(Linear) (Six-base) MAPSORT of: KIBB8768.seq  Check: 7163  from: 1  to: 687



KIBB8768.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     344     687 
   Size:        344     343
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     400     687 
   Size:         61     339     287

  Fragments arranged by size: 

                 339     287      61
 
AlwNI CAG_nnn'CTG

Cuts at:      0      36     231     570     687 
   Size:         36     195     339     117

  Fragments arranged by size: 

                 339     195     117      36
 
AvaI C'yCGr_G

Cuts at:      0     320     625     687 
   Size:        320     305      62

  Fragments arranged by size: 

                 320     305      62
 
BanI G'GyrC_C

Cuts at:      0     130     496     687 
   Size:        130     366     191

  Fragments arranged by size: 

                 366     191     130
 
BanII G_rGCy'C

Cuts at:      0     626     687 
   Size:        626      61
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     687 
   Size:        601      86
 
BseYI C'CCAG_C

Cuts at:      0     231     687 
   Size:        231     456
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     244     583     687 
   Size:        145      99     339     104

  Fragments arranged by size: 

                 339     145     104      99
 
Bsp1286I G_dGCh'C

Cuts at:      0     626     687 
   Size:        626      61
 
BspHI T'CATG_A

Cuts at:      0     189     687 
   Size:        189     498
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     206     431     687 
   Size:        206     225     256

  Fragments arranged by size: 

                 256     225     206
 
BsrBI CCG'CTC

Cuts at:      0     176     687 
   Size:        176     511
 
BsrFI r'CCGG_y

Cuts at:      0     610     687 
   Size:        610      77
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     444     687 
   Size:        444     243
 
BtgI C'CryG_G

Cuts at:      0     243     687 
   Size:        243     444
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     523     687 
   Size:        523     164
 
DraIII CAC_nnn'GTG

Cuts at:      0     249     687 
   Size:        249     438
 
EarI CTCTTCn'nnn_

Cuts at:      0     368     687 
   Size:        368     319
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      61     400     601     687 
   Size:         61     339     201      86

  Fragments arranged by size: 

                 339     201      86      61
 
HaeII r_GCGC'y

Cuts at:      0     309     687 
   Size:        309     378
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     619     651     687 
   Size:        619      32      36

  Fragments arranged by size: 

                 619      36      32
 
MslI CAynn'nnrTG

Cuts at:      0     682     687 
   Size:        682       5
 
MspA1I CmG'CkG

Cuts at:      0     136     687 
   Size:        136     551
 
NspI r_CATG'y

Cuts at:      0     582     687 
   Size:        582     105
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     231     687 
   Size:        231     456
 
PfoI T'CCnGG_A

Cuts at:      0     617     687 
   Size:        617      70
 
PstI C_TGCA'G

Cuts at:      0     241     305     687 
   Size:        241      64     382

  Fragments arranged by size: 

                 382     241      64
 
SapI GCTCTTCn'nnn_

Cuts at:      0     368     687 
   Size:        368     319
 
SfcI C'TryA_G

Cuts at:      0     237     301     687 
   Size:        237      64     386

  Fragments arranged by size: 

                 386     237      64
 
SmaI CCC'GGG

Cuts at:      0     322     687 
   Size:        322     365
 
StuI AGG'CCT

Cuts at:      0     385     687 
   Size:        385     302
 
StyI C'CwwG_G

Cuts at:      0      23     687 
   Size:         23     664
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     484     565     687 
   Size:        484      81     122

  Fragments arranged by size: 

                 484     122      81
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     444     507     687 
   Size:        444      63     180

  Fragments arranged by size: 

                 444     180      63
 
XmaI C'CCGG_G

Cuts at:      0     320     687 
   Size:        320     367
 
XmnI GAAnn'nnTTC

Cuts at:      0     385     687 
   Size:        385     302
 

 Enzymes that do cut:

     AccI     AcuI    AlwNI     AvaI     BanI    BanII     BpmI    BseYI
     BsgI Bsp1286I    BspHI    BspMI    BsrBI    BsrFI    BstXI     BtgI
    BtgZI   DraIII     EarI  Eco57MI    HaeII    Hin4I     MslI   MspA1I
     NspI    PflMI     PfoI     PstI     SapI     SfcI     SmaI     StuI
     StyI    TaqII     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI    BspEI    BsrDI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstYI  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     ZraI