Restriction map for
pF1KB8778
(Linear) (Six-base) MAPSORT of: KIBB8778.seq Check: 4127 from: 1 to: 774
KIBB8778.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 324 774
Size: 324 450
AfeI AGC'GCT
Cuts at: 0 292 774
Size: 292 482
AflIII A'CryG_T
Cuts at: 0 170 774
Size: 170 604
AlwNI CAG_nnn'CTG
Cuts at: 0 189 339 553 774
Size: 189 150 214 221
Fragments arranged by size:
221 214 189 150
ApaI G_GGCC'C
Cuts at: 0 128 510 674 774
Size: 128 382 164 100
Fragments arranged by size:
382 164 128 100
AscI GG'CGCG_CC
Cuts at: 0 256 774
Size: 256 518
AvaI C'yCGr_G
Cuts at: 0 121 141 160 503 527 774
Size: 121 20 19 343 24 247
Fragments arranged by size:
343 247 121 24 20 19
BanI G'GyrC_C
Cuts at: 0 424 774
Size: 424 350
BanII G_rGCy'C
Cuts at: 0 128 247 510 674 707 774
Size: 128 119 263 164 33 67
Fragments arranged by size:
263 164 128 119 67 33
BbeI G_GCGC'C
Cuts at: 0 428 774
Size: 428 346
BbsI GAAGACnn'nnnn_
Cuts at: 0 613 774
Size: 613 161
BbvCI CC'TCA_GC
Cuts at: 0 154 774
Size: 154 620
BglI GCCn_nnn'nGGC
Cuts at: 0 205 774
Size: 205 569
Bme1580I G_kGCm'C
Cuts at: 0 128 446 510 674 774
Size: 128 318 64 164 100
Fragments arranged by size:
318 164 128 100 64
BmtI G_CTAG'C
Cuts at: 0 101 774
Size: 101 673
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 774
Size: 373 401
Bpu10I CC'TnA_GC
Cuts at: 0 154 774
Size: 154 620
BsaI GGTCTCn'nnnn_
Cuts at: 0 706 774
Size: 706 68
BsaHI Gr'CG_yC
Cuts at: 0 425 774
Size: 425 349
BsaWI w'CCGG_w
Cuts at: 0 285 774
Size: 285 489
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 354 384 523 549 553 579 774
Size: 354 30 139 26 4 26 195
Fragments arranged by size:
354 195 139 30 26 26 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 129 664 774
Size: 55 74 535 110
Fragments arranged by size:
535 110 74 55
BseYI C'CCAG_C
Cuts at: 0 339 546 553 774
Size: 339 207 7 221
Fragments arranged by size:
339 221 207 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 195 774
Size: 195 579
BsiHKAI G_wGCw'C
Cuts at: 0 707 774
Size: 707 67
Bsp1286I G_dGCh'C
Cuts at: 0 128 247 446 510 674 707 774
Size: 128 119 199 64 164 33 67
Fragments arranged by size:
199 164 128 119 67 64 33
BspEI T'CCGG_A
Cuts at: 0 285 774
Size: 285 489
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 324 774
Size: 324 450
BsrBI CCG'CTC
Cuts at: 0 310 387 774
Size: 310 77 387
Fragments arranged by size:
387 310 77
BsrFI r'CCGG_y
Cuts at: 0 114 774
Size: 114 660
BssHII G'CGCG_C
Cuts at: 0 256 774
Size: 256 518
BstAPI GCAn_nnn'nTGC
Cuts at: 0 189 774
Size: 189 585
BtgI C'CryG_G
Cuts at: 0 276 420 659 774
Size: 276 144 239 115
Fragments arranged by size:
276 239 144 115
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 124 537 774
Size: 124 413 237
Fragments arranged by size:
413 237 124
EcoICRI GAG'CTC
Cuts at: 0 705 774
Size: 705 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 373 774
Size: 373 401
EcoNI CCTnn'n_nnAGG
Cuts at: 0 600 774
Size: 600 174
EcoO109I rG'GnC_Cy
Cuts at: 0 365 557 774
Size: 365 192 217
Fragments arranged by size:
365 217 192
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 651 774
Size: 651 123
FspI TGC'GCA
Cuts at: 0 297 352 774
Size: 297 55 422
Fragments arranged by size:
422 297 55
HaeII r_GCGC'y
Cuts at: 0 232 294 428 774
Size: 232 62 134 346
Fragments arranged by size:
346 232 134 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 354 386 523 549 555 581 774
Size: 354 32 137 26 6 26 193
Fragments arranged by size:
354 193 137 32 26 26 6
KasI G'GCGC_C
Cuts at: 0 424 774
Size: 424 350
MluI A'CGCG_T
Cuts at: 0 170 774
Size: 170 604
MspA1I CmG'CkG
Cuts at: 0 179 214 278 553 774
Size: 179 35 64 275 221
Fragments arranged by size:
275 221 179 64 35
NarI GG'CG_CC
Cuts at: 0 425 774
Size: 425 349
NcoI C'CATG_G
Cuts at: 0 659 774
Size: 659 115
NheI G'CTAG_C
Cuts at: 0 97 774
Size: 97 677
PflMI CCAn_nnn'nTGG
Cuts at: 0 553 774
Size: 553 221
PpuMI rG'GwC_Cy
Cuts at: 0 557 774
Size: 557 217
PspOMI G'GGCC_C
Cuts at: 0 124 506 670 774
Size: 124 382 164 104
Fragments arranged by size:
382 164 124 104
PvuII CAG'CTG
Cuts at: 0 179 774
Size: 179 595
SacI G_AGCT'C
Cuts at: 0 707 774
Size: 707 67
SacII CC_GC'GG
Cuts at: 0 279 774
Size: 279 495
SanDI GG'GwC_CC
Cuts at: 0 557 774
Size: 557 217
SfoI GGC'GCC
Cuts at: 0 426 774
Size: 426 348
SmaI CCC'GGG
Cuts at: 0 123 505 529 774
Size: 123 382 24 245
Fragments arranged by size:
382 245 123 24
StyI C'CwwG_G
Cuts at: 0 635 659 774
Size: 635 24 115
Fragments arranged by size:
635 115 24
XmaI C'CCGG_G
Cuts at: 0 121 503 527 774
Size: 121 382 24 247
Fragments arranged by size:
382 247 121 24
Enzymes that do cut:
AarI AfeI AflIII AlwNI ApaI AscI AvaI BanI
BanII BbeI BbsI BbvCI BglI Bme1580I BmtI BpmI
Bpu10I BsaI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BssHII BstAPI
BtgI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI
HaeII Hin4I KasI MluI MspA1I NarI NcoI NheI
PflMI PpuMI PspOMI PvuII SacI SacII SanDI SfoI
SmaI StyI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BcgI BciVI BclI BfrBI BglII BlpI
BmgBI BmrI BplI BpuEI BsaAI BsaBI BsiEI BsiWI
BsmI BsmBI BspHI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EaeI EagI EarI EcoRI EcoRV
FseI FspAI HincII HindIII HpaI KpnI MfeI MmeI
MscI MslI NaeI NdeI NgoMIV NotI NruI NsiI
NspI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PstI PvuI RsrII SalI
SapI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI