(Linear) (Six-base) MAPSORT of: KIBB8759.seq  Check: 2190  from: 1  to: 603



KIBB8759.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     501     603 
   Size:        501     102
 
AccI GT'mk_AC

Cuts at:      0     377     603 
   Size:        377     226
 
AclI AA'CG_TT

Cuts at:      0     201     603 
   Size:        201     402
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     243     603 
   Size:        243     360
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     261     603 
   Size:        261     342
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     562     594     603 
   Size:        562      32       9

  Fragments arranged by size: 

                 562      32       9
 
AlwNI CAG_nnn'CTG

Cuts at:      0     516     603 
   Size:        516      87
 
ApoI r'AATT_y

Cuts at:      0      91     310     603 
   Size:         91     219     293

  Fragments arranged by size: 

                 293     219      91
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     464     497     603 
   Size:        464      33     106

  Fragments arranged by size: 

                 464     106      33
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     584     603 
   Size:        584      19
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     575     603 
   Size:        575      28
 
BglII A'GATC_T

Cuts at:      0     141     176     603 
   Size:        141      35     427

  Fragments arranged by size: 

                 427     141      35
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     166     556     603 
   Size:        157       9     390      47

  Fragments arranged by size: 

                 390     157      47       9
 
Bpu10I CC'TnA_GC

Cuts at:      0     341     603 
   Size:        341     262
 
BsaAI yAC'GTr

Cuts at:      0      81     603 
   Size:         81     522
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     562     592     603 
   Size:        562      30      11

  Fragments arranged by size: 

                 562      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     421     603 
   Size:        421     182
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     501     603 
   Size:        501     102
 
BsrDI GCAATG_nn'

Cuts at:      0     540     603 
   Size:        540      63
 
BstYI r'GATC_y

Cuts at:      0     141     176     603 
   Size:        141      35     427

  Fragments arranged by size: 

                 427     141      35
 
Bsu36I CC'TnA_GG

Cuts at:      0     214     603 
   Size:        214     389
 
BtsI GCAGTG_nn'

Cuts at:      0     185     603 
   Size:        185     418
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     157     166     243     556     603 
   Size:        157       9      77     313      47

  Fragments arranged by size: 

                 313     157      77      47       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     228     603 
   Size:        228     375
 
EcoO109I rG'GnC_Cy

Cuts at:      0     336     603 
   Size:        336     267
 
MspA1I CmG'CkG

Cuts at:      0     534     603 
   Size:        534      69
 
NspI r_CATG'y

Cuts at:      0     549     603 
   Size:        549      54
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     562     594     603 
   Size:        562      32       9

  Fragments arranged by size: 

                 562      32       9
 
PpuMI rG'GwC_Cy

Cuts at:      0     336     603 
   Size:        336     267
 
PstI C_TGCA'G

Cuts at:      0     192     603 
   Size:        192     411
 
PvuII CAG'CTG

Cuts at:      0     534     603 
   Size:        534      69
 
SexAI A'CCwGG_T

Cuts at:      0     418     603 
   Size:        418     185
 
SfcI C'TryA_G

Cuts at:      0     188     228     521     603 
   Size:        188      40     293      82

  Fragments arranged by size: 

                 293     188      82      40
 
SnaBI TAC'GTA

Cuts at:      0      81     603 
   Size:         81     522
 
SpeI A'CTAG_T

Cuts at:      0     457     603 
   Size:        457     146
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      28     603 
   Size:         28     575
 

 Enzymes that do cut:

     AarI     AccI     AclI     AcuI     AlfI     AloI    AlwNI     ApoI
     BaeI     BbsI     BcgI    BglII     BpmI   Bpu10I    BsaAI    BsaXI
    BseRI    BspMI    BsrDI    BstYI   Bsu36I     BtsI  Eco57MI    EcoNI
 EcoO109I   MspA1I     NspI     PpiI    PpuMI     PstI    PvuII    SexAI
     SfcI    SnaBI     SpeI    TaqII

 Enzymes that do not cut: 

    AatII   Acc65I     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI     BclI    BfrBI     BglI
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI    BpuEI     BsaI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BsrBI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I     BtgI
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI     SphI     SrfI     SspI     StuI     StyI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI