(Linear) (Six-base) MAPSORT of: KIBB8792.seq  Check: 1682  from: 1  to: 981



KIBB8792.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AleI CACnn'nnGTG

Cuts at:      0     167     251     502     754     838     981 
   Size:        167      84     251     252      84     143

  Fragments arranged by size: 

                 252     251     167     143      84      84
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      26     981 
   Size:         26     955
 
AlwNI CAG_nnn'CTG

Cuts at:      0      40     981 
   Size:         40     941
 
ApaLI G'TGCA_C

Cuts at:      0     302     637     981 
   Size:        302     335     344

  Fragments arranged by size: 

                 344     335     302
 
ApoI r'AATT_y

Cuts at:      0     158     326     409     493     661     745     829     981 
   Size:        158     168      83      84     168      84      84     152

  Fragments arranged by size: 

                 168     168     158     152      84      84      84      83
            
 
Bme1580I G_kGCm'C

Cuts at:      0     138     306     641     981 
   Size:        138     168     335     340

  Fragments arranged by size: 

                 340     335     168     138
 
BsiHKAI G_wGCw'C

Cuts at:      0     306     641     981 
   Size:        306     335     340

  Fragments arranged by size: 

                 340     335     306
 
BsmI GAATG_Cn'

Cuts at:      0     190     540     981 
   Size:        190     350     441

  Fragments arranged by size: 

                 441     350     190
 
Bsp1286I G_dGCh'C

Cuts at:      0     138     306     641     981 
   Size:        138     168     335     340

  Fragments arranged by size: 

                 340     335     168     138
 
BsrDI GCAATG_nn'

Cuts at:      0      20     367     981 
   Size:         20     347     614

  Fragments arranged by size: 

                 614     347      20
 
BsrGI T'GTAC_A

Cuts at:      0     438     981 
   Size:        438     543
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      40     981 
   Size:         40     941
 
BtsI GCAGTG_nn'

Cuts at:      0     870     981 
   Size:        870     111
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     177     212     981 
   Size:        177      35     769

  Fragments arranged by size: 

                 769     177      35
 
DraI TTT'AAA

Cuts at:      0      23     981 
   Size:         23     958
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     312     981 
   Size:        312     669
 
EcoRI G'AATT_C

Cuts at:      0     158     326     493     661     745     829     981 
   Size:        158     168     167     168      84      84     152

  Fragments arranged by size: 

                 168     168     167     158     152      84      84
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     977     981 
   Size:        977       4
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     291     323     981 
   Size:        291      32     658

  Fragments arranged by size: 

                 658     291      32
 
MfeI C'AATT_G

Cuts at:      0     800     981 
   Size:        800     181
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     346     915     981 
   Size:        346     569      66

  Fragments arranged by size: 

                 569     346      66
 
MslI CAynn'nnrTG

Cuts at:      0     167     183     251     335     418     502     586     670 
   Size:        167      16      68      84      83      84      84      84

Cuts at:    670     686     754     838     922     981 
   Size:         16      68      84      84      59

  Fragments arranged by size: 

                 167      84      84      84      84      84      84      83
                  68      68      59      16      16
 
NdeI CA'TA_TG

Cuts at:      0     180     683     981 
   Size:        180     503     298

  Fragments arranged by size: 

                 503     298     180
 
PsiI TTA'TAA

Cuts at:      0     350     981 
   Size:        350     631
 
SpeI A'CTAG_T

Cuts at:      0     250     981 
   Size:        250     731
 
StuI AGG'CCT

Cuts at:      0     373     960     981 
   Size:        373     587      21

  Fragments arranged by size: 

                 587     373      21
 
TatI w'GTAC_w

Cuts at:      0     438     981 
   Size:        438     543
 
XmnI GAAnn'nnTTC

Cuts at:      0     708     960     981 
   Size:        708     252      21

  Fragments arranged by size: 

                 708     252      21
 

 Enzymes that do cut:

     AleI     AlfI    AlwNI    ApaLI     ApoI Bme1580I  BsiHKAI     BsmI
 Bsp1286I    BsrDI    BsrGI   BstAPI     BtsI    CspCI     DraI     EciI
    EcoRI     FalI    Hin4I     MfeI     MmeI     MslI     NdeI     PsiI
     SpeI     StuI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AloI     ApaI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrFI
   BssHII    BssSI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI   DraIII     DrdI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRV     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MluI     MscI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SphI     SrfI     SspI     StyI     SwaI
    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI