Restriction map for
pF1KB8762
(Linear) (Six-base) MAPSORT of: KIBB8762.seq Check: 8163 from: 1 to: 630
KIBB8762.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 239 630
Size: 46 193 391
Fragments arranged by size:
391 193 46
AflIII A'CryG_T
Cuts at: 0 149 630
Size: 149 481
AhdI GACnn_n'nnGTC
Cuts at: 0 72 630
Size: 72 558
AleI CACnn'nnGTG
Cuts at: 0 10 630
Size: 10 620
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 307 339 630
Size: 307 32 291
Fragments arranged by size:
307 291 32
AlwNI CAG_nnn'CTG
Cuts at: 0 466 606 630
Size: 466 140 24
Fragments arranged by size:
466 140 24
BanI G'GyrC_C
Cuts at: 0 12 229 630
Size: 12 217 401
Fragments arranged by size:
401 217 12
BanII G_rGCy'C
Cuts at: 0 287 515 630
Size: 287 228 115
Fragments arranged by size:
287 228 115
BbvCI CC'TCA_GC
Cuts at: 0 413 630
Size: 413 217
BclI T'GATC_A
Cuts at: 0 86 630
Size: 86 544
Bme1580I G_kGCm'C
Cuts at: 0 17 630
Size: 17 613
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 486 630
Size: 486 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 424 471 630
Size: 424 47 159
Fragments arranged by size:
424 159 47
Bpu10I CC'TnA_GC
Cuts at: 0 413 630
Size: 413 217
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 630
Size: 295 335
BsaI GGTCTCn'nnnn_
Cuts at: 0 400 630
Size: 400 230
BsaAI yAC'GTr
Cuts at: 0 552 630
Size: 552 78
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 307 337 425 455 630
Size: 307 30 88 30 175
Fragments arranged by size:
307 175 88 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 523 630
Size: 523 107
BseYI C'CCAG_C
Cuts at: 0 99 630
Size: 99 531
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 382 532 630
Size: 382 150 98
Fragments arranged by size:
382 150 98
BsiHKAI G_wGCw'C
Cuts at: 0 287 630
Size: 287 343
Bsp1286I G_dGCh'C
Cuts at: 0 17 287 515 630
Size: 17 270 228 115
Fragments arranged by size:
270 228 115 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 77 630
Size: 77 553
BstYI r'GATC_y
Cuts at: 0 620 630
Size: 620 10
BtgI C'CryG_G
Cuts at: 0 203 630
Size: 203 427
BtsI GCAGTG_nn'
Cuts at: 0 138 150 630
Size: 138 12 480
Fragments arranged by size:
480 138 12
DraIII CAC_nnn'GTG
Cuts at: 0 272 630
Size: 272 358
DrdI GACnn_nn'nnGTC
Cuts at: 0 495 630
Size: 495 135
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 100 630
Size: 100 530
EcoICRI GAG'CTC
Cuts at: 0 285 630
Size: 285 345
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 239 424 471 630
Size: 46 193 185 47 159
Fragments arranged by size:
193 185 159 47 46
EcoO109I rG'GnC_Cy
Cuts at: 0 66 214 562 603 630
Size: 66 148 348 41 27
Fragments arranged by size:
348 148 66 41 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 58 90 283 315 582 614 630
Size: 58 32 193 32 267 32 16
Fragments arranged by size:
267 193 58 32 32 32 16
HindIII A'AGCT_T
Cuts at: 0 58 630
Size: 58 572
MslI CAynn'nnrTG
Cuts at: 0 10 630
Size: 10 620
MspA1I CmG'CkG
Cuts at: 0 99 630
Size: 99 531
NcoI C'CATG_G
Cuts at: 0 203 630
Size: 203 427
NspI r_CATG'y
Cuts at: 0 153 618 630
Size: 153 465 12
Fragments arranged by size:
465 153 12
PciI A'CATG_T
Cuts at: 0 149 630
Size: 149 481
PflMI CCAn_nnn'nTGG
Cuts at: 0 466 630
Size: 466 164
PfoI T'CCnGG_A
Cuts at: 0 287 572 630
Size: 287 285 58
Fragments arranged by size:
287 285 58
PmlI CAC'GTG
Cuts at: 0 552 630
Size: 552 78
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 307 339 630
Size: 307 32 291
Fragments arranged by size:
307 291 32
PpuMI rG'GwC_Cy
Cuts at: 0 66 603 630
Size: 66 537 27
Fragments arranged by size:
537 66 27
PstI C_TGCA'G
Cuts at: 0 176 590 630
Size: 176 414 40
Fragments arranged by size:
414 176 40
SacI G_AGCT'C
Cuts at: 0 287 630
Size: 287 343
SbfI CC_TGCA'GG
Cuts at: 0 176 630
Size: 176 454
SfcI C'TryA_G
Cuts at: 0 172 586 630
Size: 172 414 44
Fragments arranged by size:
414 172 44
SmlI C'TyrA_G
Cuts at: 0 310 630
Size: 310 320
SphI G_CATG'C
Cuts at: 0 618 630
Size: 618 12
StyI C'CwwG_G
Cuts at: 0 8 203 630
Size: 8 195 427
Fragments arranged by size:
427 195 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 396 630
Size: 396 234
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 630
Size: 425 32 173
Fragments arranged by size:
425 173 32
Enzymes that do cut:
AcuI AflIII AhdI AleI AloI AlwNI BanI BanII
BbvCI BclI Bme1580I BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BstYI BtgI BtsI DraIII DrdI EciI EcoICRI Eco57MI
EcoO109I Hin4I HindIII MslI MspA1I NcoI NspI PciI
PflMI PfoI PmlI PpiI PpuMI PstI SacI SbfI
SfcI SmlI SphI StyI TaqII TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AlfI ApaI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BsaBI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI EaeI
EagI EarI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TatI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI