Restriction map for
pF1KB8769
(Linear) (Six-base) MAPSORT of: KIBB8769.seq Check: 8820 from: 1 to: 699
KIBB8769.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 433 699
Size: 177 256 266
Fragments arranged by size:
266 256 177
AfeI AGC'GCT
Cuts at: 0 670 699
Size: 670 29
AlwNI CAG_nnn'CTG
Cuts at: 0 249 699
Size: 249 450
ApaI G_GGCC'C
Cuts at: 0 647 699
Size: 647 52
ApoI r'AATT_y
Cuts at: 0 167 699
Size: 167 532
AvaI C'yCGr_G
Cuts at: 0 686 699
Size: 686 13
BanII G_rGCy'C
Cuts at: 0 507 647 699
Size: 507 140 52
Fragments arranged by size:
507 140 52
BglI GCCn_nnn'nGGC
Cuts at: 0 18 699
Size: 18 681
Bme1580I G_kGCm'C
Cuts at: 0 647 699
Size: 647 52
BmrI ACTGGGnnnn_n'
Cuts at: 0 95 699
Size: 95 604
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 699
Size: 532 167
BsaI GGTCTCn'nnnn_
Cuts at: 0 264 428 699
Size: 264 164 271
Fragments arranged by size:
271 264 164
BsaWI w'CCGG_w
Cuts at: 0 77 437 699
Size: 77 360 262
Fragments arranged by size:
360 262 77
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 301 699
Size: 280 21 398
Fragments arranged by size:
398 280 21
BsmI GAATG_Cn'
Cuts at: 0 570 699
Size: 570 129
Bsp1286I G_dGCh'C
Cuts at: 0 507 647 699
Size: 507 140 52
Fragments arranged by size:
507 140 52
BsrDI GCAATG_nn'
Cuts at: 0 614 699
Size: 614 85
BstAPI GCAn_nnn'nTGC
Cuts at: 0 132 672 699
Size: 132 540 27
Fragments arranged by size:
540 132 27
BstYI r'GATC_y
Cuts at: 0 224 245 699
Size: 224 21 454
Fragments arranged by size:
454 224 21
EaeI y'GGCC_r
Cuts at: 0 2 609 699
Size: 2 607 90
Fragments arranged by size:
607 90 2
EarI CTCTTCn'nnn_
Cuts at: 0 152 194 257 278 344 554 575 699
Size: 152 42 63 21 66 210 21 124
Fragments arranged by size:
210 152 124 66 63 42 21 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 177 433 699
Size: 177 256 266
Fragments arranged by size:
266 256 177
EcoNI CCTnn'n_nnAGG
Cuts at: 0 448 469 511 553 574 699
Size: 448 21 42 42 21 125
Fragments arranged by size:
448 125 42 42 21 21
EcoO109I rG'GnC_Cy
Cuts at: 0 462 483 587 644 699
Size: 462 21 104 57 55
Fragments arranged by size:
462 104 57 55 21
HaeII r_GCGC'y
Cuts at: 0 672 699
Size: 672 27
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 209 241 298 330 529 561 699
Size: 209 32 57 32 199 32 138
Fragments arranged by size:
209 199 138 57 32 32 32
MscI TGG'CCA
Cuts at: 0 4 611 699
Size: 4 607 88
Fragments arranged by size:
607 88 4
NruI TCG'CGA
Cuts at: 0 155 699
Size: 155 544
PflMI CCAn_nnn'nTGG
Cuts at: 0 249 699
Size: 249 450
PspOMI G'GGCC_C
Cuts at: 0 643 699
Size: 643 56
PstI C_TGCA'G
Cuts at: 0 137 699
Size: 137 562
SfcI C'TryA_G
Cuts at: 0 133 699
Size: 133 566
SmlI C'TyrA_G
Cuts at: 0 511 699
Size: 511 188
StuI AGG'CCT
Cuts at: 0 400 699
Size: 400 299
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 190 222 699
Size: 190 32 477
Fragments arranged by size:
477 190 32
XmnI GAAnn'nnTTC
Cuts at: 0 295 358 699
Size: 295 63 341
Fragments arranged by size:
341 295 63
Enzymes that do cut:
AcuI AfeI AlwNI ApaI ApoI AvaI BanII BglI
Bme1580I BmrI BpuEI BsaI BsaWI BseRI BsmI Bsp1286I
BsrDI BstAPI BstYI EaeI EarI Eco57MI EcoNI EcoO109I
HaeII Hin4I MscI NruI PflMI PspOMI PstI SfcI
SmlI StuI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaXI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI MspA1I NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmaI ZraI