Restriction map for
pF1KB8776
(Linear) (Six-base) MAPSORT of: KIBB8776.seq Check: 3435 from: 1 to: 1623
KIBB8776.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1374 1623
Size: 1374 249
AgeI A'CCGG_T
Cuts at: 0 1012 1623
Size: 1012 611
AleI CACnn'nnGTG
Cuts at: 0 1013 1623
Size: 1013 610
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 236 1623
Size: 236 1387
AlwNI CAG_nnn'CTG
Cuts at: 0 1403 1623
Size: 1403 220
ApoI r'AATT_y
Cuts at: 0 24 534 836 894 1424 1623
Size: 24 510 302 58 530 199
Fragments arranged by size:
530 510 302 199 58 24
BanI G'GyrC_C
Cuts at: 0 1331 1623
Size: 1331 292
BanII G_rGCy'C
Cuts at: 0 562 1570 1623
Size: 562 1008 53
Fragments arranged by size:
1008 562 53
BlpI GC'TnA_GC
Cuts at: 0 813 1623
Size: 813 810
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 614 1118 1623
Size: 181 433 504 505
Fragments arranged by size:
505 504 433 181
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 159 1623
Size: 159 1464
BsaI GGTCTCn'nnnn_
Cuts at: 0 1432 1623
Size: 1432 191
BsaWI w'CCGG_w
Cuts at: 0 676 1012 1623
Size: 676 336 611
Fragments arranged by size:
676 611 336
BseYI C'CCAG_C
Cuts at: 0 322 1076 1528 1623
Size: 322 754 452 95
Fragments arranged by size:
754 452 322 95
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1139 1623
Size: 1139 484
BsiHKAI G_wGCw'C
Cuts at: 0 562 1570 1596 1623
Size: 562 1008 26 27
Fragments arranged by size:
1008 562 27 26
BsmI GAATG_Cn'
Cuts at: 0 784 868 1623
Size: 784 84 755
Fragments arranged by size:
784 755 84
BsmBI CGTCTCn'nnnn_
Cuts at: 0 248 1623
Size: 248 1375
Bsp1286I G_dGCh'C
Cuts at: 0 562 1570 1596 1623
Size: 562 1008 26 27
Fragments arranged by size:
1008 562 27 26
BsrFI r'CCGG_y
Cuts at: 0 1012 1532 1623
Size: 1012 520 91
Fragments arranged by size:
1012 520 91
BstYI r'GATC_y
Cuts at: 0 1172 1623
Size: 1172 451
BtgI C'CryG_G
Cuts at: 0 395 1613 1623
Size: 395 1218 10
Fragments arranged by size:
1218 395 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 548 1623
Size: 548 1075
EarI CTCTTCn'nnn_
Cuts at: 0 651 1623
Size: 651 972
EcoICRI GAG'CTC
Cuts at: 0 560 1568 1623
Size: 560 1008 55
Fragments arranged by size:
1008 560 55
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 181 614 1118 1374 1623
Size: 181 433 504 256 249
Fragments arranged by size:
504 433 256 249 181
EcoO109I rG'GnC_Cy
Cuts at: 0 1525 1623
Size: 1525 98
EcoRI G'AATT_C
Cuts at: 0 836 1424 1623
Size: 836 588 199
Fragments arranged by size:
836 588 199
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 729 761 1431 1463 1623
Size: 729 32 670 32 160
Fragments arranged by size:
729 670 160 32 32
HindIII A'AGCT_T
Cuts at: 0 797 1623
Size: 797 826
MslI CAynn'nnrTG
Cuts at: 0 945 1013 1349 1449 1623
Size: 945 68 336 100 174
Fragments arranged by size:
945 336 174 100 68
NcoI C'CATG_G
Cuts at: 0 395 1613 1623
Size: 395 1218 10
Fragments arranged by size:
1218 395 10
PflMI CCAn_nnn'nTGG
Cuts at: 0 396 1462 1623
Size: 396 1066 161
Fragments arranged by size:
1066 396 161
PfoI T'CCnGG_A
Cuts at: 0 1055 1623
Size: 1055 568
PpuMI rG'GwC_Cy
Cuts at: 0 1525 1623
Size: 1525 98
SacI G_AGCT'C
Cuts at: 0 562 1570 1623
Size: 562 1008 53
Fragments arranged by size:
1008 562 53
SanDI GG'GwC_CC
Cuts at: 0 1525 1623
Size: 1525 98
SgrAI Cr'CCGG_yG
Cuts at: 0 1012 1623
Size: 1012 611
SmlI C'TyrA_G
Cuts at: 0 138 1623
Size: 138 1485
StyI C'CwwG_G
Cuts at: 0 395 1613 1623
Size: 395 1218 10
Fragments arranged by size:
1218 395 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1090 1623
Size: 1090 533
TatI w'GTAC_w
Cuts at: 0 613 1497 1623
Size: 613 884 126
Fragments arranged by size:
884 613 126
XmnI GAAnn'nnTTC
Cuts at: 0 404 799 967 1135 1303 1471 1623
Size: 404 395 168 168 168 168 152
Fragments arranged by size:
404 395 168 168 168 168 152
Enzymes that do cut:
AcuI AgeI AleI AlfI AlwNI ApoI BanI BanII
BlpI BpmI BpuEI BsaI BsaWI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BsrFI BstYI BtgI BtgZI EarI
EcoICRI Eco57MI EcoO109I EcoRI Hin4I HindIII MslI NcoI
PflMI PfoI PpuMI SacI SanDI SgrAI SmlI StyI
TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BmtI
BplI Bpu10I BsaAI BsaBI BsaHI BsaXI BseRI BsiEI
BsiWI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DraIII DrdI EaeI EagI EciI
EcoNI EcoRV FalI FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MmeI MscI MspA1I
NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacII SalI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SmaI SnaBI SpeI SphI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI