(Linear) (Six-base) MAPSORT of: KIBB8779.seq  Check: 4721  from: 1  to: 777



KIBB8779.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0      11     777 
   Size:         11     766
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     777 
   Size:        427     350
 
AleI CACnn'nnGTG

Cuts at:      0     646     777 
   Size:        646     131
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     407     777 
   Size:        407     370
 
AlwNI CAG_nnn'CTG

Cuts at:      0     351     777 
   Size:        351     426
 
ApaLI G'TGCA_C

Cuts at:      0     382     777 
   Size:        382     395
 
ApoI r'AATT_y

Cuts at:      0     245     777 
   Size:        245     532
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     298     332     777 
   Size:        298      34     445

  Fragments arranged by size: 

                 445     298      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     732     777 
   Size:        732      45
 
BglII A'GATC_T

Cuts at:      0     180     777 
   Size:        180     597
 
Bme1580I G_kGCm'C

Cuts at:      0     386     777 
   Size:        386     391
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     148     777 
   Size:        148     629
 
BsaHI Gr'CG_yC

Cuts at:      0     481     777 
   Size:        481     296
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      37     486     777 
   Size:         37     449     291

  Fragments arranged by size: 

                 449     291      37
 
BsiHKAI G_wGCw'C

Cuts at:      0     386     777 
   Size:        386     391
 
Bsp1286I G_dGCh'C

Cuts at:      0     386     777 
   Size:        386     391
 
BspHI T'CATG_A

Cuts at:      0     489     777 
   Size:        489     288
 
BsrDI GCAATG_nn'

Cuts at:      0     159     777 
   Size:        159     618
 
BstEII G'GTnAC_C

Cuts at:      0     621     777 
   Size:        621     156
 
BstYI r'GATC_y

Cuts at:      0     180     777 
   Size:        180     597
 
BtsI GCAGTG_nn'

Cuts at:      0     441     777 
   Size:        441     336
 
ClaI AT'CG_AT

Cuts at:      0     308     777 
   Size:        308     469
 
DraI TTT'AAA

Cuts at:      0     137     777 
   Size:        137     640
 
DraIII CAC_nnn'GTG

Cuts at:      0     507     777 
   Size:        507     270
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     427     777 
   Size:        427     350
 
EcoO109I rG'GnC_Cy

Cuts at:      0     255     777 
   Size:        255     522
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     539     571     666     698     777 
   Size:        539      32      95      32      79

  Fragments arranged by size: 

                 539      95      79      32      32
 
HincII GTy'rAC

Cuts at:      0      23     777 
   Size:         23     754
 
HpaI GTT'AAC

Cuts at:      0      23     777 
   Size:         23     754
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     386     509     777 
   Size:        386     123     268

  Fragments arranged by size: 

                 386     268     123
 
MslI CAynn'nnrTG

Cuts at:      0     389     646     777 
   Size:        389     257     131

  Fragments arranged by size: 

                 389     257     131
 
NdeI CA'TA_TG

Cuts at:      0     657     777 
   Size:        657     120
 
NspI r_CATG'y

Cuts at:      0     319     541     777 
   Size:        319     222     236

  Fragments arranged by size: 

                 319     236     222
 
PpuMI rG'GwC_Cy

Cuts at:      0     255     777 
   Size:        255     522
 
SfcI C'TryA_G

Cuts at:      0     147     457     777 
   Size:        147     310     320

  Fragments arranged by size: 

                 320     310     147
 
SmlI C'TyrA_G

Cuts at:      0     163     777 
   Size:        163     614
 
SphI G_CATG'C

Cuts at:      0     319     777 
   Size:        319     458
 
StyI C'CwwG_G

Cuts at:      0     104     777 
   Size:        104     673
 
TatI w'GTAC_w

Cuts at:      0     451     777 
   Size:        451     326
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     219     251     363     395     777 
   Size:        219      32     112      32     382

  Fragments arranged by size: 

                 382     219     112      32      32
 

 Enzymes that do cut:

     AccI     AcuI     AleI     AlfI    AlwNI    ApaLI     ApoI     BcgI
     BglI    BglII Bme1580I    BpuEI    BsaHI    BseRI  BsiHKAI Bsp1286I
    BspHI    BsrDI   BstEII    BstYI     BtsI     ClaI     DraI   DraIII
  Eco57MI EcoO109I    Hin4I   HincII     HpaI     MmeI     MslI     NdeI
     NspI    PpuMI     SfcI     SmlI     SphI     StyI     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AloI     ApaI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI
     BclI    BfrBI     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseYI     BsgI
    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI  BstZ17I   Bsu36I
     BtgI    BtgZI    CspCI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII  HindIII     KasI     KpnI     MfeI     MluI     MscI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI