(Linear) (Six-base) MAPSORT of: KIBB8743.seq  Check: 8429  from: 1  to: 369



KIBB8743.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AfeI AGC'GCT

Cuts at:      0     238     369 
   Size:        238     131
 
AhdI GACnn_n'nnGTC

Cuts at:      0     170     369 
   Size:        170     199
 
ApaI G_GGCC'C

Cuts at:      0     289     369 
   Size:        289      80
 
AvaI C'yCGr_G

Cuts at:      0      12     232     369 
   Size:         12     220     137

  Fragments arranged by size: 

                 220     137      12
 
BanI G'GyrC_C

Cuts at:      0     341     347     369 
   Size:        341       6      22

  Fragments arranged by size: 

                 341      22       6
 
BanII G_rGCy'C

Cuts at:      0      48     289     369 
   Size:         48     241      80

  Fragments arranged by size: 

                 241      80      48
 
BglI GCCn_nnn'nGGC

Cuts at:      0      16     369 
   Size:         16     353
 
Bme1580I G_kGCm'C

Cuts at:      0     289     352     369 
   Size:        289      63      17

  Fragments arranged by size: 

                 289      63      17
 
BmgBI CAC'GTC

Cuts at:      0     120     369 
   Size:        120     249
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      40     369 
   Size:         40     329
 
BsaHI Gr'CG_yC

Cuts at:      0       4     146     294     369 
   Size:          4     142     148      75

  Fragments arranged by size: 

                 148     142      75       4
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     174     369 
   Size:        174     195
 
BseYI C'CCAG_C

Cuts at:      0      62     245     369 
   Size:         62     183     124

  Fragments arranged by size: 

                 183     124      62
 
BsiEI CG_ry'CG

Cuts at:      0      52     360     369 
   Size:         52     308       9

  Fragments arranged by size: 

                 308      52       9
 
Bsp1286I G_dGCh'C

Cuts at:      0      48     289     352     369 
   Size:         48     241      63      17

  Fragments arranged by size: 

                 241      63      48      17
 
BsrFI r'CCGG_y

Cuts at:      0     344     369 
   Size:        344      25
 
BssHII G'CGCG_C

Cuts at:      0     314     369 
   Size:        314      55
 
BtgI C'CryG_G

Cuts at:      0      20      52     289     369 
   Size:         20      32     237      80

  Fragments arranged by size: 

                 237      80      32      20
 
EaeI y'GGCC_r

Cuts at:      0      49     369 
   Size:         49     320
 
EagI C'GGCC_G

Cuts at:      0      49     369 
   Size:         49     320
 
EcoO109I rG'GnC_Cy

Cuts at:      0     131     285     369 
   Size:        131     154      84

  Fragments arranged by size: 

                 154     131      84
 
HaeII r_GCGC'y

Cuts at:      0     222     240     369 
   Size:        222      18     129

  Fragments arranged by size: 

                 222     129      18
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     156     188     369 
   Size:        156      32     181

  Fragments arranged by size: 

                 181     156      32
 
MspA1I CmG'CkG

Cuts at:      0      54      62     291     369 
   Size:         54       8     229      78

  Fragments arranged by size: 

                 229      78      54       8
 
NspI r_CATG'y

Cuts at:      0     326     369 
   Size:        326      43
 
PpuMI rG'GwC_Cy

Cuts at:      0     131     369 
   Size:        131     238
 
PspOMI G'GGCC_C

Cuts at:      0     285     369 
   Size:        285      84
 
SacII CC_GC'GG

Cuts at:      0      55     292     369 
   Size:         55     237      77

  Fragments arranged by size: 

                 237      77      55
 
SanDI GG'GwC_CC

Cuts at:      0     131     369 
   Size:        131     238
 
SfcI C'TryA_G

Cuts at:      0     309     369 
   Size:        309      60
 
SphI G_CATG'C

Cuts at:      0     326     369 
   Size:        326      43
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      27     369 
   Size:         27     342
 

 Enzymes that do cut:

     AfeI     AhdI     ApaI     AvaI     BanI    BanII     BglI Bme1580I
    BmgBI     BmrI    BsaHI    BseRI    BseYI    BsiEI Bsp1286I    BsrFI
   BssHII     BtgI     EaeI     EagI EcoO109I    HaeII    Hin4I   MspA1I
     NspI    PpuMI   PspOMI    SacII    SanDI     SfcI     SphI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmtI     BplI
     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI
     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI    BssSI   BstAPI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EarI     EciI  EcoICRI  Eco57MI    EcoNI    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     SspI
     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI     ZraI