Restriction map for
pF1KB8780
(Linear) (Six-base) MAPSORT of: KIBB8780.seq Check: 9606 from: 1 to: 777
KIBB8780.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 127 777
Size: 82 45 650
Fragments arranged by size:
650 82 45
AlwNI CAG_nnn'CTG
Cuts at: 0 60 351 777
Size: 60 291 426
Fragments arranged by size:
426 291 60
ApaI G_GGCC'C
Cuts at: 0 208 757 777
Size: 208 549 20
Fragments arranged by size:
549 208 20
AvaI C'yCGr_G
Cuts at: 0 333 777
Size: 333 444
BanII G_rGCy'C
Cuts at: 0 208 757 777
Size: 208 549 20
Fragments arranged by size:
549 208 20
BbsI GAAGACnn'nnnn_
Cuts at: 0 765 777
Size: 765 12
BglI GCCn_nnn'nGGC
Cuts at: 0 583 777
Size: 583 194
BlpI GC'TnA_GC
Cuts at: 0 406 777
Size: 406 371
Bme1580I G_kGCm'C
Cuts at: 0 208 757 777
Size: 208 549 20
Fragments arranged by size:
549 208 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 447 621 777
Size: 447 174 156
Fragments arranged by size:
447 174 156
BmtI G_CTAG'C
Cuts at: 0 232 777
Size: 232 545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 109 184 289 391 543 667 777
Size: 109 75 105 102 152 124 110
Fragments arranged by size:
152 124 110 109 105 102 75
BsaI GGTCTCn'nnnn_
Cuts at: 0 383 524 777
Size: 383 141 253
Fragments arranged by size:
383 253 141
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 664 777
Size: 664 113
BseYI C'CCAG_C
Cuts at: 0 53 355 553 587 625 689 777
Size: 53 302 198 34 38 64 88
Fragments arranged by size:
302 198 88 64 53 38 34
BsiHKAI G_wGCw'C
Cuts at: 0 239 777
Size: 239 538
Bsp1286I G_dGCh'C
Cuts at: 0 208 239 757 777
Size: 208 31 518 20
Fragments arranged by size:
518 208 31 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 386 726 777
Size: 386 340 51
Fragments arranged by size:
386 340 51
BsrBI CCG'CTC
Cuts at: 0 417 777
Size: 417 360
BsrFI r'CCGG_y
Cuts at: 0 659 777
Size: 659 118
BstXI CCAn_nnnn'nTGG
Cuts at: 0 611 777
Size: 611 166
BtsI GCAGTG_nn'
Cuts at: 0 352 676 777
Size: 352 324 101
Fragments arranged by size:
352 324 101
EaeI y'GGCC_r
Cuts at: 0 278 326 777
Size: 278 48 451
Fragments arranged by size:
451 278 48
EarI CTCTTCn'nnn_
Cuts at: 0 768 777
Size: 768 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 50 745 777
Size: 50 695 32
Fragments arranged by size:
695 50 32
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 82 109 127 184 289 391 543 667
Size: 82 27 18 57 105 102 152 124
Cuts at: 667 777
Size: 110
Fragments arranged by size:
152 124 110 105 102 82 57 27
18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 724 777
Size: 649 75 53
Fragments arranged by size:
649 75 53
EcoO109I rG'GnC_Cy
Cuts at: 0 568 693 754 777
Size: 568 125 61 23
Fragments arranged by size:
568 125 61 23
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 29 61 777
Size: 29 32 716
Fragments arranged by size:
716 32 29
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 777
Size: 272 505
MscI TGG'CCA
Cuts at: 0 280 328 777
Size: 280 48 449
Fragments arranged by size:
449 280 48
MspA1I CmG'CkG
Cuts at: 0 13 57 210 342 522 777
Size: 13 44 153 132 180 255
Fragments arranged by size:
255 180 153 132 44 13
NaeI GCC'GGC
Cuts at: 0 661 777
Size: 661 116
NgoMIV G'CCGG_C
Cuts at: 0 659 777
Size: 659 118
NheI G'CTAG_C
Cuts at: 0 228 777
Size: 228 549
PasI CC'CwG_GG
Cuts at: 0 593 777
Size: 593 184
PfoI T'CCnGG_A
Cuts at: 0 449 644 668 777
Size: 449 195 24 109
Fragments arranged by size:
449 195 109 24
PpuMI rG'GwC_Cy
Cuts at: 0 693 777
Size: 693 84
PspOMI G'GGCC_C
Cuts at: 0 204 753 777
Size: 204 549 24
Fragments arranged by size:
549 204 24
PstI C_TGCA'G
Cuts at: 0 44 347 447 683 777
Size: 44 303 100 236 94
Fragments arranged by size:
303 236 100 94 44
PvuII CAG'CTG
Cuts at: 0 57 342 522 777
Size: 57 285 180 255
Fragments arranged by size:
285 255 180 57
SfcI C'TryA_G
Cuts at: 0 40 343 443 679 777
Size: 40 303 100 236 98
Fragments arranged by size:
303 236 100 98 40
SmaI CCC'GGG
Cuts at: 0 335 777
Size: 335 442
StuI AGG'CCT
Cuts at: 0 655 777
Size: 655 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 211 585 777
Size: 211 374 192
Fragments arranged by size:
374 211 192
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 717 777
Size: 717 60
XmaI C'CCGG_G
Cuts at: 0 333 777
Size: 333 444
Enzymes that do cut:
AcuI AlwNI ApaI AvaI BanII BbsI BglI BlpI
Bme1580I BmrI BmtI BpmI BsaI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BstXI BtsI EaeI EarI
EciI Eco57MI EcoNI EcoO109I Hin4I MmeI MscI MspA1I
NaeI NgoMIV NheI PasI PfoI PpuMI PspOMI PstI
PvuII SfcI SmaI StuI TaqII XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglII BmgBI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI
BtgZI ClaI CspCI DraI DraIII DrdI EagI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MslI NarI
NcoI NdeI NotI NruI NsiI NspI PacI PciI
PflMI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TatI TstI
Tth111I XbaI XhoI XmnI ZraI