Restriction map for
pF1KB8777
(Linear) (Six-base) MAPSORT of: KIBB8777.seq Check: 6502 from: 1 to: 771
KIBB8777.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 266 771
Size: 266 505
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 226 499 739 771
Size: 148 78 273 240 32
Fragments arranged by size:
273 240 148 78 32
AleI CACnn'nnGTG
Cuts at: 0 523 610 771
Size: 523 87 161
Fragments arranged by size:
523 161 87
AlwNI CAG_nnn'CTG
Cuts at: 0 133 258 564 584 771
Size: 133 125 306 20 187
Fragments arranged by size:
306 187 133 125 20
ApaI G_GGCC'C
Cuts at: 0 770 771
Size: 770 1
AvaI C'yCGr_G
Cuts at: 0 47 175 762 771
Size: 47 128 587 9
Fragments arranged by size:
587 128 47 9
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 409 442 771
Size: 409 33 329
Fragments arranged by size:
409 329 33
BamHI G'GATC_C
Cuts at: 0 380 771
Size: 380 391
BanI G'GyrC_C
Cuts at: 0 4 88 113 574 704 771
Size: 4 84 25 461 130 67
Fragments arranged by size:
461 130 84 67 25 4
BanII G_rGCy'C
Cuts at: 0 770 771
Size: 770 1
BbeI G_GCGC'C
Cuts at: 0 92 771
Size: 92 679
BbsI GAAGACnn'nnnn_
Cuts at: 0 135 771
Size: 135 636
BglI GCCn_nnn'nGGC
Cuts at: 0 301 771
Size: 301 470
BglII A'GATC_T
Cuts at: 0 224 771
Size: 224 547
Bme1580I G_kGCm'C
Cuts at: 0 7 118 770 771
Size: 7 111 652 1
Fragments arranged by size:
652 111 7 1
BmgBI CAC'GTC
Cuts at: 0 612 771
Size: 612 159
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 730 771
Size: 730 41
Bpu10I CC'TnA_GC
Cuts at: 0 268 771
Size: 268 503
BsaI GGTCTCn'nnnn_
Cuts at: 0 45 771
Size: 45 726
BsaHI Gr'CG_yC
Cuts at: 0 89 392 771
Size: 89 303 379
Fragments arranged by size:
379 303 89
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 211 241 454 484 771
Size: 211 30 213 30 287
Fragments arranged by size:
287 213 211 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 58 547 771
Size: 58 489 224
Fragments arranged by size:
489 224 58
BseYI C'CCAG_C
Cuts at: 0 730 771
Size: 730 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 244 424 547 771
Size: 64 180 180 123 224
Fragments arranged by size:
224 180 180 123 64
BsiHKAI G_wGCw'C
Cuts at: 0 203 771
Size: 203 568
Bsp1286I G_dGCh'C
Cuts at: 0 7 118 203 770 771
Size: 7 111 85 567 1
Fragments arranged by size:
567 111 85 7 1
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 771
Size: 266 505
BsrFI r'CCGG_y
Cuts at: 0 110 263 771
Size: 110 153 508
Fragments arranged by size:
508 153 110
BstEII G'GTnAC_C
Cuts at: 0 624 771
Size: 624 147
BstXI CCAn_nnnn'nTGG
Cuts at: 0 612 771
Size: 612 159
BstYI r'GATC_y
Cuts at: 0 224 279 380 771
Size: 224 55 101 391
Fragments arranged by size:
391 224 101 55
BtgI C'CryG_G
Cuts at: 0 647 771
Size: 647 124
BtsI GCAGTG_nn'
Cuts at: 0 441 771
Size: 441 330
EaeI y'GGCC_r
Cuts at: 0 188 332 614 771
Size: 188 144 282 157
Fragments arranged by size:
282 188 157 144
EarI CTCTTCn'nnn_
Cuts at: 0 67 76 771
Size: 67 9 695
Fragments arranged by size:
695 67 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 303 771
Size: 303 468
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 226 499 730 739 771
Size: 148 78 273 231 9 32
Fragments arranged by size:
273 231 148 78 32 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 166 460 771
Size: 166 294 311
Fragments arranged by size:
311 294 166
EcoO109I rG'GnC_Cy
Cuts at: 0 159 171 245 339 465 750 766 771
Size: 159 12 74 94 126 285 16 5
Fragments arranged by size:
285 159 126 94 74 16 12 5
HaeII r_GCGC'y
Cuts at: 0 92 413 737 771
Size: 92 321 324 34
Fragments arranged by size:
324 321 92 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 478 510 771
Size: 478 32 261
Fragments arranged by size:
478 261 32
KasI G'GCGC_C
Cuts at: 0 88 771
Size: 88 683
MscI TGG'CCA
Cuts at: 0 616 771
Size: 616 155
MslI CAynn'nnrTG
Cuts at: 0 523 610 771
Size: 523 87 161
Fragments arranged by size:
523 161 87
NarI GG'CG_CC
Cuts at: 0 89 771
Size: 89 682
NcoI C'CATG_G
Cuts at: 0 647 771
Size: 647 124
PasI CC'CwG_GG
Cuts at: 0 396 771
Size: 396 375
PfoI T'CCnGG_A
Cuts at: 0 236 771
Size: 236 535
PpuMI rG'GwC_Cy
Cuts at: 0 171 750 771
Size: 171 579 21
Fragments arranged by size:
579 171 21
PspOMI G'GGCC_C
Cuts at: 0 766 771
Size: 766 5
PstI C_TGCA'G
Cuts at: 0 151 461 589 771
Size: 151 310 128 182
Fragments arranged by size:
310 182 151 128
SbfI CC_TGCA'GG
Cuts at: 0 461 771
Size: 461 310
SfcI C'TryA_G
Cuts at: 0 147 457 585 771
Size: 147 310 128 186
Fragments arranged by size:
310 186 147 128
SfoI GGC'GCC
Cuts at: 0 90 771
Size: 90 681
SmaI CCC'GGG
Cuts at: 0 49 764 771
Size: 49 715 7
Fragments arranged by size:
715 49 7
StyI C'CwwG_G
Cuts at: 0 386 647 746 771
Size: 386 261 99 25
Fragments arranged by size:
386 261 99 25
TatI w'GTAC_w
Cuts at: 0 668 771
Size: 668 103
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 454 486 771
Size: 454 32 285
Fragments arranged by size:
454 285 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 393 771
Size: 393 378
XmaI C'CCGG_G
Cuts at: 0 47 762 771
Size: 47 715 9
Fragments arranged by size:
715 47 9
Enzymes that do cut:
AarI AcuI AleI AlwNI ApaI AvaI BaeI BamHI
BanI BanII BbeI BbsI BglI BglII Bme1580I BmgBI
BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspMI BsrFI BstEII BstXI BstYI BtgI
BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I HaeII
Hin4I KasI MscI MslI NarI NcoI PasI PfoI
PpuMI PspOMI PstI SbfI SfcI SfoI SmaI StyI
TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BbvCI BcgI BciVI BclI BfrBI BlpI BmrI
BmtI BplI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI KpnI MfeI
MluI MmeI MspA1I NaeI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI ScaI SexAI SfiI
SgfI SgrAI SmlI SnaBI SpeI SphI SrfI SspI
StuI SwaI TaqII Tth111I XbaI XhoI XmnI ZraI