2058 Size: 1687 100 271 Fragments arranged by size: 1687 271 100 BspHI T'CATG_A Cuts at: 0 861 1449 2058 Size: 861 588 609 Fragments arranged by size: 861 609 588 BsrDI GCAATG_nn' Cuts at: 0 342 938 1442 1494 2058 Size: 342 596 504 52 564 Fragments arranged by size: 596 564 504 342 52 BsrGI T'GTAC_A Cuts at: 0 1194 2058 Size: 1194 864 BstAPI GCAn_nnn'nTGC Cuts at: 0 1021 2058 Size: 1021 1037 BstYI r'GATC_y Cuts at: 0 75 2058 Size: 75 1983 BtgI C'CryG_G Cuts at: 0 196 2058 Size: 196 1862 BtsI GCAGTG_nn' Cuts at: 0 1687 2058 Size: 1687 371 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 1476 1511 2058 Size: 1476 35 547 Fragments arranged by size: 1476 547 35 EarI CTCTTCn'nnn_ Cuts at: 0 61 1031 2058 Size: 61 970 1027 Fragments arranged by size: 1027 970 61 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 344 647 651 815 819 899 1239 1487 Size: 344 303 4 164 4 80 340 248 Cuts at: 1487 1491 1571 2058 Size: 4 80 487 Fragments arranged by size: 487 344 340 303 248 164 80 80 4 4 4 EcoRI G'AATT_C Cuts at: 0 617 785 1121 1373 2045 2058 Size: 617 168 336 252 672 13 Fragments arranged by size: 672 617 336 252 168 13 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1287 1539 2058 Size: 1287 252 519 Fragments arranged by size: 1287 519 252 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 60 92 389 421 1338 1370 2058 Size: 60 32 297 32 917 32 688 Fragments arranged by size: 917 688 297 60 32 32 32 HindIII A'AGCT_T Cuts at: 0 746 1334 2058 Size: 746 588 724 Fragments arranged by size: 746 724 588 MslI CAynn'nnrTG Cuts at: 0 461 757 1130 1214 1718 1802 1886 1970 Size: 461 296 373 84 504 84 84 84 Cuts at: 1970 2058 Size: 88 Fragments arranged by size: 504 461 373 296 88 84 84 84 84 NcoI C'CATG_G Cuts at: 0 196 2058 Size: 196 1862 NdeI CA'TA_TG Cuts at: 0 519 1031 1199 1703 1955 2058 Size: 519 512 168 504 252 103 Fragments arranged by size: 519 512 504 252 168 103 NsiI A_TGCA'T Cuts at: 0 813 981 2058 Size: 813 168 1077 Fragments arranged by size: 1077 813 168 NspI r_CATG'y Cuts at: 0 815 983 1117 2058 Size: 815 168 134 941 Fragments arranged by size: 941 815 168 134 PasI CC'CwG_GG Cuts at: 0 12 2058 Size: 12 2046 PciI A'CATG_T Cuts at: 0 1113 2058 Size: 1113 945 PsiI TTA'TAA Cuts at: 0 698 1061 2058 Size: 698 363 997 Fragments arranged by size: 997 698 363 SapI GCTCTTCn'nnn_ Cuts at: 0 1031 2058 Size: 1031 1027 SfcI C'TryA_G Cuts at: 0 654 1905 2058 Size: 654 1251 153 Fragments arranged by size: 1251 654 153 StyI C'CwwG_G Cuts at: 0 196 2058 Size: 196 1862 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 189 2058 Size: 189 1869 TatI w'GTAC_w Cuts at: 0 1194 2058 Size: 1194 864 Tth111I GACn'n_nGTC Cuts at: 0 6 2058 Size: 6 2052 XmnI GAAnn'nnTTC Cuts at: 0 832 869 1270 1457 1756 1897 2058 Size: 832 37 401 187 299 141 161 Fragments arranged by size: 832 401 299 187 161 141 37 Enzymes that do cut: AcuI AflIII AleI ApaLI ApoI BamHI BclI BfrBI BlpI Bme1580I BmrI BpmI BsaBI BsaWI BseRI BsiHKAI BsmI Bsp1286I BspHI BsrDI BsrGI BstAPI BstYI BtgI BtsI CspCI EarI Eco57MI EcoRI FalI Hin4I HindIII MslI NcoI NdeI NsiI NspI PasI PciI PsiI SapI SfcI StyI TaqII TatI Tth111I XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HincII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NgoMIV NheI NotI NruI PacI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI XbaI XcmI XhoI XmaI ZraI