Restriction map for
pF1KB8736
(Linear) (Six-base) MAPSORT of: KIBB8736.seq Check: 6429 from: 1 to: 1200
KIBB8736.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 789 957 1041 1200
Size: 84 705 168 84 159
Fragments arranged by size:
705 168 159 84 84
AlwNI CAG_nnn'CTG
Cuts at: 0 707 1200
Size: 707 493
ApoI r'AATT_y
Cuts at: 0 25 263 550 1200
Size: 25 238 287 650
Fragments arranged by size:
650 287 238 25
BanI G'GyrC_C
Cuts at: 0 659 1200
Size: 659 541
BanII G_rGCy'C
Cuts at: 0 176 210 985 1200
Size: 176 34 775 215
Fragments arranged by size:
775 215 176 34
BbeI G_GCGC'C
Cuts at: 0 663 1200
Size: 663 537
BciVI GTATCCnnnnn_n'
Cuts at: 0 141 1200
Size: 141 1059
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 836 1088 1200
Size: 836 252 112
Fragments arranged by size:
836 252 112
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 701 785 869 953 1037 1121 1200
Size: 112 589 84 84 84 84 84 79
Fragments arranged by size:
589 112 84 84 84 84 84 79
Bpu10I CC'TnA_GC
Cuts at: 0 439 477 1200
Size: 439 38 723
Fragments arranged by size:
723 439 38
BsaHI Gr'CG_yC
Cuts at: 0 660 1200
Size: 660 540
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 814 1200
Size: 814 386
BsiHKAI G_wGCw'C
Cuts at: 0 985 1200
Size: 985 215
BsmI GAATG_Cn'
Cuts at: 0 512 703 787 955 1200
Size: 512 191 84 168 245
Fragments arranged by size:
512 245 191 168 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 824 1200
Size: 824 376
Bsp1286I G_dGCh'C
Cuts at: 0 176 210 985 1200
Size: 176 34 775 215
Fragments arranged by size:
775 215 176 34
BstYI r'GATC_y
Cuts at: 0 529 1200
Size: 529 671
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 684 1200
Size: 684 516
BtsI GCAGTG_nn'
Cuts at: 0 708 792 1200
Size: 708 84 408
Fragments arranged by size:
708 408 84
EaeI y'GGCC_r
Cuts at: 0 110 360 1200
Size: 110 250 840
Fragments arranged by size:
840 250 110
EarI CTCTTCn'nnn_
Cuts at: 0 217 708 1200
Size: 217 491 492
Fragments arranged by size:
492 491 217
EcoICRI GAG'CTC
Cuts at: 0 983 1200
Size: 983 217
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 84 112 701 785 789 869 953 957
Size: 84 28 589 84 4 80 84 4
Cuts at: 957 1037 1041 1121 1200
Size: 80 4 80 79
Fragments arranged by size:
589 84 84 84 80 80 80 79
28 4 4 4
EcoO109I rG'GnC_Cy
Cuts at: 0 69 1136 1200
Size: 69 1067 64
Fragments arranged by size:
1067 69 64
EcoRI G'AATT_C
Cuts at: 0 25 550 1200
Size: 25 525 650
Fragments arranged by size:
650 525 25
HaeII r_GCGC'y
Cuts at: 0 663 1200
Size: 663 537
HincII GTy'rAC
Cuts at: 0 413 501 1200
Size: 413 88 699
Fragments arranged by size:
699 413 88
HindIII A'AGCT_T
Cuts at: 0 474 1200
Size: 474 726
HpaI GTT'AAC
Cuts at: 0 501 1200
Size: 501 699
KasI G'GCGC_C
Cuts at: 0 659 1200
Size: 659 541
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 137 1200
Size: 137 1063
MscI TGG'CCA
Cuts at: 0 112 362 1200
Size: 112 250 838
Fragments arranged by size:
838 250 112
MslI CAynn'nnrTG
Cuts at: 0 517 1200
Size: 517 683
MspA1I CmG'CkG
Cuts at: 0 51 1200
Size: 51 1149
NarI GG'CG_CC
Cuts at: 0 660 1200
Size: 660 540
NdeI CA'TA_TG
Cuts at: 0 141 1200
Size: 141 1059
PpuMI rG'GwC_Cy
Cuts at: 0 69 1200
Size: 69 1131
PsiI TTA'TAA
Cuts at: 0 452 536 1200
Size: 452 84 664
Fragments arranged by size:
664 452 84
PstI C_TGCA'G
Cuts at: 0 326 1200
Size: 326 874
PvuII CAG'CTG
Cuts at: 0 51 1200
Size: 51 1149
SacI G_AGCT'C
Cuts at: 0 985 1200
Size: 985 215
SapI GCTCTTCn'nnn_
Cuts at: 0 708 1200
Size: 708 492
SfcI C'TryA_G
Cuts at: 0 75 322 834 1200
Size: 75 247 512 366
Fragments arranged by size:
512 366 247 75
SfoI GGC'GCC
Cuts at: 0 661 1200
Size: 661 539
StyI C'CwwG_G
Cuts at: 0 239 1200
Size: 239 961
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 608 1200
Size: 608 592
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 886 918 1200
Size: 886 32 282
Fragments arranged by size:
886 282 32
Enzymes that do cut:
AcuI AlwNI ApoI BanI BanII BbeI BciVI BplI
BpmI Bpu10I BsaHI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BstYI BtgZI BtsI EaeI EarI EcoICRI Eco57MI EcoO109I
EcoRI HaeII HincII HindIII HpaI KasI MmeI MscI
MslI MspA1I NarI NdeI PpuMI PsiI PstI PvuII
SacI SapI SfcI SfoI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI
BcgI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI
BseRI BseYI BsiEI BsiWI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRV FalI FseI FspI
FspAI Hin4I KpnI MfeI MluI NaeI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TatI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI