Restriction map for
pF1KB8775
(Linear) (Six-base) MAPSORT of: KIBB8775.seq Check: 3595 from: 1 to: 741
KIBB8775.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 564 741
Size: 38 526 177
Fragments arranged by size:
526 177 38
AflIII A'CryG_T
Cuts at: 0 368 741
Size: 368 373
AleI CACnn'nnGTG
Cuts at: 0 442 487 741
Size: 442 45 254
Fragments arranged by size:
442 254 45
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 741
Size: 541 200
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 505 537 741
Size: 505 32 204
Fragments arranged by size:
505 204 32
ApoI r'AATT_y
Cuts at: 0 451 712 741
Size: 451 261 29
Fragments arranged by size:
451 261 29
BanI G'GyrC_C
Cuts at: 0 729 741
Size: 729 12
BanII G_rGCy'C
Cuts at: 0 421 436 741
Size: 421 15 305
Fragments arranged by size:
421 305 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 26 741
Size: 26 715
BmgBI CAC'GTC
Cuts at: 0 212 371 521 741
Size: 212 159 150 220
Fragments arranged by size:
220 212 159 150
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 455 741
Size: 455 286
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 130 741
Size: 73 57 611
Fragments arranged by size:
611 73 57
Bpu10I CC'TnA_GC
Cuts at: 0 430 741
Size: 430 311
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 358 388 568 598 741
Size: 358 30 180 30 143
Fragments arranged by size:
358 180 143 30 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 501 741
Size: 427 74 240
Fragments arranged by size:
427 240 74
BsiHKAI G_wGCw'C
Cuts at: 0 421 436 741
Size: 421 15 305
Fragments arranged by size:
421 305 15
BsmBI CGTCTCn'nnnn_
Cuts at: 0 202 741
Size: 202 539
Bsp1286I G_dGCh'C
Cuts at: 0 421 436 741
Size: 421 15 305
Fragments arranged by size:
421 305 15
BspHI T'CATG_A
Cuts at: 0 708 741
Size: 708 33
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 191 741
Size: 191 550
BstXI CCAn_nnnn'nTGG
Cuts at: 0 108 342 384 444 741
Size: 108 234 42 60 297
Fragments arranged by size:
297 234 108 60 42
Bsu36I CC'TnA_GG
Cuts at: 0 594 741
Size: 594 147
BtsI GCAGTG_nn'
Cuts at: 0 23 741
Size: 23 718
ClaI AT'CG_AT
Cuts at: 0 75 741
Size: 75 666
DraIII CAC_nnn'GTG
Cuts at: 0 178 741
Size: 178 563
EaeI y'GGCC_r
Cuts at: 0 2 741
Size: 2 739
EcoICRI GAG'CTC
Cuts at: 0 419 434 741
Size: 419 15 307
Fragments arranged by size:
419 307 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 38 73 130 564 741
Size: 38 35 57 434 177
Fragments arranged by size:
434 177 57 38 35
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 646 741
Size: 646 95
HincII GTy'rAC
Cuts at: 0 10 741
Size: 10 731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 401 741
Size: 401 340
MscI TGG'CCA
Cuts at: 0 4 741
Size: 4 737
MslI CAynn'nnrTG
Cuts at: 0 403 442 487 547 741
Size: 403 39 45 60 194
Fragments arranged by size:
403 194 60 45 39
PstI C_TGCA'G
Cuts at: 0 202 741
Size: 202 539
SacI G_AGCT'C
Cuts at: 0 421 436 741
Size: 421 15 305
Fragments arranged by size:
421 305 15
SfcI C'TryA_G
Cuts at: 0 198 741
Size: 198 543
StuI AGG'CCT
Cuts at: 0 40 462 741
Size: 40 422 279
Fragments arranged by size:
422 279 40
StyI C'CwwG_G
Cuts at: 0 630 741
Size: 630 111
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 358 390 741
Size: 358 32 351
Fragments arranged by size:
358 351 32
Tth111I GACn'n_nGTC
Cuts at: 0 478 741
Size: 478 263
XbaI T'CTAG_A
Cuts at: 0 717 741
Size: 717 24
Enzymes that do cut:
AcuI AflIII AleI AlfI AloI ApoI BanI BanII
BbsI BmgBI BplI BpmI Bpu10I BsaXI BsgI BsiHKAI
BsmBI Bsp1286I BspHI BspMI BstXI Bsu36I BtsI ClaI
DraIII EaeI EcoICRI Eco57MI FalI HincII MmeI MscI
MslI PstI SacI SfcI StuI StyI TstI Tth111I
XbaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmrI BmtI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI
BsiWI BsmI BspEI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI
CspCI DraI DrdI EagI EarI EciI EcoNI EcoO109I
EcoRI EcoRV FseI FspI FspAI HaeII Hin4I HindIII
HpaI KasI KpnI MfeI MluI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI SwaI TaqII TatI XcmI XhoI
XmaI XmnI ZraI