Restriction map for
pF1KB8740
(Linear) (Six-base) MAPSORT of: KIBB8740.seq Check: 7964 from: 1 to: 549
KIBB8740.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 458 549
Size: 458 91
AflIII A'CryG_T
Cuts at: 0 257 549
Size: 257 292
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 421 422 453 454 549
Size: 421 1 31 1 95
Fragments arranged by size:
421 95 31 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 359 549
Size: 359 190
AvaI C'yCGr_G
Cuts at: 0 136 216 251 549
Size: 136 80 35 298
Fragments arranged by size:
298 136 80 35
BanI G'GyrC_C
Cuts at: 0 67 99 549
Size: 67 32 450
Fragments arranged by size:
450 67 32
BbeI G_GCGC'C
Cuts at: 0 71 549
Size: 71 478
BbsI GAAGACnn'nnnn_
Cuts at: 0 428 549
Size: 428 121
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 222 256 282 316 549
Size: 222 34 26 34 233
Fragments arranged by size:
233 222 34 34 26
Bme1580I G_kGCm'C
Cuts at: 0 104 125 549
Size: 104 21 424
Fragments arranged by size:
424 104 21
BmgBI CAC'GTC
Cuts at: 0 120 549
Size: 120 429
BmrI ACTGGGnnnn_n'
Cuts at: 0 199 549
Size: 199 350
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 549
Size: 208 341
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 549
Size: 265 284
BsaAI yAC'GTr
Cuts at: 0 273 549
Size: 273 276
BsaBI GATnn'nnATC
Cuts at: 0 7 549
Size: 7 542
BsaHI Gr'CG_yC
Cuts at: 0 68 410 549
Size: 68 342 139
Fragments arranged by size:
342 139 68
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 205 361 549
Size: 205 156 188
Fragments arranged by size:
205 188 156
BseYI C'CCAG_C
Cuts at: 0 103 337 549
Size: 103 234 212
Fragments arranged by size:
234 212 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 445 549
Size: 301 144 104
Fragments arranged by size:
301 144 104
BsiHKAI G_wGCw'C
Cuts at: 0 473 549
Size: 473 76
Bsp1286I G_dGCh'C
Cuts at: 0 104 125 473 549
Size: 104 21 348 76
Fragments arranged by size:
348 104 76 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 458 549
Size: 458 91
BsrFI r'CCGG_y
Cuts at: 0 333 412 549
Size: 333 79 137
Fragments arranged by size:
333 137 79
BsrGI T'GTAC_A
Cuts at: 0 260 549
Size: 260 289
BstAPI GCAn_nnn'nTGC
Cuts at: 0 468 549
Size: 468 81
BtsI GCAGTG_nn'
Cuts at: 0 180 360 549
Size: 180 180 189
Fragments arranged by size:
189 180 180
DraIII CAC_nnn'GTG
Cuts at: 0 273 468 549
Size: 273 195 81
Fragments arranged by size:
273 195 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 208 549
Size: 208 341
EcoO109I rG'GnC_Cy
Cuts at: 0 312 549
Size: 312 237
HaeII r_GCGC'y
Cuts at: 0 71 227 549
Size: 71 156 322
Fragments arranged by size:
322 156 71
KasI G'GCGC_C
Cuts at: 0 67 549
Size: 67 482
MfeI C'AATT_G
Cuts at: 0 537 549
Size: 537 12
MslI CAynn'nnrTG
Cuts at: 0 379 549
Size: 379 170
NaeI GCC'GGC
Cuts at: 0 414 549
Size: 414 135
NarI GG'CG_CC
Cuts at: 0 68 549
Size: 68 481
NgoMIV G'CCGG_C
Cuts at: 0 412 549
Size: 412 137
NspI r_CATG'y
Cuts at: 0 261 549
Size: 261 288
PciI A'CATG_T
Cuts at: 0 257 549
Size: 257 292
PflMI CCAn_nnn'nTGG
Cuts at: 0 337 549
Size: 337 212
PpuMI rG'GwC_Cy
Cuts at: 0 312 549
Size: 312 237
SfoI GGC'GCC
Cuts at: 0 69 549
Size: 69 480
SgrAI Cr'CCGG_yG
Cuts at: 0 412 549
Size: 412 137
SmaI CCC'GGG
Cuts at: 0 138 549
Size: 138 411
SmlI C'TyrA_G
Cuts at: 0 280 549
Size: 280 269
StyI C'CwwG_G
Cuts at: 0 368 549
Size: 368 181
TatI w'GTAC_w
Cuts at: 0 260 549
Size: 260 289
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 375 549
Size: 375 174
XmaI C'CCGG_G
Cuts at: 0 136 549
Size: 136 413
XmnI GAAnn'nnTTC
Cuts at: 0 58 549
Size: 58 491
Enzymes that do cut:
AarI AflIII AloI AlwNI AvaI BanI BbeI BbsI
BcgI Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaBI
BsaHI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI
BsrGI BstAPI BtsI DraIII Eco57MI EcoO109I HaeII KasI
MfeI MslI NaeI NarI NgoMIV NspI PciI PflMI
PpuMI SfoI SgrAI SmaI SmlI StyI TatI XcmI
XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AleI AlfI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbvCI BciVI BclI
BfrBI BglI BglII BlpI BmtI BplI Bpu10I BsaI
BsaWI BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspI FspAI Hin4I HincII HindIII HpaI
KpnI MluI MmeI MscI MspA1I NcoI NdeI NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfcI SfiI SgfI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI ZraI