Restriction map for
pF1KB8770
(Linear) (Six-base) MAPSORT of: KIBB8770.seq Check: 5355 from: 1 to: 702
KIBB8770.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AflII C'TTAA_G
Cuts at: 0 442 702
Size: 442 260
AflIII A'CryG_T
Cuts at: 0 692 702
Size: 692 10
ApaLI G'TGCA_C
Cuts at: 0 252 702
Size: 252 450
ApoI r'AATT_y
Cuts at: 0 271 702
Size: 271 431
AvaI C'yCGr_G
Cuts at: 0 160 583 702
Size: 160 423 119
Fragments arranged by size:
423 160 119
BanII G_rGCy'C
Cuts at: 0 658 702
Size: 658 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 628 702
Size: 628 74
BbvCI CC'TCA_GC
Cuts at: 0 676 702
Size: 676 26
BglII A'GATC_T
Cuts at: 0 5 702
Size: 5 697
Bme1580I G_kGCm'C
Cuts at: 0 256 702
Size: 256 446
BmgBI CAC'GTC
Cuts at: 0 695 702
Size: 695 7
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 252 702
Size: 252 450
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 702
Size: 652 50
Bpu10I CC'TnA_GC
Cuts at: 0 47 676 702
Size: 47 629 26
Fragments arranged by size:
629 47 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 226 301 702
Size: 226 75 401
Fragments arranged by size:
401 226 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 111 526 702
Size: 111 415 176
Fragments arranged by size:
415 176 111
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 564 594 702
Size: 564 30 108
Fragments arranged by size:
564 108 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 241 702
Size: 241 461
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 435 648 702
Size: 435 213 54
Fragments arranged by size:
435 213 54
BsiHKAI G_wGCw'C
Cuts at: 0 56 256 518 658 702
Size: 56 200 262 140 44
Fragments arranged by size:
262 200 140 56 44
Bsp1286I G_dGCh'C
Cuts at: 0 56 256 518 658 702
Size: 56 200 262 140 44
Fragments arranged by size:
262 200 140 56 44
BsrFI r'CCGG_y
Cuts at: 0 289 702
Size: 289 413
BstYI r'GATC_y
Cuts at: 0 5 702
Size: 5 697
BtgI C'CryG_G
Cuts at: 0 566 702
Size: 566 136
BtsI GCAGTG_nn'
Cuts at: 0 560 702
Size: 560 142
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 447 482 702
Size: 447 35 220
Fragments arranged by size:
447 220 35
EaeI y'GGCC_r
Cuts at: 0 569 702
Size: 569 133
EcoICRI GAG'CTC
Cuts at: 0 656 702
Size: 656 46
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 652 702
Size: 652 50
EcoNI CCTnn'n_nnAGG
Cuts at: 0 61 702
Size: 61 641
EcoO109I rG'GnC_Cy
Cuts at: 0 597 702
Size: 597 105
MfeI C'AATT_G
Cuts at: 0 259 702
Size: 259 443
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 702
Size: 322 380
MscI TGG'CCA
Cuts at: 0 571 702
Size: 571 131
MspA1I CmG'CkG
Cuts at: 0 397 702
Size: 397 305
NaeI GCC'GGC
Cuts at: 0 291 702
Size: 291 411
NcoI C'CATG_G
Cuts at: 0 566 702
Size: 566 136
NgoMIV G'CCGG_C
Cuts at: 0 289 702
Size: 289 413
PasI CC'CwG_GG
Cuts at: 0 509 702
Size: 509 193
PflMI CCAn_nnn'nTGG
Cuts at: 0 567 702
Size: 567 135
PpuMI rG'GwC_Cy
Cuts at: 0 597 702
Size: 597 105
PstI C_TGCA'G
Cuts at: 0 205 702
Size: 205 497
PvuII CAG'CTG
Cuts at: 0 397 702
Size: 397 305
SacI G_AGCT'C
Cuts at: 0 658 702
Size: 658 44
SfcI C'TryA_G
Cuts at: 0 9 201 378 702
Size: 9 192 177 324
Fragments arranged by size:
324 192 177 9
SmaI CCC'GGG
Cuts at: 0 162 702
Size: 162 540
SmlI C'TyrA_G
Cuts at: 0 241 316 442 702
Size: 241 75 126 260
Fragments arranged by size:
260 241 126 75
StyI C'CwwG_G
Cuts at: 0 566 702
Size: 566 136
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 151 702
Size: 151 551
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 564 596 702
Size: 564 32 106
Fragments arranged by size:
564 106 32
XmaI C'CCGG_G
Cuts at: 0 160 702
Size: 160 542
Enzymes that do cut:
AflII AflIII ApaLI ApoI AvaI BanII BbsI BbvCI
BglII Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI
BsaXI BseRI BsgI BsiHKAI Bsp1286I BsrFI BstYI BtgI
BtsI CspCI EaeI EcoICRI Eco57MI EcoNI EcoO109I MfeI
MmeI MscI MspA1I NaeI NcoI NgoMIV PasI PflMI
PpuMI PstI PvuII SacI SfcI SmaI SmlI StyI
TaqII TstI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BcgI BciVI
BclI BfrBI BglI BlpI BmrI BmtI BsaAI BsaBI
BsaHI BsaWI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EagI EarI EciI EcoRI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MluI MslI NarI NdeI NheI NotI
NruI NsiI NspI PacI PciI PfoI PmeI PmlI
PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StuI SwaI TatI Tth111I XbaI XcmI XhoI XmnI
ZraI