(Linear) (Six-base) MAPSORT of: KIBB8752.seq  Check: 238  from: 1  to: 570



KIBB8752.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     539     570 
   Size:        539      31
 
AlwNI CAG_nnn'CTG

Cuts at:      0     193     570 
   Size:        193     377
 
ApaLI G'TGCA_C

Cuts at:      0     146     570 
   Size:        146     424
 
BanII G_rGCy'C

Cuts at:      0     464     570 
   Size:        464     106
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     315     570 
   Size:        315     255
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     297     570 
   Size:        297     273
 
Bme1580I G_kGCm'C

Cuts at:      0     150     570 
   Size:        150     420
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     458     570 
   Size:        458     112
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     570 
   Size:        308     262
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     232     570 
   Size:        232     338
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     426     456     570 
   Size:        426      30     114

  Fragments arranged by size: 

                 426     114      30
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     498     570 
   Size:        498      72
 
BsiHKAI G_wGCw'C

Cuts at:      0     150     570 
   Size:        150     420
 
Bsp1286I G_dGCh'C

Cuts at:      0     150     464     570 
   Size:        150     314     106

  Fragments arranged by size: 

                 314     150     106
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     193     476     570 
   Size:        193     283      94

  Fragments arranged by size: 

                 283     193      94
 
BstZ17I GTA'TAC

Cuts at:      0     540     570 
   Size:        540      30
 
BtgI C'CryG_G

Cuts at:      0      91     570 
   Size:         91     479
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     264     570 
   Size:        264     306
 
DraIII CAC_nnn'GTG

Cuts at:      0     476     570 
   Size:        476      94
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      24     570 
   Size:         24     546
 
EaeI y'GGCC_r

Cuts at:      0      94     570 
   Size:         94     476
 
EarI CTCTTCn'nnn_

Cuts at:      0      81     321     570 
   Size:         81     240     249

  Fragments arranged by size: 

                 249     240      81
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     308     570 
   Size:        308     262
 
EcoO109I rG'GnC_Cy

Cuts at:      0     524     570 
   Size:        524      46
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     332     570 
   Size:        332     238
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     569     570 
   Size:        569       1
 
MscI TGG'CCA

Cuts at:      0      96     570 
   Size:         96     474
 
NcoI C'CATG_G

Cuts at:      0      91     570 
   Size:         91     479
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     425     570 
   Size:        425     145
 
PpuMI rG'GwC_Cy

Cuts at:      0     524     570 
   Size:        524      46
 
SfcI C'TryA_G

Cuts at:      0     533     570 
   Size:        533      37
 
StuI AGG'CCT

Cuts at:      0     107     570 
   Size:        107     463
 
StyI C'CwwG_G

Cuts at:      0      91     108     570 
   Size:         91      17     462

  Fragments arranged by size: 

                 462      91      17
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     114     136     570 
   Size:        114      22     434

  Fragments arranged by size: 

                 434     114      22
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     111     143     426     458     570 
   Size:        111      32     283      32     112

  Fragments arranged by size: 

                 283     112     111      32      32
 

 Enzymes that do cut:

     AccI    AlwNI    ApaLI    BanII     BbsI    BciVI Bme1580I     BmrI
     BpmI     BsaI    BsaXI     BsgI  BsiHKAI Bsp1286I   BstAPI  BstZ17I
     BtgI    BtgZI   DraIII     DrdI     EaeI     EarI  Eco57MI EcoO109I
     FalI     MmeI     MscI     NcoI    PflMI    PpuMI     SfcI     StuI
     StyI    TaqII     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI     ApoI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
    BbvCI     BcgI     BclI    BfrBI     BglI    BglII     BlpI    BmgBI
     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI
   BstEII    BstXI    BstYI   Bsu36I     BtsI     ClaI    CspCI     DraI
     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI