Restriction map for
pF1KB8771
(Linear) (Six-base) MAPSORT of: KIBB8771.seq Check: 4546 from: 1 to: 702
KIBB8771.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 211 213 339 702
Size: 211 2 126 363
Fragments arranged by size:
363 211 126 2
ApaI G_GGCC'C
Cuts at: 0 627 702
Size: 627 75
AscI GG'CGCG_CC
Cuts at: 0 388 541 702
Size: 388 153 161
Fragments arranged by size:
388 161 153
AvaI C'yCGr_G
Cuts at: 0 175 237 373 658 702
Size: 175 62 136 285 44
Fragments arranged by size:
285 175 136 62 44
BanI G'GyrC_C
Cuts at: 0 93 126 162 170 272 313 610 702
Size: 93 33 36 8 102 41 297 92
Fragments arranged by size:
297 102 93 92 41 36 33 8
BanII G_rGCy'C
Cuts at: 0 325 627 702
Size: 325 302 75
Fragments arranged by size:
325 302 75
BbeI G_GCGC'C
Cuts at: 0 97 130 166 174 317 614 702
Size: 97 33 36 8 143 297 88
Fragments arranged by size:
297 143 97 88 36 33 8
BglI GCCn_nnn'nGGC
Cuts at: 0 608 702
Size: 608 94
Bme1580I G_kGCm'C
Cuts at: 0 627 702
Size: 627 75
BmrI ACTGGGnnnn_n'
Cuts at: 0 94 204 393 702
Size: 94 110 189 309
Fragments arranged by size:
309 189 110 94
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 469 511 702
Size: 322 147 42 191
Fragments arranged by size:
322 191 147 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 631 702
Size: 631 71
BsaHI Gr'CG_yC
Cuts at: 0 94 100 106 127 163 171 314 611
Size: 94 6 6 21 36 8 143 297
Cuts at: 611 702
Size: 91
Fragments arranged by size:
297 143 94 91 36 21 8 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 18 294 702
Size: 18 276 408
Fragments arranged by size:
408 276 18
BseYI C'CCAG_C
Cuts at: 0 620 702
Size: 620 82
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 6 702
Size: 6 696
BsiEI CG_ry'CG
Cuts at: 0 117 702
Size: 117 585
BsiHKAI G_wGCw'C
Cuts at: 0 325 702
Size: 325 377
Bsp1286I G_dGCh'C
Cuts at: 0 325 627 702
Size: 325 302 75
Fragments arranged by size:
325 302 75
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 211 213 339 702
Size: 211 2 126 363
Fragments arranged by size:
363 211 126 2
BsrBI CCG'CTC
Cuts at: 0 441 453 495 564 645 702
Size: 441 12 42 69 81 57
Fragments arranged by size:
441 81 69 57 42 12
BsrFI r'CCGG_y
Cuts at: 0 15 90 290 431 702
Size: 15 75 200 141 271
Fragments arranged by size:
271 200 141 75 15
BssHII G'CGCG_C
Cuts at: 0 284 388 410 483 525 541 702
Size: 284 104 22 73 42 16 161
Fragments arranged by size:
284 161 104 73 42 22 16
Bsu36I CC'TnA_GG
Cuts at: 0 277 702
Size: 277 425
BtgI C'CryG_G
Cuts at: 0 26 39 231 467 530 702
Size: 26 13 192 236 63 172
Fragments arranged by size:
236 192 172 63 26 13
DrdI GACnn_nn'nnGTC
Cuts at: 0 679 702
Size: 679 23
EaeI y'GGCC_r
Cuts at: 0 114 416 464 702
Size: 114 302 48 238
Fragments arranged by size:
302 238 114 48
EagI C'GGCC_G
Cuts at: 0 114 702
Size: 114 588
EarI CTCTTCn'nnn_
Cuts at: 0 515 702
Size: 515 187
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 516 702
Size: 516 186
EcoICRI GAG'CTC
Cuts at: 0 323 702
Size: 323 379
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 469 511 702
Size: 322 147 42 191
Fragments arranged by size:
322 191 147 42
EcoO109I rG'GnC_Cy
Cuts at: 0 254 624 693 702
Size: 254 370 69 9
Fragments arranged by size:
370 254 69 9
HaeII r_GCGC'y
Cuts at: 0 97 130 166 174 317 511 574 614
Size: 97 33 36 8 143 194 63 40
Cuts at: 614 702
Size: 88
Fragments arranged by size:
194 143 97 88 63 40 36 33
8
KasI G'GCGC_C
Cuts at: 0 93 126 162 170 313 610 702
Size: 93 33 36 8 143 297 92
Fragments arranged by size:
297 143 93 92 36 33 8
MspA1I CmG'CkG
Cuts at: 0 41 233 311 364 532 631 702
Size: 41 192 78 53 168 99 71
Fragments arranged by size:
192 168 99 78 71 53 41
NaeI GCC'GGC
Cuts at: 0 17 92 433 702
Size: 17 75 341 269
Fragments arranged by size:
341 269 75 17
NarI GG'CG_CC
Cuts at: 0 94 127 163 171 314 611 702
Size: 94 33 36 8 143 297 91
Fragments arranged by size:
297 143 94 91 36 33 8
NcoI C'CATG_G
Cuts at: 0 26 702
Size: 26 676
NgoMIV G'CCGG_C
Cuts at: 0 15 90 431 702
Size: 15 75 341 271
Fragments arranged by size:
341 271 75 15
PasI CC'CwG_GG
Cuts at: 0 615 702
Size: 615 87
PfoI T'CCnGG_A
Cuts at: 0 78 702
Size: 78 624
PpuMI rG'GwC_Cy
Cuts at: 0 254 693 702
Size: 254 439 9
Fragments arranged by size:
439 254 9
PspOMI G'GGCC_C
Cuts at: 0 623 702
Size: 623 79
PstI C_TGCA'G
Cuts at: 0 515 557 578 702
Size: 515 42 21 124
Fragments arranged by size:
515 124 42 21
SacI G_AGCT'C
Cuts at: 0 325 702
Size: 325 377
SacII CC_GC'GG
Cuts at: 0 42 234 533 702
Size: 42 192 299 169
Fragments arranged by size:
299 192 169 42
SapI GCTCTTCn'nnn_
Cuts at: 0 515 702
Size: 515 187
SfcI C'TryA_G
Cuts at: 0 511 553 574 702
Size: 511 42 21 128
Fragments arranged by size:
511 128 42 21
SfoI GGC'GCC
Cuts at: 0 95 128 164 172 315 612 702
Size: 95 33 36 8 143 297 90
Fragments arranged by size:
297 143 95 90 36 33 8
SgrAI Cr'CCGG_yG
Cuts at: 0 290 702
Size: 290 412
SmaI CCC'GGG
Cuts at: 0 177 239 702
Size: 177 62 463
Fragments arranged by size:
463 177 62
SmlI C'TyrA_G
Cuts at: 0 646 702
Size: 646 56
SrfI GCCC'GGGC
Cuts at: 0 239 702
Size: 239 463
StuI AGG'CCT
Cuts at: 0 142 702
Size: 142 560
StyI C'CwwG_G
Cuts at: 0 26 702
Size: 26 676
XmaI C'CCGG_G
Cuts at: 0 175 237 702
Size: 175 62 465
Fragments arranged by size:
465 175 62
Enzymes that do cut:
AarI ApaI AscI AvaI BanI BanII BbeI BglI
Bme1580I BmrI BpmI BpuEI BsaHI BseRI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BssHII Bsu36I
BtgI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoO109I HaeII KasI MspA1I NaeI NarI NcoI NgoMIV
PasI PfoI PpuMI PspOMI PstI SacI SacII SapI
SfcI SfoI SgrAI SmaI SmlI SrfI StuI StyI
XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI
BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII EcoNI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI NdeI NheI NotI NruI NsiI
NspI PacI PciI PflMI PmeI PmlI PpiI PshAI
PsiI PspXI PsrI PvuI PvuII RsrII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SnaBI SpeI SphI
SspI SwaI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmnI ZraI