Restriction map for
pF1KB8786
(Linear) (Six-base) MAPSORT of: KIBB8786.seq Check: 6484 from: 1 to: 891
KIBB8786.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
Acc65I G'GTAC_C
Cuts at: 0 410 891
Size: 410 481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 891
Size: 339 552
AflIII A'CryG_T
Cuts at: 0 584 761 891
Size: 584 177 130
Fragments arranged by size:
584 177 130
AleI CACnn'nnGTG
Cuts at: 0 442 493 891
Size: 442 51 398
Fragments arranged by size:
442 398 51
AlwNI CAG_nnn'CTG
Cuts at: 0 887 891
Size: 887 4
ApaI G_GGCC'C
Cuts at: 0 699 891
Size: 699 192
ApaLI G'TGCA_C
Cuts at: 0 580 891
Size: 580 311
AvaI C'yCGr_G
Cuts at: 0 673 891
Size: 673 218
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 394 427 891
Size: 394 33 464
Fragments arranged by size:
464 394 33
BanI G'GyrC_C
Cuts at: 0 168 410 891
Size: 168 242 481
Fragments arranged by size:
481 242 168
BanII G_rGCy'C
Cuts at: 0 699 841 848 891
Size: 699 142 7 43
Fragments arranged by size:
699 142 43 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 552 672 891
Size: 552 120 219
Fragments arranged by size:
552 219 120
BclI T'GATC_A
Cuts at: 0 248 524 791 891
Size: 248 276 267 100
Fragments arranged by size:
276 267 248 100
BglII A'GATC_T
Cuts at: 0 230 617 891
Size: 230 387 274
Fragments arranged by size:
387 274 230
Bme1580I G_kGCm'C
Cuts at: 0 518 584 699 891
Size: 518 66 115 192
Fragments arranged by size:
518 192 115 66
BmgBI CAC'GTC
Cuts at: 0 536 891
Size: 536 355
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 891
Size: 148 743
Bpu10I CC'TnA_GC
Cuts at: 0 847 891
Size: 847 44
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 891
Size: 259 632
BsaAI yAC'GTr
Cuts at: 0 283 891
Size: 283 608
BsaWI w'CCGG_w
Cuts at: 0 833 891
Size: 833 58
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 891
Size: 97 794
BseYI C'CCAG_C
Cuts at: 0 213 692 891
Size: 213 479 199
Fragments arranged by size:
479 213 199
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 456 891
Size: 456 435
BsiHKAI G_wGCw'C
Cuts at: 0 584 848 891
Size: 584 264 43
Fragments arranged by size:
584 264 43
BsmI GAATG_Cn'
Cuts at: 0 856 891
Size: 856 35
Bsp1286I G_dGCh'C
Cuts at: 0 518 584 699 841 848 891
Size: 518 66 115 142 7 43
Fragments arranged by size:
518 142 115 66 43 7
BspEI T'CCGG_A
Cuts at: 0 833 891
Size: 833 58
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 872 891
Size: 872 19
BsrDI GCAATG_nn'
Cuts at: 0 807 891
Size: 807 84
BssSI C'ACGA_G
Cuts at: 0 65 891
Size: 65 826
BstYI r'GATC_y
Cuts at: 0 230 617 891
Size: 230 387 274
Fragments arranged by size:
387 274 230
BtgI C'CryG_G
Cuts at: 0 491 891
Size: 491 400
BtsI GCAGTG_nn'
Cuts at: 0 403 891
Size: 403 488
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 264 299 891
Size: 264 35 592
Fragments arranged by size:
592 264 35
EaeI y'GGCC_r
Cuts at: 0 197 891
Size: 197 694
EarI CTCTTCn'nnn_
Cuts at: 0 775 891
Size: 775 116
EcoICRI GAG'CTC
Cuts at: 0 846 891
Size: 846 45
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 148 339 891
Size: 148 191 552
Fragments arranged by size:
552 191 148
EcoO109I rG'GnC_Cy
Cuts at: 0 696 891
Size: 696 195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 888 891
Size: 888 3
KpnI G_GTAC'C
Cuts at: 0 414 891
Size: 414 477
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 90 891
Size: 80 10 801
Fragments arranged by size:
801 80 10
MscI TGG'CCA
Cuts at: 0 199 891
Size: 199 692
MslI CAynn'nnrTG
Cuts at: 0 442 490 493 891
Size: 442 48 3 398
Fragments arranged by size:
442 398 48 3
MspA1I CmG'CkG
Cuts at: 0 549 891
Size: 549 342
NcoI C'CATG_G
Cuts at: 0 491 891
Size: 491 400
NspI r_CATG'y
Cuts at: 0 588 765 789 891
Size: 588 177 24 102
Fragments arranged by size:
588 177 102 24
PciI A'CATG_T
Cuts at: 0 584 761 891
Size: 584 177 130
Fragments arranged by size:
584 177 130
PmlI CAC'GTG
Cuts at: 0 283 891
Size: 283 608
PspOMI G'GGCC_C
Cuts at: 0 695 891
Size: 695 196
PstI C_TGCA'G
Cuts at: 0 880 891
Size: 880 11
RsrII CG'GwC_CG
Cuts at: 0 378 891
Size: 378 513
SacI G_AGCT'C
Cuts at: 0 848 891
Size: 848 43
SfcI C'TryA_G
Cuts at: 0 189 876 891
Size: 189 687 15
Fragments arranged by size:
687 189 15
SmlI C'TyrA_G
Cuts at: 0 274 891
Size: 274 617
StyI C'CwwG_G
Cuts at: 0 491 891
Size: 491 400
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 186 891
Size: 186 705
TatI w'GTAC_w
Cuts at: 0 176 891
Size: 176 715
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 650 682 891
Size: 650 32 209
Fragments arranged by size:
650 209 32
XmnI GAAnn'nnTTC
Cuts at: 0 51 891
Size: 51 840
Enzymes that do cut:
Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI AvaI
BaeI BanI BanII BbsI BclI BglII Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaAI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspEI BspMI BsrDI BssSI BstYI
BtgI BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I
FalI KpnI MmeI MscI MslI MspA1I NcoI NspI
PciI PmlI PspOMI PstI RsrII SacI SfcI SmlI
StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AgeI AhdI
AlfI AloI ApoI AscI AseI AsiSI AvrII BamHI
BbeI BbvCI BcgI BciVI BfrBI BglI BlpI BmrI
BmtI BplI BsaI BsaBI BsaHI BsaXI BsiEI BsiWI
BsmBI BspHI BsrBI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI Tth111I XbaI XcmI XhoI XmaI ZraI