(Linear) (Six-base) MAPSORT of: KIBB8795.seq  Check: 1881  from: 1  to: 993



KIBB8795.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:26  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     831     993 
   Size:        328     503     162

  Fragments arranged by size: 

                 503     328     162
 
AflII C'TTAA_G

Cuts at:      0     684     993 
   Size:        684     309
 
AflIII A'CryG_T

Cuts at:      0     592     993 
   Size:        592     401
 
ApoI r'AATT_y

Cuts at:      0      18     629     993 
   Size:         18     611     364

  Fragments arranged by size: 

                 611     364      18
 
BanI G'GyrC_C

Cuts at:      0     368     993 
   Size:        368     625
 
BclI T'GATC_A

Cuts at:      0     786     993 
   Size:        786     207
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     659     827     993 
   Size:        659     168     166

  Fragments arranged by size: 

                 659     168     166
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     845     897     993 
   Size:        845      52      96

  Fragments arranged by size: 

                 845      96      52
 
BsaWI w'CCGG_w

Cuts at:      0     469     993 
   Size:        469     524
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      18     991     993 
   Size:         18     973       2

  Fragments arranged by size: 

                 973      18       2
 
BsmI GAATG_Cn'

Cuts at:      0     551     993 
   Size:        551     442
 
BtsI GCAGTG_nn'

Cuts at:      0     330     993 
   Size:        330     663
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     328     659     827     831     993 
   Size:        328     331     168       4     162

  Fragments arranged by size: 

                 331     328     168     162       4
 
EcoRI G'AATT_C

Cuts at:      0     629     993 
   Size:        629     364
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      61      93     702     734     993 
   Size:         61      32     609      32     259

  Fragments arranged by size: 

                 609     259      61      32      32
 
HindIII A'AGCT_T

Cuts at:      0     254     993 
   Size:        254     739
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     412     993 
   Size:        412     581
 
NspI r_CATG'y

Cuts at:      0     323     596     993 
   Size:        323     273     397

  Fragments arranged by size: 

                 397     323     273
 
PciI A'CATG_T

Cuts at:      0     592     993 
   Size:        592     401
 
PshAI GACnn'nnGTC

Cuts at:      0      92     993 
   Size:         92     901
 
ScaI AGT'ACT

Cuts at:      0      53     993 
   Size:         53     940
 
SmlI C'TyrA_G

Cuts at:      0     684     860     876     993 
   Size:        684     176      16     117

  Fragments arranged by size: 

                 684     176     117      16
 
TatI w'GTAC_w

Cuts at:      0      51     154     198     993 
   Size:         51     103      44     795

  Fragments arranged by size: 

                 795     103      51      44
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     579     611     993 
   Size:        579      32     382

  Fragments arranged by size: 

                 579     382      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     340     844     993 
   Size:        340     504     149

  Fragments arranged by size: 

                 504     340     149
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     ApoI     BanI     BclI     BpmI    BpuEI
    BsaWI    BseRI     BsmI     BtsI  Eco57MI    EcoRI    Hin4I  HindIII
     MmeI     NspI     PciI    PshAI     ScaI     SmlI     TatI     TstI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI
    BsaHI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII     HpaI     KasI
     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII  Tth111I     XbaI     XcmI
     XhoI     XmaI     ZraI