(Linear) (Six-base) MAPSORT of: KIBB8741.seq  Check: 5286  from: 1  to: 417



KIBB8741.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     417 
   Size:        347      70
 
AlwNI CAG_nnn'CTG

Cuts at:      0     160     417 
   Size:        160     257
 
ApoI r'AATT_y

Cuts at:      0      96     198     222     289     417 
   Size:         96     102      24      67     128

  Fragments arranged by size: 

                 128     102      96      67      24
 
BanI G'GyrC_C

Cuts at:      0     283     417 
   Size:        283     134
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      88     417 
   Size:         88     329
 
BglI GCCn_nnn'nGGC

Cuts at:      0     169     417 
   Size:        169     248
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     348     417 
   Size:        348      69
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     245     417 
   Size:        245     172
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     174     417 
   Size:        174     243
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     338     417 
   Size:        338      79
 
DraI TTT'AAA

Cuts at:      0     255     417 
   Size:        255     162
 
DraIII CAC_nnn'GTG

Cuts at:      0     313     417 
   Size:        313     104
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     347     417 
   Size:        347      70
 
EcoO109I rG'GnC_Cy

Cuts at:      0     238     342     417 
   Size:        238     104      75

  Fragments arranged by size: 

                 238     104      75
 
EcoRI G'AATT_C

Cuts at:      0      96     222     417 
   Size:         96     126     195

  Fragments arranged by size: 

                 195     126      96
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     289     321     417 
   Size:        289      32      96

  Fragments arranged by size: 

                 289      96      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     199     417 
   Size:        199     218
 
MspA1I CmG'CkG

Cuts at:      0     160     417 
   Size:        160     257
 
PpuMI rG'GwC_Cy

Cuts at:      0     238     342     417 
   Size:        238     104      75

  Fragments arranged by size: 

                 238     104      75
 
PsiI TTA'TAA

Cuts at:      0     146     417 
   Size:        146     271
 
PvuII CAG'CTG

Cuts at:      0     160     417 
   Size:        160     257
 
SanDI GG'GwC_CC

Cuts at:      0     342     417 
   Size:        342      75
 
SmlI C'TyrA_G

Cuts at:      0     260     417 
   Size:        260     157
 
StyI C'CwwG_G

Cuts at:      0      86     417 
   Size:         86     331
 
TatI w'GTAC_w

Cuts at:      0     408     417 
   Size:        408       9
 
XbaI T'CTAG_A

Cuts at:      0      40     417 
   Size:         40     377
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     335     417 
   Size:        335      82
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI     BanI     BbsI     BglI     BmrI    BpuEI
   BstAPI    BstXI     DraI   DraIII  Eco57MI EcoO109I    EcoRI    Hin4I
     MmeI   MspA1I    PpuMI     PsiI    PvuII    SanDI     SmlI     StyI
     TatI     XbaI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TstI  Tth111I     XhoI     XmaI     XmnI
     ZraI