Restriction map for
pF1KB8773
(Linear) (Six-base) MAPSORT of: KIBB8773.seq Check: 9638 from: 1 to: 723
KIBB8773.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 428 723
Size: 428 295
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 723
Size: 37 686
AflIII A'CryG_T
Cuts at: 0 116 723
Size: 116 607
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 523 555 723
Size: 523 32 168
Fragments arranged by size:
523 168 32
AlwNI CAG_nnn'CTG
Cuts at: 0 379 434 723
Size: 379 55 289
Fragments arranged by size:
379 289 55
ApaI G_GGCC'C
Cuts at: 0 616 723
Size: 616 107
AvaI C'yCGr_G
Cuts at: 0 452 628 723
Size: 452 176 95
Fragments arranged by size:
452 176 95
BanI G'GyrC_C
Cuts at: 0 246 267 408 416 601 723
Size: 246 21 141 8 185 122
Fragments arranged by size:
246 185 141 122 21 8
BanII G_rGCy'C
Cuts at: 0 60 161 616 629 723
Size: 60 101 455 13 94
Fragments arranged by size:
455 101 94 60 13
BbeI G_GCGC'C
Cuts at: 0 271 723
Size: 271 452
BbsI GAAGACnn'nnnn_
Cuts at: 0 50 79 723
Size: 50 29 644
Fragments arranged by size:
644 50 29
BbvCI CC'TCA_GC
Cuts at: 0 399 723
Size: 399 324
BclI T'GATC_A
Cuts at: 0 486 723
Size: 486 237
Bme1580I G_kGCm'C
Cuts at: 0 333 616 723
Size: 333 283 107
Fragments arranged by size:
333 283 107
BmgBI CAC'GTC
Cuts at: 0 48 233 549 723
Size: 48 185 316 174
Fragments arranged by size:
316 185 174 48
BmrI ACTGGGnnnn_n'
Cuts at: 0 385 723
Size: 385 338
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 558 723
Size: 558 165
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 526 648 723
Size: 526 122 75
Fragments arranged by size:
526 122 75
Bpu10I CC'TnA_GC
Cuts at: 0 399 723
Size: 399 324
BsaI GGTCTCn'nnnn_
Cuts at: 0 291 712 723
Size: 291 421 11
Fragments arranged by size:
421 291 11
BsaAI yAC'GTr
Cuts at: 0 699 723
Size: 699 24
BsaHI Gr'CG_yC
Cuts at: 0 268 723
Size: 268 455
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 286 316 723
Size: 286 30 407
Fragments arranged by size:
407 286 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 226 723
Size: 226 497
BseYI C'CCAG_C
Cuts at: 0 520 723
Size: 520 203
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 723
Size: 329 394
BsiEI CG_ry'CG
Cuts at: 0 279 723
Size: 279 444
Bsp1286I G_dGCh'C
Cuts at: 0 60 161 333 616 629 723
Size: 60 101 172 283 13 94
Fragments arranged by size:
283 172 101 94 60 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 723
Size: 428 295
BsrBI CCG'CTC
Cuts at: 0 173 264 273 723
Size: 173 91 9 450
Fragments arranged by size:
450 173 91 9
BsrDI GCAATG_nn'
Cuts at: 0 182 723
Size: 182 541
BsrFI r'CCGG_y
Cuts at: 0 64 723
Size: 64 659
BssSI C'ACGA_G
Cuts at: 0 8 37 723
Size: 8 29 686
Fragments arranged by size:
686 29 8
BstAPI GCAn_nnn'nTGC
Cuts at: 0 337 723
Size: 337 386
BstXI CCAn_nnnn'nTGG
Cuts at: 0 528 723
Size: 528 195
BstYI r'GATC_y
Cuts at: 0 203 296 707 723
Size: 203 93 411 16
Fragments arranged by size:
411 203 93 16
BtgI C'CryG_G
Cuts at: 0 290 723
Size: 290 433
DraIII CAC_nnn'GTG
Cuts at: 0 702 723
Size: 702 21
DrdI GACnn_nn'nnGTC
Cuts at: 0 283 723
Size: 283 440
EaeI y'GGCC_r
Cuts at: 0 33 62 357 644 723
Size: 33 29 295 287 79
Fragments arranged by size:
295 287 79 33 29
EarI CTCTTCn'nnn_
Cuts at: 0 13 527 723
Size: 13 514 196
Fragments arranged by size:
514 196 13
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 526 648 723
Size: 37 489 122 75
Fragments arranged by size:
489 122 75 37
EcoO109I rG'GnC_Cy
Cuts at: 0 613 655 723
Size: 613 42 68
Fragments arranged by size:
613 68 42
HaeII r_GCGC'y
Cuts at: 0 271 434 723
Size: 271 163 289
Fragments arranged by size:
289 271 163
HindIII A'AGCT_T
Cuts at: 0 468 723
Size: 468 255
KasI G'GCGC_C
Cuts at: 0 267 723
Size: 267 456
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 701 723
Size: 701 22
MscI TGG'CCA
Cuts at: 0 35 723
Size: 35 688
MslI CAynn'nnrTG
Cuts at: 0 151 723
Size: 151 572
MspA1I CmG'CkG
Cuts at: 0 99 524 668 723
Size: 99 425 144 55
Fragments arranged by size:
425 144 99 55
NaeI GCC'GGC
Cuts at: 0 66 723
Size: 66 657
NarI GG'CG_CC
Cuts at: 0 268 723
Size: 268 455
NgoMIV G'CCGG_C
Cuts at: 0 64 723
Size: 64 659
NspI r_CATG'y
Cuts at: 0 120 150 723
Size: 120 30 573
Fragments arranged by size:
573 120 30
PciI A'CATG_T
Cuts at: 0 116 723
Size: 116 607
PflMI CCAn_nnn'nTGG
Cuts at: 0 702 723
Size: 702 21
PfoI T'CCnGG_A
Cuts at: 0 110 723
Size: 110 613
PmlI CAC'GTG
Cuts at: 0 699 723
Size: 699 24
PspOMI G'GGCC_C
Cuts at: 0 612 723
Size: 612 111
PspXI vC'TCGA_Gb
Cuts at: 0 628 723
Size: 628 95
PstI C_TGCA'G
Cuts at: 0 98 261 425 723
Size: 98 163 164 298
Fragments arranged by size:
298 164 163 98
PvuII CAG'CTG
Cuts at: 0 524 668 723
Size: 524 144 55
Fragments arranged by size:
524 144 55
RsrII CG'GwC_CG
Cuts at: 0 195 723
Size: 195 528
SapI GCTCTTCn'nnn_
Cuts at: 0 527 723
Size: 527 196
ScaI AGT'ACT
Cuts at: 0 581 723
Size: 581 142
SfcI C'TryA_G
Cuts at: 0 94 257 421 723
Size: 94 163 164 302
Fragments arranged by size:
302 164 163 94
SfoI GGC'GCC
Cuts at: 0 269 723
Size: 269 454
SmlI C'TyrA_G
Cuts at: 0 452 628 723
Size: 452 176 95
Fragments arranged by size:
452 176 95
StyI C'CwwG_G
Cuts at: 0 412 723
Size: 412 311
TatI w'GTAC_w
Cuts at: 0 579 723
Size: 579 144
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 217 249 286 318 354 386 723
Size: 217 32 37 32 36 32 337
Fragments arranged by size:
337 217 37 36 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 78 723
Size: 78 645
XhoI C'TCGA_G
Cuts at: 0 452 628 723
Size: 452 176 95
Fragments arranged by size:
452 176 95
Enzymes that do cut:
AarI AcuI AflIII AloI AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BclI Bme1580I BmgBI BmrI
BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaXI BseRI
BseYI BsgI BsiEI Bsp1286I BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstXI BstYI BtgI DraIII DrdI EaeI
EarI Eco57MI EcoO109I HaeII HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NgoMIV NspI PciI PflMI
PfoI PmlI PspOMI PspXI PstI PvuII RsrII SapI
ScaI SfcI SfoI SmlI StyI TatI TstI Tth111I
XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BfrBI BglI BglII BlpI
BmtI BpuEI BsaBI BsaWI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HincII HpaI KpnI MfeI MluI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PmeI PpiI PpuMI
PshAI PsiI PsrI PvuI SacI SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII XbaI XcmI
XmaI XmnI ZraI