(Linear) (Six-base) MAPSORT of: KIBB8739.seq Check: 4396 from: 1 to: 156 KIBB8739.orf With 182 enzymes: SgfI * March 3, 2008 11:07 .. BlpI GC'TnA_GC Cuts at: 0 27 156 Size: 27 129 BmgBI CAC'GTC Cuts at: 0 73 156 Size: 73 83 BsaBI GATnn'nnATC Cuts at: 0 136 156 Size: 136 20 EarI CTCTTCn'nnn_ Cuts at: 0 33 156 Size: 33 123 MslI CAynn'nnrTG Cuts at: 0 55 145 156 Size: 55 90 11 Fragments arranged by size: 90 55 11 SapI GCTCTTCn'nnn_ Cuts at: 0 33 156 Size: 33 123 SfcI C'TryA_G Cuts at: 0 82 156 Size: 82 74 Enzymes that do cut: BlpI BmgBI BsaBI EarI MslI SapI SfcI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI