Restriction map for
pF1KB8764
(Linear) (Six-base) MAPSORT of: KIBB8764.seq Check: 9413 from: 1 to: 654
KIBB8764.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 238 240 423 654
Size: 238 2 183 231
Fragments arranged by size:
238 231 183 2
ApaI G_GGCC'C
Cuts at: 0 386 654
Size: 386 268
BamHI G'GATC_C
Cuts at: 0 366 654
Size: 366 288
BanI G'GyrC_C
Cuts at: 0 151 206 280 439 654
Size: 151 55 74 159 215
Fragments arranged by size:
215 159 151 74 55
BanII G_rGCy'C
Cuts at: 0 386 540 654
Size: 386 154 114
Fragments arranged by size:
386 154 114
BbsI GAAGACnn'nnnn_
Cuts at: 0 20 654
Size: 20 634
BglI GCCn_nnn'nGGC
Cuts at: 0 242 381 654
Size: 242 139 273
Fragments arranged by size:
273 242 139
Bme1580I G_kGCm'C
Cuts at: 0 154 209 283 386 442 654
Size: 154 55 74 103 56 212
Fragments arranged by size:
212 154 103 74 56 55
BmrI ACTGGGnnnn_n'
Cuts at: 0 423 654
Size: 423 231
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 448 562 586 654
Size: 349 99 114 24 68
Fragments arranged by size:
349 114 99 68 24
Bpu10I CC'TnA_GC
Cuts at: 0 481 654
Size: 481 173
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 86 116 559 589 654
Size: 86 30 443 30 65
Fragments arranged by size:
443 86 65 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 85 337 559 654
Size: 40 45 252 222 95
Fragments arranged by size:
252 222 95 45 40
BseYI C'CCAG_C
Cuts at: 0 202 352 386 654
Size: 202 150 34 268
Fragments arranged by size:
268 202 150 34
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 654
Size: 272 382
Bsp1286I G_dGCh'C
Cuts at: 0 154 209 283 386 442 540 654
Size: 154 55 74 103 56 98 114
Fragments arranged by size:
154 114 103 98 74 56 55
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 238 240 423 654
Size: 238 2 183 231
Fragments arranged by size:
238 231 183 2
BssSI C'ACGA_G
Cuts at: 0 197 654
Size: 197 457
BstYI r'GATC_y
Cuts at: 0 366 654
Size: 366 288
BtgI C'CryG_G
Cuts at: 0 258 342 378 443 654
Size: 258 84 36 65 211
Fragments arranged by size:
258 211 84 65 36
BtsI GCAGTG_nn'
Cuts at: 0 284 503 654
Size: 284 219 151
Fragments arranged by size:
284 219 151
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 384 419 654
Size: 384 35 235
Fragments arranged by size:
384 235 35
EaeI y'GGCC_r
Cuts at: 0 339 654
Size: 339 315
EarI CTCTTCn'nnn_
Cuts at: 0 17 71 328 654
Size: 17 54 257 326
Fragments arranged by size:
326 257 54 17
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 349 448 562 586 654
Size: 349 99 114 24 68
Fragments arranged by size:
349 114 99 68 24
EcoNI CCTnn'n_nnAGG
Cuts at: 0 430 544 654
Size: 430 114 110
Fragments arranged by size:
430 114 110
EcoO109I rG'GnC_Cy
Cuts at: 0 374 383 654
Size: 374 9 271
Fragments arranged by size:
374 271 9
HaeII r_GCGC'y
Cuts at: 0 363 654
Size: 363 291
HincII GTy'rAC
Cuts at: 0 273 654
Size: 273 381
MscI TGG'CCA
Cuts at: 0 341 654
Size: 341 313
MslI CAynn'nnrTG
Cuts at: 0 133 654
Size: 133 521
MspA1I CmG'CkG
Cuts at: 0 609 654
Size: 609 45
NcoI C'CATG_G
Cuts at: 0 342 443 654
Size: 342 101 211
Fragments arranged by size:
342 211 101
NspI r_CATG'y
Cuts at: 0 315 605 654
Size: 315 290 49
Fragments arranged by size:
315 290 49
PasI CC'CwG_GG
Cuts at: 0 265 654
Size: 265 389
PfoI T'CCnGG_A
Cuts at: 0 425 563 654
Size: 425 138 91
Fragments arranged by size:
425 138 91
PspOMI G'GGCC_C
Cuts at: 0 382 654
Size: 382 272
PstI C_TGCA'G
Cuts at: 0 630 654
Size: 630 24
SfcI C'TryA_G
Cuts at: 0 626 654
Size: 626 28
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 381 654
Size: 381 273
SphI G_CATG'C
Cuts at: 0 315 605 654
Size: 315 290 49
Fragments arranged by size:
315 290 49
SspI AAT'ATT
Cuts at: 0 178 654
Size: 178 476
StyI C'CwwG_G
Cuts at: 0 342 370 443 654
Size: 342 28 73 211
Fragments arranged by size:
342 211 73 28
TatI w'GTAC_w
Cuts at: 0 633 654
Size: 633 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 559 591 654
Size: 559 32 63
Fragments arranged by size:
559 63 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 349 654
Size: 349 305
Enzymes that do cut:
AarI ApaI BamHI BanI BanII BbsI BglI Bme1580I
BmrI BpmI Bpu10I BsaXI BseRI BseYI BsgI Bsp1286I
BspMI BssSI BstYI BtgI BtsI CspCI EaeI EarI
Eco57MI EcoNI EcoO109I HaeII HincII MscI MslI MspA1I
NcoI NspI PasI PfoI PspOMI PstI SfcI SfiI
SphI SspI StyI TatI TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsiEI
BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtgZI ClaI DraI DraIII DrdI EagI EciI
EcoICRI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI StuI SwaI TaqII
Tth111I XbaI XhoI XmaI XmnI ZraI