Restriction map for
pF1KB8754
(Linear) (Six-base) MAPSORT of: KIBB8754.seq Check: 5606 from: 1 to: 594
KIBB8754.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 126 594
Size: 126 468
AfeI AGC'GCT
Cuts at: 0 541 594
Size: 541 53
AlwNI CAG_nnn'CTG
Cuts at: 0 219 279 489 594
Size: 219 60 210 105
Fragments arranged by size:
219 210 105 60
ApaI G_GGCC'C
Cuts at: 0 296 594
Size: 296 298
ApaLI G'TGCA_C
Cuts at: 0 162 594
Size: 162 432
BanII G_rGCy'C
Cuts at: 0 64 296 479 594
Size: 64 232 183 115
Fragments arranged by size:
232 183 115 64
BclI T'GATC_A
Cuts at: 0 140 594
Size: 140 454
Bme1580I G_kGCm'C
Cuts at: 0 91 166 296 594
Size: 91 75 130 298
Fragments arranged by size:
298 130 91 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 520 594
Size: 301 219 74
Fragments arranged by size:
301 219 74
BsaI GGTCTCn'nnnn_
Cuts at: 0 436 594
Size: 436 158
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 412 442 594
Size: 412 30 152
Fragments arranged by size:
412 152 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 594
Size: 298 296
BseYI C'CCAG_C
Cuts at: 0 288 594
Size: 288 306
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 334 594
Size: 334 260
BsiHKAI G_wGCw'C
Cuts at: 0 64 166 479 594
Size: 64 102 313 115
Fragments arranged by size:
313 115 102 64
BsmBI CGTCTCn'nnnn_
Cuts at: 0 367 496 594
Size: 367 129 98
Fragments arranged by size:
367 129 98
Bsp1286I G_dGCh'C
Cuts at: 0 64 91 166 296 479 594
Size: 64 27 75 130 183 115
Fragments arranged by size:
183 130 115 75 64 27
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 126 594
Size: 126 468
BsrFI r'CCGG_y
Cuts at: 0 131 187 594
Size: 131 56 407
Fragments arranged by size:
407 131 56
BssSI C'ACGA_G
Cuts at: 0 352 594
Size: 352 242
BstAPI GCAn_nnn'nTGC
Cuts at: 0 365 594
Size: 365 229
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 383 594
Size: 383 211
EaeI y'GGCC_r
Cuts at: 0 206 449 594
Size: 206 243 145
Fragments arranged by size:
243 206 145
EarI CTCTTCn'nnn_
Cuts at: 0 484 550 594
Size: 484 66 44
Fragments arranged by size:
484 66 44
EcoICRI GAG'CTC
Cuts at: 0 62 477 594
Size: 62 415 117
Fragments arranged by size:
415 117 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 301 520 594
Size: 301 219 74
Fragments arranged by size:
301 219 74
EcoO109I rG'GnC_Cy
Cuts at: 0 292 594
Size: 292 302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 495 594
Size: 495 99
HaeII r_GCGC'y
Cuts at: 0 219 543 594
Size: 219 324 51
Fragments arranged by size:
324 219 51
MslI CAynn'nnrTG
Cuts at: 0 250 343 589 594
Size: 250 93 246 5
Fragments arranged by size:
250 246 93 5
MspA1I CmG'CkG
Cuts at: 0 146 381 594
Size: 146 235 213
Fragments arranged by size:
235 213 146
PfoI T'CCnGG_A
Cuts at: 0 482 544 594
Size: 482 62 50
Fragments arranged by size:
482 62 50
PspOMI G'GGCC_C
Cuts at: 0 292 594
Size: 292 302
PstI C_TGCA'G
Cuts at: 0 173 594
Size: 173 421
PvuII CAG'CTG
Cuts at: 0 146 594
Size: 146 448
SacI G_AGCT'C
Cuts at: 0 64 479 594
Size: 64 415 115
Fragments arranged by size:
415 115 64
SapI GCTCTTCn'nnn_
Cuts at: 0 484 594
Size: 484 110
SfcI C'TryA_G
Cuts at: 0 169 594
Size: 169 425
StyI C'CwwG_G
Cuts at: 0 230 594
Size: 230 364
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 412 444 594
Size: 412 32 150
Fragments arranged by size:
412 150 32
Tth111I GACn'n_nGTC
Cuts at: 0 568 594
Size: 568 26
Enzymes that do cut:
AarI AfeI AlwNI ApaI ApaLI BanII BclI Bme1580I
BpmI BsaI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI
Bsp1286I BspMI BsrFI BssSI BstAPI BtgZI EaeI EarI
EcoICRI Eco57MI EcoO109I FalI HaeII MslI MspA1I PfoI
PspOMI PstI PvuII SacI SapI SfcI StyI TstI
Tth111I
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BfrBI BglI BglII BlpI BmgBI BmrI
BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI
BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI BsrGI
BssHII BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI
BtsI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoNI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI XbaI XcmI XhoI
XmaI XmnI ZraI