Restriction map for
pF1KB8749
(Linear) (Six-base) MAPSORT of: KIBB8749.seq Check: 1612 from: 1 to: 1254
KIBB8749.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AccI GT'mk_AC
Cuts at: 0 134 1254
Size: 134 1120
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 1254
Size: 440 814
AflII C'TTAA_G
Cuts at: 0 465 1254
Size: 465 789
AleI CACnn'nnGTG
Cuts at: 0 193 1254
Size: 193 1061
AlwNI CAG_nnn'CTG
Cuts at: 0 1145 1254
Size: 1145 109
ApoI r'AATT_y
Cuts at: 0 908 1254
Size: 908 346
AvaI C'yCGr_G
Cuts at: 0 902 1254
Size: 902 352
BanI G'GyrC_C
Cuts at: 0 209 1254
Size: 209 1045
BanII G_rGCy'C
Cuts at: 0 537 654 784 1254
Size: 537 117 130 470
Fragments arranged by size:
537 470 130 117
BbeI G_GCGC'C
Cuts at: 0 213 1254
Size: 213 1041
BbsI GAAGACnn'nnnn_
Cuts at: 0 105 680 1254
Size: 105 575 574
Fragments arranged by size:
575 574 105
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1067 1101 1254
Size: 1067 34 153
Fragments arranged by size:
1067 153 34
BglI GCCn_nnn'nGGC
Cuts at: 0 514 1254
Size: 514 740
BmgBI CAC'GTC
Cuts at: 0 1091 1254
Size: 1091 163
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 928 1254
Size: 928 326
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 1254
Size: 439 815
BsaI GGTCTCn'nnnn_
Cuts at: 0 373 1156 1254
Size: 373 783 98
Fragments arranged by size:
783 373 98
BsaAI yAC'GTr
Cuts at: 0 630 1254
Size: 630 624
BsaHI Gr'CG_yC
Cuts at: 0 210 1254
Size: 210 1044
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1174 1254
Size: 1174 80
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1071 1145 1254
Size: 1071 74 109
Fragments arranged by size:
1071 109 74
BsiHKAI G_wGCw'C
Cuts at: 0 71 443 1254
Size: 71 372 811
Fragments arranged by size:
811 372 71
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1101 1254
Size: 1101 153
Bsp1286I G_dGCh'C
Cuts at: 0 71 443 537 654 784 1254
Size: 71 372 94 117 130 470
Fragments arranged by size:
470 372 130 117 94 71
BssSI C'ACGA_G
Cuts at: 0 249 1254
Size: 249 1005
BstEII G'GTnAC_C
Cuts at: 0 741 1254
Size: 741 513
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 1029 1254
Size: 195 834 225
Fragments arranged by size:
834 225 195
Bsu36I CC'TnA_GG
Cuts at: 0 1157 1254
Size: 1157 97
BtsI GCAGTG_nn'
Cuts at: 0 1023 1254
Size: 1023 231
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 611 646 1254
Size: 611 35 608
Fragments arranged by size:
611 608 35
DraI TTT'AAA
Cuts at: 0 705 1226 1254
Size: 705 521 28
Fragments arranged by size:
705 521 28
DraIII CAC_nnn'GTG
Cuts at: 0 206 331 1254
Size: 206 125 923
Fragments arranged by size:
923 206 125
EaeI y'GGCC_r
Cuts at: 0 434 506 619 1254
Size: 434 72 113 635
Fragments arranged by size:
635 434 113 72
EarI CTCTTCn'nnn_
Cuts at: 0 79 84 1254
Size: 79 5 1170
Fragments arranged by size:
1170 79 5
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1164 1254
Size: 1164 90
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 439 440 1254
Size: 439 1 814
Fragments arranged by size:
814 439 1
EcoO109I rG'GnC_Cy
Cuts at: 0 359 1254
Size: 359 895
HaeII r_GCGC'y
Cuts at: 0 213 1254
Size: 213 1041
HincII GTy'rAC
Cuts at: 0 177 336 723 1254
Size: 177 159 387 531
Fragments arranged by size:
531 387 177 159
HindIII A'AGCT_T
Cuts at: 0 586 1254
Size: 586 668
KasI G'GCGC_C
Cuts at: 0 209 1254
Size: 209 1045
MfeI C'AATT_G
Cuts at: 0 1027 1254
Size: 1027 227
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1073 1254
Size: 1073 181
MscI TGG'CCA
Cuts at: 0 508 1254
Size: 508 746
MslI CAynn'nnrTG
Cuts at: 0 116 193 1254
Size: 116 77 1061
Fragments arranged by size:
1061 116 77
MspA1I CmG'CkG
Cuts at: 0 125 1145 1254
Size: 125 1020 109
Fragments arranged by size:
1020 125 109
NarI GG'CG_CC
Cuts at: 0 210 1254
Size: 210 1044
NdeI CA'TA_TG
Cuts at: 0 215 1254
Size: 215 1039
PmlI CAC'GTG
Cuts at: 0 630 1254
Size: 630 624
PshAI GACnn'nnGTC
Cuts at: 0 1113 1254
Size: 1113 141
PvuII CAG'CTG
Cuts at: 0 125 1145 1254
Size: 125 1020 109
Fragments arranged by size:
1020 125 109
SapI GCTCTTCn'nnn_
Cuts at: 0 84 1254
Size: 84 1170
SfcI C'TryA_G
Cuts at: 0 1238 1254
Size: 1238 16
SfoI GGC'GCC
Cuts at: 0 211 1254
Size: 211 1043
SmlI C'TyrA_G
Cuts at: 0 465 1254
Size: 465 789
StyI C'CwwG_G
Cuts at: 0 503 1100 1254
Size: 503 597 154
Fragments arranged by size:
597 503 154
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 155 187 190 222 1254
Size: 155 32 3 32 1032
Fragments arranged by size:
1032 155 32 32 3
Tth111I GACn'n_nGTC
Cuts at: 0 340 1254
Size: 340 914
XbaI T'CTAG_A
Cuts at: 0 883 1254
Size: 883 371
Enzymes that do cut:
AccI AcuI AflII AleI AlwNI ApoI AvaI BanI
BanII BbeI BbsI BcgI BglI BmgBI BplI BpmI
BsaI BsaAI BsaHI BseRI BsgI BsiHKAI BsmBI Bsp1286I
BssSI BstEII BstXI Bsu36I BtsI CspCI DraI DraIII
EaeI EarI EciI Eco57MI EcoO109I HaeII HincII HindIII
KasI MfeI MmeI MscI MslI MspA1I NarI NdeI
PmlI PshAI PvuII SapI SfcI SfoI SmlI StyI
TstI Tth111I XbaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflIII AgeI AhdI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI
Bme1580I BmrI BmtI Bpu10I BpuEI BsaBI BsaWI BsaXI
BseYI BsiEI BsiWI BsmI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstYI BstZ17I
BtgI BtgZI ClaI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HpaI KpnI
MluI NaeI NcoI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PpiI
PpuMI PsiI PspOMI PspXI PsrI PstI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI XcmI XhoI XmaI XmnI
ZraI