Restriction map for
pF1KB8794
(Linear) (Six-base) MAPSORT of: KIBB8794.seq Check: 7818 from: 1 to: 990
KIBB8794.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 704 990
Size: 704 286
AccI GT'mk_AC
Cuts at: 0 378 395 794 990
Size: 378 17 399 196
Fragments arranged by size:
399 378 196 17
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 990
Size: 670 320
AflIII A'CryG_T
Cuts at: 0 547 990
Size: 547 443
AleI CACnn'nnGTG
Cuts at: 0 157 990
Size: 157 833
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 308 643 990
Size: 308 335 347
Fragments arranged by size:
347 335 308
ApaI G_GGCC'C
Cuts at: 0 76 89 990
Size: 76 13 901
Fragments arranged by size:
901 76 13
BanI G'GyrC_C
Cuts at: 0 151 487 692 990
Size: 151 336 205 298
Fragments arranged by size:
336 298 205 151
BanII G_rGCy'C
Cuts at: 0 76 89 990
Size: 76 13 901
Fragments arranged by size:
901 76 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 291 990
Size: 291 699
BclI T'GATC_A
Cuts at: 0 872 990
Size: 872 118
BglI GCCn_nnn'nGGC
Cuts at: 0 536 745 990
Size: 536 209 245
Fragments arranged by size:
536 245 209
BlpI GC'TnA_GC
Cuts at: 0 312 990
Size: 312 678
Bme1580I G_kGCm'C
Cuts at: 0 76 89 202 219 492 990
Size: 76 13 113 17 273 498
Fragments arranged by size:
498 273 113 76 17 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 974 990
Size: 974 16
Bpu10I CC'TnA_GC
Cuts at: 0 45 990
Size: 45 945
BseYI C'CCAG_C
Cuts at: 0 75 671 804 990
Size: 75 596 133 186
Fragments arranged by size:
596 186 133 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 32 268 931 990
Size: 32 236 663 59
Fragments arranged by size:
663 236 59 32
BsiEI CG_ry'CG
Cuts at: 0 399 990
Size: 399 591
BsiHKAI G_wGCw'C
Cuts at: 0 140 990
Size: 140 850
BsmI GAATG_Cn'
Cuts at: 0 177 990
Size: 177 813
Bsp1286I G_dGCh'C
Cuts at: 0 76 89 140 202 219 492 990
Size: 76 13 51 62 17 273 498
Fragments arranged by size:
498 273 76 62 51 17 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 266 704 990
Size: 266 438 286
Fragments arranged by size:
438 286 266
BsrBI CCG'CTC
Cuts at: 0 974 990
Size: 974 16
BsrDI GCAATG_nn'
Cuts at: 0 356 596 734 990
Size: 356 240 138 256
Fragments arranged by size:
356 256 240 138
BsrGI T'GTAC_A
Cuts at: 0 413 990
Size: 413 577
BstAPI GCAn_nnn'nTGC
Cuts at: 0 659 699 918 990
Size: 659 40 219 72
Fragments arranged by size:
659 219 72 40
BstXI CCAn_nnnn'nTGG
Cuts at: 0 195 990
Size: 195 795
BstYI r'GATC_y
Cuts at: 0 956 990
Size: 956 34
Bsu36I CC'TnA_GG
Cuts at: 0 119 990
Size: 119 871
BtgI C'CryG_G
Cuts at: 0 248 749 990
Size: 248 501 241
Fragments arranged by size:
501 248 241
BtsI GCAGTG_nn'
Cuts at: 0 106 990
Size: 106 884
DraIII CAC_nnn'GTG
Cuts at: 0 717 990
Size: 717 273
EaeI y'GGCC_r
Cuts at: 0 185 245 383 593 719 746 990
Size: 185 60 138 210 126 27 244
Fragments arranged by size:
244 210 185 138 126 60 27
EarI CTCTTCn'nnn_
Cuts at: 0 954 990
Size: 954 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 670 990
Size: 670 320
EcoO109I rG'GnC_Cy
Cuts at: 0 85 990
Size: 85 905
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 978 990
Size: 978 12
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 802 834 990
Size: 802 32 156
Fragments arranged by size:
802 156 32
HincII GTy'rAC
Cuts at: 0 396 990
Size: 396 594
MscI TGG'CCA
Cuts at: 0 187 385 595 721 990
Size: 187 198 210 126 269
Fragments arranged by size:
269 210 198 187 126
MslI CAynn'nnrTG
Cuts at: 0 157 193 990
Size: 157 36 797
Fragments arranged by size:
797 157 36
MspA1I CmG'CkG
Cuts at: 0 40 316 690 990
Size: 40 276 374 300
Fragments arranged by size:
374 300 276 40
NspI r_CATG'y
Cuts at: 0 551 990
Size: 551 439
PasI CC'CwG_GG
Cuts at: 0 492 990
Size: 492 498
PciI A'CATG_T
Cuts at: 0 547 990
Size: 547 443
PflMI CCAn_nnn'nTGG
Cuts at: 0 717 990
Size: 717 273
PspOMI G'GGCC_C
Cuts at: 0 72 85 990
Size: 72 13 905
Fragments arranged by size:
905 72 13
PstI C_TGCA'G
Cuts at: 0 632 990
Size: 632 358
PvuII CAG'CTG
Cuts at: 0 40 990
Size: 40 950
SalI G'TCGA_C
Cuts at: 0 394 990
Size: 394 596
SfcI C'TryA_G
Cuts at: 0 628 990
Size: 628 362
StuI AGG'CCT
Cuts at: 0 124 269 990
Size: 124 145 721
Fragments arranged by size:
721 145 124
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 796 990
Size: 796 194
TatI w'GTAC_w
Cuts at: 0 413 990
Size: 413 577
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 228 552 579 747 990
Size: 228 324 27 168 243
Fragments arranged by size:
324 243 228 168 27
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI ApaI BanI
BanII BbsI BclI BglI BlpI Bme1580I BmrI Bpu10I
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI
BsrDI BsrGI BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EarI Eco57MI EcoO109I FalI Hin4I HincII
MscI MslI MspA1I NspI PasI PciI PflMI PspOMI
PstI PvuII SalI SfcI StuI TaqII TatI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AlwNI ApaLI ApoI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI BglII
BmgBI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BsiWI BsmBI BspEI BspHI
BsrFI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DrdI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI