(Linear) (Six-base) MAPSORT of: KIBB8758.seq  Check: 4262  from: 1  to: 603



KIBB8758.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     384     603 
   Size:        384     219
 
AccI GT'mk_AC

Cuts at:      0     400     603 
   Size:        400     203
 
AflII C'TTAA_G

Cuts at:      0     291     603 
   Size:        291     312
 
AleI CACnn'nnGTG

Cuts at:      0     478     603 
   Size:        478     125
 
ApoI r'AATT_y

Cuts at:      0     127     310     603 
   Size:        127     183     293

  Fragments arranged by size: 

                 293     183     127
 
BseYI C'CCAG_C

Cuts at:      0     101     603 
   Size:        101     502
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     444     603 
   Size:        444     159
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     181     384     603 
   Size:        181     203     219

  Fragments arranged by size: 

                 219     203     181
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     469     603 
   Size:        469     134
 
BstBI TT'CG_AA

Cuts at:      0     507     603 
   Size:        507      96
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     480     603 
   Size:        480     123
 
Bsu36I CC'TnA_GG

Cuts at:      0     214     603 
   Size:        214     389
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     104     139     603 
   Size:        104      35     464

  Fragments arranged by size: 

                 464     104      35
 
DraI TTT'AAA

Cuts at:      0      24     134     603 
   Size:         24     110     469

  Fragments arranged by size: 

                 469     110      24
 
EarI CTCTTCn'nnn_

Cuts at:      0      52     112     504     603 
   Size:         52      60     392      99

  Fragments arranged by size: 

                 392      99      60      52
 
EcoRI G'AATT_C

Cuts at:      0     310     603 
   Size:        310     293
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     517     549     603 
   Size:        517      32      54

  Fragments arranged by size: 

                 517      54      32
 
HindIII A'AGCT_T

Cuts at:      0     279     416     603 
   Size:        279     137     187

  Fragments arranged by size: 

                 279     187     137
 
MslI CAynn'nnrTG

Cuts at:      0     478     603 
   Size:        478     125
 
SapI GCTCTTCn'nnn_

Cuts at:      0     112     603 
   Size:        112     491
 
SfcI C'TryA_G

Cuts at:      0     401     603 
   Size:        401     202
 
SmlI C'TyrA_G

Cuts at:      0     291     603 
   Size:        291     312
 
StyI C'CwwG_G

Cuts at:      0      36     603 
   Size:         36     567
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     266     298     603 
   Size:        266      32     305

  Fragments arranged by size: 

                 305     266      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     559     603 
   Size:        559      44
 
XmnI GAAnn'nnTTC

Cuts at:      0     310     503     603 
   Size:        310     193     100

  Fragments arranged by size: 

                 310     193     100
 

 Enzymes that do cut:

     AarI     AccI    AflII     AleI     ApoI    BseYI     BsgI    BspMI
   BstAPI    BstBI    BstXI   Bsu36I    CspCI     DraI     EarI    EcoRI
    Hin4I  HindIII     MslI     SapI     SfcI     SmlI     StyI     TstI
  Tth111I     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AcuI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstYI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TatI     XbaI     XcmI     XhoI
     XmaI     ZraI