Restriction map for
pF1KB8766
(Linear) (Six-base) MAPSORT of: KIBB8766.seq Check: 4167 from: 1 to: 675
KIBB8766.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 294 675
Size: 294 381
AccI GT'mk_AC
Cuts at: 0 4 675
Size: 4 671
Acc65I G'GTAC_C
Cuts at: 0 55 675
Size: 55 620
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 675
Size: 242 433
ApaI G_GGCC'C
Cuts at: 0 518 675
Size: 518 157
ApoI r'AATT_y
Cuts at: 0 634 675
Size: 634 41
BanI G'GyrC_C
Cuts at: 0 55 675
Size: 55 620
BanII G_rGCy'C
Cuts at: 0 79 362 518 675
Size: 79 283 156 157
Fragments arranged by size:
283 157 156 79
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 292 326 675
Size: 292 34 349
Fragments arranged by size:
349 292 34
BfrBI ATG'CAT
Cuts at: 0 448 675
Size: 448 227
BglII A'GATC_T
Cuts at: 0 290 675
Size: 290 385
BlpI GC'TnA_GC
Cuts at: 0 105 675
Size: 105 570
Bme1580I G_kGCm'C
Cuts at: 0 206 518 675
Size: 206 312 157
Fragments arranged by size:
312 206 157
BmrI ACTGGGnnnn_n'
Cuts at: 0 203 675
Size: 203 472
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 114 675
Size: 114 561
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 376 580 675
Size: 295 81 204 95
Fragments arranged by size:
295 204 95 81
BsaAI yAC'GTr
Cuts at: 0 379 675
Size: 379 296
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 675
Size: 451 224
BseYI C'CCAG_C
Cuts at: 0 154 675
Size: 154 521
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 675
Size: 401 274
BsiEI CG_ry'CG
Cuts at: 0 150 675
Size: 150 525
BsiHKAI G_wGCw'C
Cuts at: 0 362 675
Size: 362 313
Bsp1286I G_dGCh'C
Cuts at: 0 79 206 362 518 675
Size: 79 127 156 156 157
Fragments arranged by size:
157 156 156 127 79
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 194 294 675
Size: 194 100 381
Fragments arranged by size:
381 194 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 324 675
Size: 324 351
BstXI CCAn_nnnn'nTGG
Cuts at: 0 51 675
Size: 51 624
BstYI r'GATC_y
Cuts at: 0 290 675
Size: 290 385
Bsu36I CC'TnA_GG
Cuts at: 0 61 675
Size: 61 614
BtgI C'CryG_G
Cuts at: 0 119 675
Size: 119 556
BtsI GCAGTG_nn'
Cuts at: 0 552 675
Size: 552 123
DraIII CAC_nnn'GTG
Cuts at: 0 671 675
Size: 671 4
EcoICRI GAG'CTC
Cuts at: 0 360 675
Size: 360 315
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 242 295 376 580 675
Size: 242 53 81 204 95
Fragments arranged by size:
242 204 95 81 53
EcoO109I rG'GnC_Cy
Cuts at: 0 462 515 675
Size: 462 53 160
Fragments arranged by size:
462 160 53
EcoRI G'AATT_C
Cuts at: 0 634 675
Size: 634 41
FspI TGC'GCA
Cuts at: 0 190 675
Size: 190 485
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 292 324 675
Size: 292 32 351
Fragments arranged by size:
351 292 32
KpnI G_GTAC'C
Cuts at: 0 59 675
Size: 59 616
NsiI A_TGCA'T
Cuts at: 0 450 675
Size: 450 225
NspI r_CATG'y
Cuts at: 0 285 675
Size: 285 390
PasI CC'CwG_GG
Cuts at: 0 51 579 675
Size: 51 528 96
Fragments arranged by size:
528 96 51
PfoI T'CCnGG_A
Cuts at: 0 176 675
Size: 176 499
PmlI CAC'GTG
Cuts at: 0 379 675
Size: 379 296
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 226 258 675
Size: 226 32 417
Fragments arranged by size:
417 226 32
PpuMI rG'GwC_Cy
Cuts at: 0 462 675
Size: 462 213
PspOMI G'GGCC_C
Cuts at: 0 514 675
Size: 514 161
PstI C_TGCA'G
Cuts at: 0 479 675
Size: 479 196
SacI G_AGCT'C
Cuts at: 0 362 675
Size: 362 313
SexAI A'CCwGG_T
Cuts at: 0 667 675
Size: 667 8
SfcI C'TryA_G
Cuts at: 0 342 475 675
Size: 342 133 200
Fragments arranged by size:
342 200 133
StuI AGG'CCT
Cuts at: 0 115 675
Size: 115 560
StyI C'CwwG_G
Cuts at: 0 110 675
Size: 110 565
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 200 232 675
Size: 200 32 443
Fragments arranged by size:
443 200 32
XmnI GAAnn'nnTTC
Cuts at: 0 490 675
Size: 490 185
Enzymes that do cut:
AarI AccI Acc65I AcuI ApaI ApoI BanI BanII
BcgI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI
BsaAI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI
BstAPI BstXI BstYI Bsu36I BtgI BtsI DraIII EcoICRI
Eco57MI EcoO109I EcoRI FspI Hin4I KpnI NsiI NspI
PasI PfoI PmlI PpiI PpuMI PspOMI PstI SacI
SexAI SfcI StuI StyI TstI XmnI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI
BglI BmgBI BmtI Bpu10I BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I
BtgZI ClaI CspCI DraI DrdI EaeI EagI EarI
EciI EcoNI EcoRV FalI FseI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MmeI MscI MslI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI PacI PciI PflMI PmeI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI SwaI TaqII TatI
Tth111I XbaI XcmI XhoI XmaI ZraI