Restriction map for
pF1KB8753
(Linear) (Six-base) MAPSORT of: KIBB8753.seq Check: 1278 from: 1 to: 588
KIBB8753.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 588
Size: 115 473
AflII C'TTAA_G
Cuts at: 0 397 588
Size: 397 191
AflIII A'CryG_T
Cuts at: 0 167 588
Size: 167 421
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 588
Size: 556 32
AlwNI CAG_nnn'CTG
Cuts at: 0 504 588
Size: 504 84
ApoI r'AATT_y
Cuts at: 0 12 588
Size: 12 576
AvaI C'yCGr_G
Cuts at: 0 289 487 588
Size: 289 198 101
Fragments arranged by size:
289 198 101
BanI G'GyrC_C
Cuts at: 0 375 588
Size: 375 213
BanII G_rGCy'C
Cuts at: 0 308 452 555 588
Size: 308 144 103 33
Fragments arranged by size:
308 144 103 33
BglI GCCn_nnn'nGGC
Cuts at: 0 559 588
Size: 559 29
BglII A'GATC_T
Cuts at: 0 583 588
Size: 583 5
BlpI GC'TnA_GC
Cuts at: 0 302 563 588
Size: 302 261 25
Fragments arranged by size:
302 261 25
Bme1580I G_kGCm'C
Cuts at: 0 167 468 588
Size: 167 301 120
Fragments arranged by size:
301 167 120
BmrI ACTGGGnnnn_n'
Cuts at: 0 482 588
Size: 482 106
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 588
Size: 109 479
BsaAI yAC'GTr
Cuts at: 0 168 258 588
Size: 168 90 330
Fragments arranged by size:
330 168 90
BsaHI Gr'CG_yC
Cuts at: 0 341 588
Size: 341 247
BseYI C'CCAG_C
Cuts at: 0 443 588
Size: 443 145
BsiHKAI G_wGCw'C
Cuts at: 0 308 452 588
Size: 308 144 136
Fragments arranged by size:
308 144 136
Bsp1286I G_dGCh'C
Cuts at: 0 167 308 452 468 555 588
Size: 167 141 144 16 87 33
Fragments arranged by size:
167 144 141 87 33 16
BsrBI CCG'CTC
Cuts at: 0 207 588
Size: 207 381
BssSI C'ACGA_G
Cuts at: 0 250 588
Size: 250 338
BstAPI GCAn_nnn'nTGC
Cuts at: 0 382 588
Size: 382 206
BstYI r'GATC_y
Cuts at: 0 583 588
Size: 583 5
BtgI C'CryG_G
Cuts at: 0 555 588
Size: 555 33
BtsI GCAGTG_nn'
Cuts at: 0 468 588
Size: 468 120
EaeI y'GGCC_r
Cuts at: 0 359 588
Size: 359 229
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 46 588
Size: 46 542
EcoICRI GAG'CTC
Cuts at: 0 306 450 588
Size: 306 144 138
Fragments arranged by size:
306 144 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 588
Size: 115 473
EcoO109I rG'GnC_Cy
Cuts at: 0 201 492 588
Size: 201 291 96
Fragments arranged by size:
291 201 96
EcoRI G'AATT_C
Cuts at: 0 12 588
Size: 12 576
EcoRV GAT'ATC
Cuts at: 0 246 588
Size: 246 342
MscI TGG'CCA
Cuts at: 0 361 588
Size: 361 227
MspA1I CmG'CkG
Cuts at: 0 108 218 393 443 530 588
Size: 108 110 175 50 87 58
Fragments arranged by size:
175 110 108 87 58 50
NcoI C'CATG_G
Cuts at: 0 555 588
Size: 555 33
NspI r_CATG'y
Cuts at: 0 537 588
Size: 537 51
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 111 588
Size: 108 3 477
Fragments arranged by size:
477 108 3
PfoI T'CCnGG_A
Cuts at: 0 431 588
Size: 431 157
PmlI CAC'GTG
Cuts at: 0 168 588
Size: 168 420
PstI C_TGCA'G
Cuts at: 0 463 500 545 588
Size: 463 37 45 43
Fragments arranged by size:
463 45 43 37
PvuII CAG'CTG
Cuts at: 0 108 218 530 588
Size: 108 110 312 58
Fragments arranged by size:
312 110 108 58
SacI G_AGCT'C
Cuts at: 0 308 452 588
Size: 308 144 136
Fragments arranged by size:
308 144 136
SfcI C'TryA_G
Cuts at: 0 459 496 541 588
Size: 459 37 45 47
Fragments arranged by size:
459 47 45 37
SmlI C'TyrA_G
Cuts at: 0 397 588
Size: 397 191
StyI C'CwwG_G
Cuts at: 0 555 588
Size: 555 33
XbaI T'CTAG_A
Cuts at: 0 420 588
Size: 420 168
Enzymes that do cut:
AcuI AflII AflIII AlfI AlwNI ApoI AvaI BanI
BanII BglI BglII BlpI Bme1580I BmrI BsaI BsaAI
BsaHI BseYI BsiHKAI Bsp1286I BsrBI BssSI BstAPI BstYI
BtgI BtsI EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV MscI MspA1I NcoI NspI PflMI PfoI PmlI
PstI PvuII SacI SfcI SmlI StyI XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AloI ApaI ApaLI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaBI
BsaWI BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EarI EcoNI FalI FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MslI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XcmI XhoI XmaI XmnI ZraI