(Linear) (Six-base) MAPSORT of: KIBB8744.seq  Check: 4048  from: 1  to: 381



KIBB8744.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     198     381 
   Size:        198     183
 
AhdI GACnn_n'nnGTC

Cuts at:      0     117     381 
   Size:        117     264
 
AleI CACnn'nnGTG

Cuts at:      0     254     381 
   Size:        254     127
 
AlwNI CAG_nnn'CTG

Cuts at:      0      81     381 
   Size:         81     300
 
AvaI C'yCGr_G

Cuts at:      0      58     252     381 
   Size:         58     194     129

  Fragments arranged by size: 

                 194     129      58
 
BanII G_rGCy'C

Cuts at:      0      59     350     381 
   Size:         59     291      31

  Fragments arranged by size: 

                 291      59      31
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     368     381 
   Size:        368      13
 
BbvCI CC'TCA_GC

Cuts at:      0     299     381 
   Size:        299      82
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     381 
   Size:         73     308
 
Bpu10I CC'TnA_GC

Cuts at:      0     299     381 
   Size:        299      82
 
BsaAI yAC'GTr

Cuts at:      0     243     381 
   Size:        243     138
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      76     238     316     381 
   Size:         76     162      78      65

  Fragments arranged by size: 

                 162      78      76      65
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     265     381 
   Size:        265     116
 
BsiHKAI G_wGCw'C

Cuts at:      0     137     350     381 
   Size:        137     213      31

  Fragments arranged by size: 

                 213     137      31
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     125     381 
   Size:        125     256
 
Bsp1286I G_dGCh'C

Cuts at:      0      59     137     350     381 
   Size:         59      78     213      31

  Fragments arranged by size: 

                 213      78      59      31
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     158     198     381 
   Size:        158      40     183

  Fragments arranged by size: 

                 183     158      40
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      81     381 
   Size:         81     300
 
Bsu36I CC'TnA_GG

Cuts at:      0      35     381 
   Size:         35     346
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      99     381 
   Size:         99     282
 
EcoICRI GAG'CTC

Cuts at:      0     348     381 
   Size:        348      33
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      73     381 
   Size:         73     308
 
HaeII r_GCGC'y

Cuts at:      0      34     233     381 
   Size:         34     199     148

  Fragments arranged by size: 

                 199     148      34
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     103     135     262     294     381 
   Size:        103      32     127      32      87

  Fragments arranged by size: 

                 127     103      87      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     260     381 
   Size:        260     121
 
MslI CAynn'nnrTG

Cuts at:      0     254     381 
   Size:        254     127
 
PmlI CAC'GTG

Cuts at:      0     243     381 
   Size:        243     138
 
SacI G_AGCT'C

Cuts at:      0     350     381 
   Size:        350      31
 
StuI AGG'CCT

Cuts at:      0     217     381 
   Size:        217     164
 
StyI C'CwwG_G

Cuts at:      0     311     381 
   Size:        311      70
 

 Enzymes that do cut:

     AarI     AhdI     AleI    AlwNI     AvaI    BanII     BbsI    BbvCI
     BpmI   Bpu10I    BsaAI    BseRI     BsgI  BsiHKAI    BsmBI Bsp1286I
    BspMI   BstAPI   Bsu36I    BtgZI  EcoICRI  Eco57MI    HaeII    Hin4I
     MmeI     MslI     PmlI     SacI     StuI     StyI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI     AseI
    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BplI    BpuEI     BsaI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI
  BstZ17I     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI  Tth111I
     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI