Restriction map for
pF1KB8793
(Linear) (Six-base) MAPSORT of: KIBB8793.seq Check: 939 from: 1 to: 990
KIBB8793.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AccI GT'mk_AC
Cuts at: 0 197 990
Size: 197 793
AfeI AGC'GCT
Cuts at: 0 379 990
Size: 379 611
AleI CACnn'nnGTG
Cuts at: 0 319 990
Size: 319 671
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 935 990
Size: 935 55
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 481 513 990
Size: 481 32 477
Fragments arranged by size:
481 477 32
ApaI G_GGCC'C
Cuts at: 0 465 990
Size: 465 525
BamHI G'GATC_C
Cuts at: 0 3 623 990
Size: 3 620 367
Fragments arranged by size:
620 367 3
BanI G'GyrC_C
Cuts at: 0 958 990
Size: 958 32
BanII G_rGCy'C
Cuts at: 0 465 850 909 990
Size: 465 385 59 81
Fragments arranged by size:
465 385 81 59
BbsI GAAGACnn'nnnn_
Cuts at: 0 93 990
Size: 93 897
BbvCI CC'TCA_GC
Cuts at: 0 205 923 990
Size: 205 718 67
Fragments arranged by size:
718 205 67
BlpI GC'TnA_GC
Cuts at: 0 358 478 990
Size: 358 120 512
Fragments arranged by size:
512 358 120
Bme1580I G_kGCm'C
Cuts at: 0 465 961 990
Size: 465 496 29
Fragments arranged by size:
496 465 29
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 883 990
Size: 621 262 107
Fragments arranged by size:
621 262 107
Bpu10I CC'TnA_GC
Cuts at: 0 205 349 923 990
Size: 205 144 574 67
Fragments arranged by size:
574 205 144 67
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 871 990
Size: 871 119
BsaI GGTCTCn'nnnn_
Cuts at: 0 79 990
Size: 79 911
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 688 990
Size: 688 302
BsiEI CG_ry'CG
Cuts at: 0 443 990
Size: 443 547
BsiHKAI G_wGCw'C
Cuts at: 0 155 364 990
Size: 155 209 626
Fragments arranged by size:
626 209 155
BsmI GAATG_Cn'
Cuts at: 0 719 990
Size: 719 271
Bsp1286I G_dGCh'C
Cuts at: 0 155 364 465 850 909 961 990
Size: 155 209 101 385 59 52 29
Fragments arranged by size:
385 209 155 101 59 52 29
BsrDI GCAATG_nn'
Cuts at: 0 116 917 990
Size: 116 801 73
Fragments arranged by size:
801 116 73
BstYI r'GATC_y
Cuts at: 0 3 623 743 990
Size: 3 620 120 247
Fragments arranged by size:
620 247 120 3
BtsI GCAGTG_nn'
Cuts at: 0 557 822 868 990
Size: 557 265 46 122
Fragments arranged by size:
557 265 122 46
EaeI y'GGCC_r
Cuts at: 0 212 990
Size: 212 778
EarI CTCTTCn'nnn_
Cuts at: 0 235 990
Size: 235 755
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 621 883 990
Size: 621 262 107
Fragments arranged by size:
621 262 107
EcoO109I rG'GnC_Cy
Cuts at: 0 462 990
Size: 462 528
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 82 990
Size: 82 908
FspI TGC'GCA
Cuts at: 0 178 990
Size: 178 812
HaeII r_GCGC'y
Cuts at: 0 381 990
Size: 381 609
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 511 543 966 990
Size: 511 32 423 24
Fragments arranged by size:
511 423 32 24
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 990
Size: 31 959
MslI CAynn'nnrTG
Cuts at: 0 319 322 990
Size: 319 3 668
Fragments arranged by size:
668 319 3
MspA1I CmG'CkG
Cuts at: 0 439 990
Size: 439 551
NspI r_CATG'y
Cuts at: 0 177 357 990
Size: 177 180 633
Fragments arranged by size:
633 180 177
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 175 207 990
Size: 175 32 783
Fragments arranged by size:
783 175 32
PshAI GACnn'nnGTC
Cuts at: 0 432 990
Size: 432 558
PsiI TTA'TAA
Cuts at: 0 250 990
Size: 250 740
PspOMI G'GGCC_C
Cuts at: 0 461 990
Size: 461 529
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 18 50 990
Size: 18 32 940
Fragments arranged by size:
940 32 18
PstI C_TGCA'G
Cuts at: 0 564 875 914 990
Size: 564 311 39 76
Fragments arranged by size:
564 311 76 39
SfcI C'TryA_G
Cuts at: 0 560 871 910 990
Size: 560 311 39 80
Fragments arranged by size:
560 311 80 39
SmlI C'TyrA_G
Cuts at: 0 886 990
Size: 886 104
SphI G_CATG'C
Cuts at: 0 177 357 990
Size: 177 180 633
Fragments arranged by size:
633 180 177
SspI AAT'ATT
Cuts at: 0 737 990
Size: 737 253
StuI AGG'CCT
Cuts at: 0 347 990
Size: 347 643
TatI w'GTAC_w
Cuts at: 0 34 990
Size: 34 956
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 486 518 615 647 990
Size: 486 32 97 32 343
Fragments arranged by size:
486 343 97 32 32
XmnI GAAnn'nnTTC
Cuts at: 0 187 990
Size: 187 803
Enzymes that do cut:
AccI AfeI AleI AlfI AloI ApaI BamHI BanI
BanII BbsI BbvCI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaI BseRI BsiEI BsiHKAI BsmI Bsp1286I BsrDI BstYI
BtsI EaeI EarI Eco57MI EcoO109I FalI FspI HaeII
Hin4I MmeI MslI MspA1I NspI PpiI PshAI PsiI
PspOMI PsrI PstI SfcI SmlI SphI SspI StuI
TatI TstI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AflII AflIII AgeI
AhdI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BmtI BplI BsaAI BsaBI BsaHI
BsaWI BsaXI BseYI BsgI BsiWI BsmBI BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PfoI PmeI PmlI PpuMI PspXI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI
StyI SwaI TaqII Tth111I XbaI XcmI XhoI XmaI
ZraI