(Linear) (Six-base) MAPSORT of: KIBB8761.seq  Check: 9544  from: 1  to: 618



KIBB8761.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     344     618 
   Size:        254      90     274

  Fragments arranged by size: 

                 274     254      90
 
BamHI G'GATC_C

Cuts at:      0     528     618 
   Size:        528      90
 
BanII G_rGCy'C

Cuts at:      0     465     618 
   Size:        465     153
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     145     618 
   Size:        145     473
 
BglI GCCn_nnn'nGGC

Cuts at:      0     277     618 
   Size:        277     341
 
Bme1580I G_kGCm'C

Cuts at:      0     338     618 
   Size:        338     280
 
Bpu10I CC'TnA_GC

Cuts at:      0     464     475     618 
   Size:        464      11     143

  Fragments arranged by size: 

                 464     143      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     442     484     618 
   Size:        442      42     134

  Fragments arranged by size: 

                 442     134      42
 
BsmI GAATG_Cn'

Cuts at:      0     151     618 
   Size:        151     467
 
Bsp1286I G_dGCh'C

Cuts at:      0     338     465     618 
   Size:        338     127     153

  Fragments arranged by size: 

                 338     153     127
 
BsrGI T'GTAC_A

Cuts at:      0     522     618 
   Size:        522      96
 
BstYI r'GATC_y

Cuts at:      0     528     618 
   Size:        528      90
 
BtgI C'CryG_G

Cuts at:      0     298     618 
   Size:        298     320
 
BtsI GCAGTG_nn'

Cuts at:      0     429     618 
   Size:        429     189
 
DraIII CAC_nnn'GTG

Cuts at:      0     256     618 
   Size:        256     362
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     590     618 
   Size:        590      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     254     344     618 
   Size:        254      90     274

  Fragments arranged by size: 

                 274     254      90
 
HaeII r_GCGC'y

Cuts at:      0     318     618 
   Size:        318     300
 
MspA1I CmG'CkG

Cuts at:      0     300     618 
   Size:        300     318
 
NdeI CA'TA_TG

Cuts at:      0     612     618 
   Size:        612       6
 
SacII CC_GC'GG

Cuts at:      0     301     618 
   Size:        301     317
 
StyI C'CwwG_G

Cuts at:      0     383     541     618 
   Size:        383     158      77

  Fragments arranged by size: 

                 383     158      77
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     484     618 
   Size:        484     134
 
TatI w'GTAC_w

Cuts at:      0      85     522     618 
   Size:         85     437      96

  Fragments arranged by size: 

                 437      96      85
 

 Enzymes that do cut:

     AcuI    BamHI    BanII     BbsI     BglI Bme1580I   Bpu10I    BseRI
     BsmI Bsp1286I    BsrGI    BstYI     BtgI     BtsI   DraIII     EciI
  Eco57MI    HaeII   MspA1I     NdeI    SacII     StyI    TaqII     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI     EagI     EarI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI