(Linear) (Six-base) MAPSORT of: KIBB8781.seq  Check: 860  from: 1  to: 786



KIBB8781.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     384     786 
   Size:        384     402
 
AleI CACnn'nnGTG

Cuts at:      0     671     786 
   Size:        671     115
 
AlwNI CAG_nnn'CTG

Cuts at:      0     261     786 
   Size:        261     525
 
ApoI r'AATT_y

Cuts at:      0     182     266     350     518     686     786 
   Size:        182      84      84     168     168     100

  Fragments arranged by size: 

                 182     168     168     100      84      84
 
BanII G_rGCy'C

Cuts at:      0     328     786 
   Size:        328     458
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      95     786 
   Size:         95     691
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     380     464     632     716     786 
   Size:        296      84      84     168      84      70

  Fragments arranged by size: 

                 296     168      84      84      84      70
 
BsaWI w'CCGG_w

Cuts at:      0     190     786 
   Size:        190     596
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     122     786 
   Size:        122     664
 
BseYI C'CCAG_C

Cuts at:      0      86     786 
   Size:         86     700
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      43     147     786 
   Size:         43     104     639

  Fragments arranged by size: 

                 639     104      43
 
Bsp1286I G_dGCh'C

Cuts at:      0     328     786 
   Size:        328     458
 
BsrDI GCAATG_nn'

Cuts at:      0     303     786 
   Size:        303     483
 
BsrGI T'GTAC_A

Cuts at:      0     379     786 
   Size:        379     407
 
BtsI GCAGTG_nn'

Cuts at:      0     135     786 
   Size:        135     651
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     285     320     786 
   Size:        285      35     466

  Fragments arranged by size: 

                 466     285      35
 
EarI CTCTTCn'nnn_

Cuts at:      0     585     786 
   Size:        585     201
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     296     380     384     464     632     716     786 
   Size:        296      84       4      80     168      84      70

  Fragments arranged by size: 

                 296     168      84      84      80      70       4
 
EcoRI G'AATT_C

Cuts at:      0     182     266     350     518     686     786 
   Size:        182      84      84     168     168     100

  Fragments arranged by size: 

                 182     168     168     100      84      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     265     685     786 
   Size:        265     420     101

  Fragments arranged by size: 

                 420     265     101
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     483     515     786 
   Size:        483      32     271

  Fragments arranged by size: 

                 483     271      32
 
HincII GTy'rAC

Cuts at:      0      18     786 
   Size:         18     768
 
HpaI GTT'AAC

Cuts at:      0      18     786 
   Size:         18     768
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     699     786 
   Size:        699      87
 
MslI CAynn'nnrTG

Cuts at:      0     375     671     786 
   Size:        375     296     115

  Fragments arranged by size: 

                 375     296     115
 
NdeI CA'TA_TG

Cuts at:      0     412     786 
   Size:        412     374
 
NspI r_CATG'y

Cuts at:      0     500     548     786 
   Size:        500      48     238

  Fragments arranged by size: 

                 500     238      48
 
PsiI TTA'TAA

Cuts at:      0     206     626     786 
   Size:        206     420     160

  Fragments arranged by size: 

                 420     206     160
 
TatI w'GTAC_w

Cuts at:      0     379     786 
   Size:        379     407
 
XmnI GAAnn'nnTTC

Cuts at:      0     438     522     786 
   Size:        438      84     264

  Fragments arranged by size: 

                 438     264      84
 

 Enzymes that do cut:

     AcuI     AleI    AlwNI     ApoI    BanII     BplI     BpmI    BsaWI
    BseRI    BseYI     BsgI Bsp1286I    BsrDI    BsrGI     BtsI    CspCI
     EarI  Eco57MI    EcoRI     FalI    Hin4I   HincII     HpaI     MmeI
     MslI     NdeI     NspI     PsiI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaBI    BsaHI    BsaXI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI
    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI    HaeII  HindIII
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI