Restriction map for
pF1KB8742
(Linear) (Six-base) MAPSORT of: KIBB8742.seq Check: 7166 from: 1 to: 1125
KIBB8742.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AfeI AGC'GCT
Cuts at: 0 873 1125
Size: 873 252
AhdI GACnn_n'nnGTC
Cuts at: 0 82 1125
Size: 82 1043
AleI CACnn'nnGTG
Cuts at: 0 220 1125
Size: 220 905
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 664 1125
Size: 664 461
AlwNI CAG_nnn'CTG
Cuts at: 0 545 1036 1125
Size: 545 491 89
Fragments arranged by size:
545 491 89
ApoI r'AATT_y
Cuts at: 0 706 1125
Size: 706 419
AvaI C'yCGr_G
Cuts at: 0 1051 1125
Size: 1051 74
BanII G_rGCy'C
Cuts at: 0 65 299 736 787 1125
Size: 65 234 437 51 338
Fragments arranged by size:
437 338 234 65 51
BglII A'GATC_T
Cuts at: 0 470 1125
Size: 470 655
Bme1580I G_kGCm'C
Cuts at: 0 104 1125
Size: 104 1021
BmgBI CAC'GTC
Cuts at: 0 660 1125
Size: 660 465
BmrI ACTGGGnnnn_n'
Cuts at: 0 353 927 1125
Size: 353 574 198
Fragments arranged by size:
574 353 198
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 49 1125
Size: 49 1076
BsaI GGTCTCn'nnnn_
Cuts at: 0 520 788 1125
Size: 520 268 337
Fragments arranged by size:
520 337 268
BsaAI yAC'GTr
Cuts at: 0 714 1125
Size: 714 411
BsaBI GATnn'nnATC
Cuts at: 0 469 475 1125
Size: 469 6 650
Fragments arranged by size:
650 469 6
BsaWI w'CCGG_w
Cuts at: 0 481 1125
Size: 481 644
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 703 733 811 841 1125
Size: 703 30 78 30 284
Fragments arranged by size:
703 284 78 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 676 711 714 835 1069 1125
Size: 676 35 3 121 234 56
Fragments arranged by size:
676 234 121 56 35 3
BseYI C'CCAG_C
Cuts at: 0 52 58 1125
Size: 52 6 1067
Fragments arranged by size:
1067 52 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 569 1049 1125
Size: 569 480 76
Fragments arranged by size:
569 480 76
BsmBI CGTCTCn'nnnn_
Cuts at: 0 772 1125
Size: 772 353
Bsp1286I G_dGCh'C
Cuts at: 0 65 104 299 736 787 1125
Size: 65 39 195 437 51 338
Fragments arranged by size:
437 338 195 65 51 39
BspHI T'CATG_A
Cuts at: 0 323 1125
Size: 323 802
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 158 1125
Size: 158 967
BsrBI CCG'CTC
Cuts at: 0 678 1125
Size: 678 447
BsrDI GCAATG_nn'
Cuts at: 0 1125
Size: 1125
BstXI CCAn_nnnn'nTGG
Cuts at: 0 637 1125
Size: 637 488
BstYI r'GATC_y
Cuts at: 0 470 1125
Size: 470 655
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 246 1125
Size: 246 879
BtsI GCAGTG_nn'
Cuts at: 0 47 1125
Size: 47 1078
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 142 177 1125
Size: 142 35 948
Fragments arranged by size:
948 142 35
EaeI y'GGCC_r
Cuts at: 0 494 1125
Size: 494 631
EarI CTCTTCn'nnn_
Cuts at: 0 604 1125
Size: 604 521
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 597 633 1125
Size: 597 36 492
Fragments arranged by size:
597 492 36
HaeII r_GCGC'y
Cuts at: 0 875 1125
Size: 875 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 68 100 182 214 709 741 1125
Size: 68 32 82 32 495 32 384
Fragments arranged by size:
495 384 82 68 32 32 32
MscI TGG'CCA
Cuts at: 0 496 1125
Size: 496 629
MslI CAynn'nnrTG
Cuts at: 0 19 220 328 1125
Size: 19 201 108 797
Fragments arranged by size:
797 201 108 19
NspI r_CATG'y
Cuts at: 0 24 1125
Size: 24 1101
PflMI CCAn_nnn'nTGG
Cuts at: 0 771 1125
Size: 771 354
PmlI CAC'GTG
Cuts at: 0 714 1125
Size: 714 411
PshAI GACnn'nnGTC
Cuts at: 0 658 1125
Size: 658 467
PspXI vC'TCGA_Gb
Cuts at: 0 1051 1125
Size: 1051 74
PstI C_TGCA'G
Cuts at: 0 653 1125
Size: 653 472
SapI GCTCTTCn'nnn_
Cuts at: 0 604 1125
Size: 604 521
SexAI A'CCwGG_T
Cuts at: 0 229 1125
Size: 229 896
SfcI C'TryA_G
Cuts at: 0 649 1125
Size: 649 476
SmlI C'TyrA_G
Cuts at: 0 64 1051 1125
Size: 64 987 74
Fragments arranged by size:
987 74 64
StyI C'CwwG_G
Cuts at: 0 665 1125
Size: 665 460
TatI w'GTAC_w
Cuts at: 0 1061 1125
Size: 1061 64
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 703 735 953 985 1125
Size: 703 32 218 32 140
Fragments arranged by size:
703 218 140 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 33 379 432 1125
Size: 33 346 53 693
Fragments arranged by size:
693 346 53 33
XhoI C'TCGA_G
Cuts at: 0 1051 1125
Size: 1051 74
XmnI GAAnn'nnTTC
Cuts at: 0 995 1125
Size: 995 130
Enzymes that do cut:
AfeI AhdI AleI AlfI AlwNI ApoI AvaI BanII
BglII Bme1580I BmgBI BmrI BpuEI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BseYI BsgI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BstXI BstYI BtgZI BtsI CspCI
EaeI EarI FalI HaeII Hin4I MscI MslI NspI
PflMI PmlI PshAI PspXI PstI SapI SexAI SfcI
SmlI StyI TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI BmtI BplI BpmI Bpu10I
BsaHI BsiEI BsiHKAI BsiWI BsmI BspEI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
ClaI DraI DraIII DrdI EagI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PpiI PpuMI
PsiI PspOMI PsrI PvuI PvuII RsrII SacI SacII
SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII Tth111I XbaI XmaI ZraI