(Linear) (Six-base) MAPSORT of: KIBB8756.seq  Check: 8750  from: 1  to: 600



KIBB8756.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

ApoI r'AATT_y

Cuts at:      0     130     192     600 
   Size:        130      62     408

  Fragments arranged by size: 

                 408     130      62
 
BanII G_rGCy'C

Cuts at:      0     472     600 
   Size:        472     128
 
BclI T'GATC_A

Cuts at:      0     554     600 
   Size:        554      46
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     556     600 
   Size:        556      44
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     368     398     600 
   Size:        368      30     202

  Fragments arranged by size: 

                 368     202      30
 
BsiHKAI G_wGCw'C

Cuts at:      0     472     600 
   Size:        472     128
 
Bsp1286I G_dGCh'C

Cuts at:      0     472     600 
   Size:        472     128
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      50     600 
   Size:         50     550
 
BsrDI GCAATG_nn'

Cuts at:      0     461     600 
   Size:        461     139
 
BstEII G'GTnAC_C

Cuts at:      0     180     600 
   Size:        180     420
 
EcoICRI GAG'CTC

Cuts at:      0     470     600 
   Size:        470     130
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     152     600 
   Size:        152     448
 
EcoRV GAT'ATC

Cuts at:      0     362     600 
   Size:        362     238
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     201     600 
   Size:        201     399
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      62     600 
   Size:         62     538
 
PshAI GACnn'nnGTC

Cuts at:      0     103     600 
   Size:        103     497
 
PstI C_TGCA'G

Cuts at:      0     122     600 
   Size:        122     478
 
SacI G_AGCT'C

Cuts at:      0     472     600 
   Size:        472     128
 
SfcI C'TryA_G

Cuts at:      0     118     600 
   Size:        118     482
 
SmlI C'TyrA_G

Cuts at:      0     535     600 
   Size:        535      65
 
StyI C'CwwG_G

Cuts at:      0     185     600 
   Size:        185     415
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     448     600 
   Size:        448     152
 
TatI w'GTAC_w

Cuts at:      0     384     600 
   Size:        384     216
 

 Enzymes that do cut:

     ApoI    BanII     BclI    BpuEI    BsaXI  BsiHKAI Bsp1286I    BspMI
    BsrDI   BstEII  EcoICRI    EcoNI    EcoRV     FalI     MmeI    PshAI
     PstI     SacI     SfcI     SmlI     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI
  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  Eco57MI EcoO109I
    EcoRI     FseI     FspI    FspAI    HaeII    Hin4I   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI     PsiI   PspOMI    PspXI     PsrI     PvuI
    PvuII    RsrII    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI