Restriction map for
pF1KB8757
(Linear) (Six-base) MAPSORT of: KIBB8757.seq Check: 5597 from: 1 to: 603
KIBB8757.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AatII G_ACGT'C
Cuts at: 0 230 603
Size: 230 373
AfeI AGC'GCT
Cuts at: 0 432 507 603
Size: 432 75 96
Fragments arranged by size:
432 96 75
AhdI GACnn_n'nnGTC
Cuts at: 0 284 603
Size: 284 319
AleI CACnn'nnGTG
Cuts at: 0 490 603
Size: 490 113
AvaI C'yCGr_G
Cuts at: 0 190 418 581 603
Size: 190 228 163 22
Fragments arranged by size:
228 190 163 22
BanI G'GyrC_C
Cuts at: 0 24 183 603
Size: 24 159 420
Fragments arranged by size:
420 159 24
BbeI G_GCGC'C
Cuts at: 0 28 603
Size: 28 575
BbvCI CC'TCA_GC
Cuts at: 0 437 603
Size: 437 166
BlpI GC'TnA_GC
Cuts at: 0 317 603
Size: 317 286
Bme1580I G_kGCm'C
Cuts at: 0 188 426 603
Size: 188 238 177
Fragments arranged by size:
238 188 177
BmtI G_CTAG'C
Cuts at: 0 472 603
Size: 472 131
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 603
Size: 346 257
Bpu10I CC'TnA_GC
Cuts at: 0 437 603
Size: 437 166
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 106 547 559 603
Size: 106 441 12 44
Fragments arranged by size:
441 106 44 12
BsaHI Gr'CG_yC
Cuts at: 0 25 227 603
Size: 25 202 376
Fragments arranged by size:
376 202 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 27 603
Size: 27 576
BsmBI CGTCTCn'nnnn_
Cuts at: 0 591 603
Size: 591 12
Bsp1286I G_dGCh'C
Cuts at: 0 188 426 603
Size: 188 238 177
Fragments arranged by size:
238 188 177
BsrBI CCG'CTC
Cuts at: 0 321 603
Size: 321 282
BstYI r'GATC_y
Cuts at: 0 176 603
Size: 176 427
BtsI GCAGTG_nn'
Cuts at: 0 29 603
Size: 29 574
EaeI y'GGCC_r
Cuts at: 0 398 603
Size: 398 205
EarI CTCTTCn'nnn_
Cuts at: 0 442 589 603
Size: 442 147 14
Fragments arranged by size:
442 147 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 603
Size: 346 257
EcoNI CCTnn'n_nnAGG
Cuts at: 0 529 603
Size: 529 74
EcoO109I rG'GnC_Cy
Cuts at: 0 534 603
Size: 534 69
HaeII r_GCGC'y
Cuts at: 0 28 264 434 509 546 603
Size: 28 236 170 75 37 57
Fragments arranged by size:
236 170 75 57 37 28
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 270 302 603
Size: 270 32 301
Fragments arranged by size:
301 270 32
KasI G'GCGC_C
Cuts at: 0 24 603
Size: 24 579
MscI TGG'CCA
Cuts at: 0 400 603
Size: 400 203
MslI CAynn'nnrTG
Cuts at: 0 490 603
Size: 490 113
NarI GG'CG_CC
Cuts at: 0 25 603
Size: 25 578
NheI G'CTAG_C
Cuts at: 0 468 603
Size: 468 135
NspI r_CATG'y
Cuts at: 0 70 603
Size: 70 533
PstI C_TGCA'G
Cuts at: 0 505 530 603
Size: 505 25 73
Fragments arranged by size:
505 73 25
SfcI C'TryA_G
Cuts at: 0 501 526 603
Size: 501 25 77
Fragments arranged by size:
501 77 25
SfoI GGC'GCC
Cuts at: 0 26 603
Size: 26 577
SmaI CCC'GGG
Cuts at: 0 420 603
Size: 420 183
SmlI C'TyrA_G
Cuts at: 0 121 538 562 603
Size: 121 417 24 41
Fragments arranged by size:
417 121 41 24
StyI C'CwwG_G
Cuts at: 0 335 603
Size: 335 268
XmaI C'CCGG_G
Cuts at: 0 418 603
Size: 418 185
ZraI GAC'GTC
Cuts at: 0 228 603
Size: 228 375
Enzymes that do cut:
AatII AfeI AhdI AleI AvaI BanI BbeI BbvCI
BlpI Bme1580I BmtI BpmI Bpu10I BpuEI BsaHI BseRI
BsmBI Bsp1286I BsrBI BstYI BtsI EaeI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MscI MslI NarI
NheI NspI PstI SfcI SfoI SmaI SmlI StyI
XmaI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BmgBI BmrI BplI BsaI
BsaAI BsaBI BsaWI BsaXI BseYI BsgI BsiEI BsiHKAI
BsiWI BsmI BspEI BspHI BspMI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MspA1I
NaeI NcoI NdeI NgoMIV NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI