(Linear) (Six-base) MAPSORT of: KIBB8750.seq  Check: 2499  from: 1  to: 420



KIBB8750.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0      68     224     420 
   Size:         68     156     196

  Fragments arranged by size: 

                 196     156      68
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     408     420 
   Size:        408      12
 
BbvCI CC'TCA_GC

Cuts at:      0      50     420 
   Size:         50     370
 
Bme1580I G_kGCm'C

Cuts at:      0     124     420 
   Size:        124     296
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     420 
   Size:        420
 
Bpu10I CC'TnA_GC

Cuts at:      0      50     420 
   Size:         50     370
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     192     420 
   Size:        192     228
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      67     420 
   Size:         67     353
 
BseYI C'CCAG_C

Cuts at:      0     361     420 
   Size:        361      59
 
Bsp1286I G_dGCh'C

Cuts at:      0     124     420 
   Size:        124     296
 
BspHI T'CATG_A

Cuts at:      0     240     420 
   Size:        240     180
 
BsrDI GCAATG_nn'

Cuts at:      0     153     420 
   Size:        153     267
 
BsrGI T'GTAC_A

Cuts at:      0     113     420 
   Size:        113     307
 
BtgI C'CryG_G

Cuts at:      0      80     266     420 
   Size:         80     186     154

  Fragments arranged by size: 

                 186     154      80
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     255     290     420 
   Size:        255      35     130

  Fragments arranged by size: 

                 255     130      35
 
DraIII CAC_nnn'GTG

Cuts at:      0     374     420 
   Size:        374      46
 
MslI CAynn'nnrTG

Cuts at:      0      79     420 
   Size:         79     341
 
NcoI C'CATG_G

Cuts at:      0      80     420 
   Size:         80     340
 
PfoI T'CCnGG_A

Cuts at:      0     404     420 
   Size:        404      16
 
PshAI GACnn'nnGTC

Cuts at:      0     268     420 
   Size:        268     152
 
PstI C_TGCA'G

Cuts at:      0      43     420 
   Size:         43     377
 
SfcI C'TryA_G

Cuts at:      0      39     420 
   Size:         39     381
 
SmlI C'TyrA_G

Cuts at:      0     171     420 
   Size:        171     249
 
StuI AGG'CCT

Cuts at:      0      37     355     420 
   Size:         37     318      65

  Fragments arranged by size: 

                 318      65      37
 
StyI C'CwwG_G

Cuts at:      0      80     420 
   Size:         80     340
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     375     420 
   Size:        375      45
 
TatI w'GTAC_w

Cuts at:      0     113     420 
   Size:        113     307
 

 Enzymes that do cut:

     AccI     AlfI    BbvCI Bme1580I     BmrI   Bpu10I    BpuEI    BseRI
    BseYI Bsp1286I    BspHI    BsrDI    BsrGI     BtgI    CspCI   DraIII
     MslI     NcoI     PfoI    PshAI     PstI     SfcI     SmlI     StuI
     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmtI     BplI     BpmI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI