Restriction map for
pF1KB8782
(Linear) (Six-base) MAPSORT of: KIBB8782.seq Check: 7683 from: 1 to: 819
KIBB8782.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
Acc65I G'GTAC_C
Cuts at: 0 106 819
Size: 106 713
AfeI AGC'GCT
Cuts at: 0 715 819
Size: 715 104
AflIII A'CryG_T
Cuts at: 0 530 819
Size: 530 289
ApaLI G'TGCA_C
Cuts at: 0 217 394 819
Size: 217 177 425
Fragments arranged by size:
425 217 177
AvaI C'yCGr_G
Cuts at: 0 538 700 819
Size: 538 162 119
Fragments arranged by size:
538 162 119
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 90 123 819
Size: 90 33 696
Fragments arranged by size:
696 90 33
BanI G'GyrC_C
Cuts at: 0 106 361 665 819
Size: 106 255 304 154
Fragments arranged by size:
304 255 154 106
BanII G_rGCy'C
Cuts at: 0 433 819
Size: 433 386
BbeI G_GCGC'C
Cuts at: 0 365 669 819
Size: 365 304 150
Fragments arranged by size:
365 304 150
BbsI GAAGACnn'nnnn_
Cuts at: 0 216 819
Size: 216 603
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 328 362 579 613 615 649 819
Size: 328 34 217 34 2 34 170
Fragments arranged by size:
328 217 170 34 34 34 2
BlpI GC'TnA_GC
Cuts at: 0 427 519 819
Size: 427 92 300
Fragments arranged by size:
427 300 92
Bme1580I G_kGCm'C
Cuts at: 0 221 398 819
Size: 221 177 421
Fragments arranged by size:
421 221 177
BmgBI CAC'GTC
Cuts at: 0 222 819
Size: 222 597
Bpu10I CC'TnA_GC
Cuts at: 0 499 819
Size: 499 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 391 819
Size: 391 428
BsaHI Gr'CG_yC
Cuts at: 0 362 596 666 819
Size: 362 234 70 153
Fragments arranged by size:
362 234 153 70
BseYI C'CCAG_C
Cuts at: 0 39 819
Size: 39 780
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 361 819
Size: 322 39 458
Fragments arranged by size:
458 322 39
BsiEI CG_ry'CG
Cuts at: 0 278 474 819
Size: 278 196 345
Fragments arranged by size:
345 278 196
BsiHKAI G_wGCw'C
Cuts at: 0 221 398 505 548 605 686 819
Size: 221 177 107 43 57 81 133
Fragments arranged by size:
221 177 133 107 81 57 43
Bsp1286I G_dGCh'C
Cuts at: 0 221 398 433 505 548 605 686 819
Size: 221 177 35 72 43 57 81 133
Fragments arranged by size:
221 177 133 81 72 57 43 35
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 296 383 819
Size: 296 87 436
Fragments arranged by size:
436 296 87
BsrFI r'CCGG_y
Cuts at: 0 131 358 652 819
Size: 131 227 294 167
Fragments arranged by size:
294 227 167 131
BsrGI T'GTAC_A
Cuts at: 0 257 819
Size: 257 562
BssHII G'CGCG_C
Cuts at: 0 334 784 801 819
Size: 334 450 17 18
Fragments arranged by size:
450 334 18 17
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 300 819
Size: 216 84 519
Fragments arranged by size:
519 216 84
BstEII G'GTnAC_C
Cuts at: 0 615 819
Size: 615 204
BstXI CCAn_nnnn'nTGG
Cuts at: 0 123 484 819
Size: 123 361 335
Fragments arranged by size:
361 335 123
BtgI C'CryG_G
Cuts at: 0 110 483 770 819
Size: 110 373 287 49
Fragments arranged by size:
373 287 110 49
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 633 819
Size: 633 186
DraIII CAC_nnn'GTG
Cuts at: 0 216 393 623 819
Size: 216 177 230 196
Fragments arranged by size:
230 216 196 177
EaeI y'GGCC_r
Cuts at: 0 83 113 275 471 819
Size: 83 30 162 196 348
Fragments arranged by size:
348 196 162 83 30
EagI C'GGCC_G
Cuts at: 0 275 471 819
Size: 275 196 348
Fragments arranged by size:
348 275 196
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 747 819
Size: 747 72
EcoO109I rG'GnC_Cy
Cuts at: 0 723 764 819
Size: 723 41 55
Fragments arranged by size:
723 55 41
FspI TGC'GCA
Cuts at: 0 350 819
Size: 350 469
HaeII r_GCGC'y
Cuts at: 0 145 365 444 669 717 819
Size: 145 220 79 225 48 102
Fragments arranged by size:
225 220 145 102 79 48
HincII GTy'rAC
Cuts at: 0 159 819
Size: 159 660
KasI G'GCGC_C
Cuts at: 0 361 665 819
Size: 361 304 154
Fragments arranged by size:
361 304 154
KpnI G_GTAC'C
Cuts at: 0 110 819
Size: 110 709
MfeI C'AATT_G
Cuts at: 0 50 819
Size: 50 769
MluI A'CGCG_T
Cuts at: 0 530 819
Size: 530 289
MscI TGG'CCA
Cuts at: 0 85 115 819
Size: 85 30 704
Fragments arranged by size:
704 85 30
MslI CAynn'nnrTG
Cuts at: 0 121 238 265 819
Size: 121 117 27 554
Fragments arranged by size:
554 121 117 27
MspA1I CmG'CkG
Cuts at: 0 772 819
Size: 772 47
NaeI GCC'GGC
Cuts at: 0 360 654 819
Size: 360 294 165
Fragments arranged by size:
360 294 165
NarI GG'CG_CC
Cuts at: 0 362 666 819
Size: 362 304 153
Fragments arranged by size:
362 304 153
NcoI C'CATG_G
Cuts at: 0 483 819
Size: 483 336
NgoMIV G'CCGG_C
Cuts at: 0 358 652 819
Size: 358 294 167
Fragments arranged by size:
358 294 167
NotI GC'GGCC_GC
Cuts at: 0 275 819
Size: 275 544
NspI r_CATG'y
Cuts at: 0 630 819
Size: 630 189
PsiI TTA'TAA
Cuts at: 0 35 819
Size: 35 784
PspXI vC'TCGA_Gb
Cuts at: 0 700 819
Size: 700 119
PstI C_TGCA'G
Cuts at: 0 746 819
Size: 746 73
SacII CC_GC'GG
Cuts at: 0 773 819
Size: 773 46
SexAI A'CCwGG_T
Cuts at: 0 389 819
Size: 389 430
SfcI C'TryA_G
Cuts at: 0 742 819
Size: 742 77
SfoI GGC'GCC
Cuts at: 0 363 667 819
Size: 363 304 152
Fragments arranged by size:
363 304 152
SgrAI Cr'CCGG_yG
Cuts at: 0 358 819
Size: 358 461
SmlI C'TyrA_G
Cuts at: 0 406 700 819
Size: 406 294 119
Fragments arranged by size:
406 294 119
SphI G_CATG'C
Cuts at: 0 630 819
Size: 630 189
StuI AGG'CCT
Cuts at: 0 698 819
Size: 698 121
StyI C'CwwG_G
Cuts at: 0 71 483 819
Size: 71 412 336
Fragments arranged by size:
412 336 71
TatI w'GTAC_w
Cuts at: 0 98 257 819
Size: 98 159 562
Fragments arranged by size:
562 159 98
Tth111I GACn'n_nGTC
Cuts at: 0 154 819
Size: 154 665
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 78 123 819
Size: 78 45 696
Fragments arranged by size:
696 78 45
XhoI C'TCGA_G
Cuts at: 0 700 819
Size: 700 119
Enzymes that do cut:
Acc65I AfeI AflIII ApaLI AvaI BaeI BanI BanII
BbeI BbsI BcgI BlpI Bme1580I BmgBI Bpu10I BpuEI
BsaHI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI
BsrGI BssHII BstAPI BstEII BstXI BtgI BtgZI DraIII
EaeI EagI EciI EcoO109I FspI HaeII HincII KasI
KpnI MfeI MluI MscI MslI MspA1I NaeI NarI
NcoI NgoMIV NotI NspI PsiI PspXI PstI SacII
SexAI SfcI SfoI SgrAI SmlI SphI StuI StyI
TatI Tth111I XcmI XhoI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BamHI BbvCI BciVI BclI BfrBI BglI
BglII BmrI BmtI BplI BpmI BsaI BsaAI BsaBI
BsaWI BsaXI BseRI BsiWI BsmI BsmBI BspEI BspHI
BsrBI BsrDI BssSI BstBI BstYI BstZ17I Bsu36I BtsI
ClaI CspCI DraI DrdI EarI EcoICRI Eco57MI EcoNI
EcoRI EcoRV FalI FseI FspAI Hin4I HindIII HpaI
MmeI NdeI NheI NruI NsiI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI
PsrI PvuI PvuII RsrII SacI SalI SanDI SapI
SbfI ScaI SfiI SgfI SmaI SnaBI SpeI SrfI
SspI SwaI TaqII TstI XbaI XmaI XmnI ZraI