(Linear) (Six-base) MAPSORT of: KIBB8738.seq  Check: 2777  from: 1  to: 333



KIBB8738.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:07  ..

ApoI r'AATT_y

Cuts at:      0     316     333 
   Size:        316      17
 
BanII G_rGCy'C

Cuts at:      0     290     333 
   Size:        290      43
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      65     333 
   Size:         65     268
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     283     333 
   Size:        283      50
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     238     268     333 
   Size:        238      30      65

  Fragments arranged by size: 

                 238      65      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      89     229     333 
   Size:         89     140     104

  Fragments arranged by size: 

                 140     104      89
 
BsiHKAI G_wGCw'C

Cuts at:      0     290     333 
   Size:        290      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     290     333 
   Size:        290      43
 
BspHI T'CATG_A

Cuts at:      0     309     333 
   Size:        309      24
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      42     333 
   Size:         42     291
 
EarI CTCTTCn'nnn_

Cuts at:      0      20     333 
   Size:         20     313
 
EcoICRI GAG'CTC

Cuts at:      0     288     333 
   Size:        288      45
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      71     333 
   Size:         71     262
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     249     281     333 
   Size:        249      32      52

  Fragments arranged by size: 

                 249      52      32
 
MslI CAynn'nnrTG

Cuts at:      0     311     333 
   Size:        311      22
 
PstI C_TGCA'G

Cuts at:      0      99     254     333 
   Size:         99     155      79

  Fragments arranged by size: 

                 155      99      79
 
SacI G_AGCT'C

Cuts at:      0     290     333 
   Size:        290      43
 
SbfI CC_TGCA'GG

Cuts at:      0      99     333 
   Size:         99     234
 
SfcI C'TryA_G

Cuts at:      0      95     250     333 
   Size:         95     155      83

  Fragments arranged by size: 

                 155      95      83
 
SspI AAT'ATT

Cuts at:      0       9     206     333 
   Size:          9     197     127

  Fragments arranged by size: 

                 197     127       9
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     169     333 
   Size:        169     164
 

 Enzymes that do cut:

     ApoI    BanII     BbsI    BciVI    BsaXI    BseRI  BsiHKAI Bsp1286I
    BspHI   BstAPI     EarI  EcoICRI    EcoNI    Hin4I     MslI     PstI
     SacI     SbfI     SfcI     SspI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EciI  Eco57MI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII    SacII     SalI    SanDI     SapI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI     TatI     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI