(Linear) (Six-base) MAPSORT of: KIBB8751.seq  Check: 1510  from: 1  to: 552



KIBB8751.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     372     552 
   Size:        372     180
 
ApoI r'AATT_y

Cuts at:      0     142     153     229     403     552 
   Size:        142      11      76     174     149

  Fragments arranged by size: 

                 174     149     142      76      11
 
AvaI C'yCGr_G

Cuts at:      0     197     552 
   Size:        197     355
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     136     552 
   Size:        136     416
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     552 
   Size:         31     521
 
BsaWI w'CCGG_w

Cuts at:      0     480     552 
   Size:        480      72
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     498     552 
   Size:        498      54
 
BseYI C'CCAG_C

Cuts at:      0     306     552 
   Size:        306     246
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     338     552 
   Size:        338     214
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     135     552 
   Size:        135     417
 
BtsI GCAGTG_nn'

Cuts at:      0      85     552 
   Size:         85     467
 
DraI TTT'AAA

Cuts at:      0     225     552 
   Size:        225     327
 
EaeI y'GGCC_r

Cuts at:      0      69     147     460     552 
   Size:         69      78     313      92

  Fragments arranged by size: 

                 313      92      78      69
 
EarI CTCTTCn'nnn_

Cuts at:      0      23     552 
   Size:         23     529
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      31     552 
   Size:         31     521
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     379     411     552 
   Size:        379      32     141

  Fragments arranged by size: 

                 379     141      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     550     552 
   Size:        182     368       2

  Fragments arranged by size: 

                 368     182       2
 
MscI TGG'CCA

Cuts at:      0      71     462     552 
   Size:         71     391      90

  Fragments arranged by size: 

                 391      90      71
 
MslI CAynn'nnrTG

Cuts at:      0     339     552 
   Size:        339     213
 
MspA1I CmG'CkG

Cuts at:      0     219     552 
   Size:        219     333
 
PstI C_TGCA'G

Cuts at:      0     218     552 
   Size:        218     334
 
PvuII CAG'CTG

Cuts at:      0     219     552 
   Size:        219     333
 
SfcI C'TryA_G

Cuts at:      0     214     552 
   Size:        214     338
 
SspI AAT'ATT

Cuts at:      0      89     552 
   Size:         89     463
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      79     288     552 
   Size:         79     209     264

  Fragments arranged by size: 

                 264     209      79
 

 Enzymes that do cut:

    AlwNI     ApoI     AvaI     BplI     BpmI    BsaWI    BseRI    BseYI
    BspMI    BstXI     BtsI     DraI     EaeI     EarI  Eco57MI    Hin4I
     MmeI     MscI     MslI   MspA1I     PstI    PvuII     SfcI     SspI
     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaXI     BsgI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI   DraIII     DrdI
     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     XmnI     ZraI