(Linear) (Six-base) MAPSORT of: KIBB8798.seq  Check: 8349  from: 1  to: 1008



KIBB8798.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AccI GT'mk_AC

Cuts at:      0     515    1008 
   Size:        515     493
 
AflIII A'CryG_T

Cuts at:      0      16    1008 
   Size:         16     992
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     217    1008 
   Size:        217     791
 
ApoI r'AATT_y

Cuts at:      0     433    1008 
   Size:        433     575
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     958    1008 
   Size:        958      50
 
BfrBI ATG'CAT

Cuts at:      0     297     467    1008 
   Size:        297     170     541

  Fragments arranged by size: 

                 541     297     170
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     499    1008 
   Size:        499     509
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     613    1008 
   Size:        613     395
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     213    1008 
   Size:        213     795
 
BspHI T'CATG_A

Cuts at:      0     209    1008 
   Size:        209     799
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     595    1008 
   Size:        595     413
 
BstZ17I GTA'TAC

Cuts at:      0     516    1008 
   Size:        516     492
 
BtgI C'CryG_G

Cuts at:      0      44    1008 
   Size:         44     964
 
BtsI GCAGTG_nn'

Cuts at:      0     201    1008 
   Size:        201     807
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     609     644    1008 
   Size:        609      35     364

  Fragments arranged by size: 

                 609     364      35
 
DraI TTT'AAA

Cuts at:      0     804    1008 
   Size:        804     204
 
DraIII CAC_nnn'GTG

Cuts at:      0     171    1008 
   Size:        171     837
 
EaeI y'GGCC_r

Cuts at:      0     417     896    1008 
   Size:        417     479     112

  Fragments arranged by size: 

                 479     417     112
 
EarI CTCTTCn'nnn_

Cuts at:      0      45     542    1008 
   Size:         45     497     466

  Fragments arranged by size: 

                 497     466      45
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     575     591     607     623    1008 
   Size:        575      16      16      16     385

  Fragments arranged by size: 

                 575     385      16      16      16
 
MfeI C'AATT_G

Cuts at:      0     847    1008 
   Size:        847     161
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     313    1008 
   Size:        313     695
 
MscI TGG'CCA

Cuts at:      0     419    1008 
   Size:        419     589
 
NcoI C'CATG_G

Cuts at:      0      44    1008 
   Size:         44     964
 
NdeI CA'TA_TG

Cuts at:      0     475     992    1002    1008 
   Size:        475     517      10       6

  Fragments arranged by size: 

                 517     475      10       6
 
NsiI A_TGCA'T

Cuts at:      0     299     469    1008 
   Size:        299     170     539

  Fragments arranged by size: 

                 539     299     170
 
NspI r_CATG'y

Cuts at:      0      20     301    1008 
   Size:         20     281     707

  Fragments arranged by size: 

                 707     281      20
 
PciI A'CATG_T

Cuts at:      0      16    1008 
   Size:         16     992
 
ScaI AGT'ACT

Cuts at:      0     650    1008 
   Size:        650     358
 
SfcI C'TryA_G

Cuts at:      0     536    1008 
   Size:        536     472
 
SphI G_CATG'C

Cuts at:      0     301    1008 
   Size:        301     707
 
SspI AAT'ATT

Cuts at:      0     410    1008 
   Size:        410     598
 
StyI C'CwwG_G

Cuts at:      0      44    1008 
   Size:         44     964
 
TatI w'GTAC_w

Cuts at:      0     486     648    1008 
   Size:        486     162     360

  Fragments arranged by size: 

                 486     360     162
 
XmnI GAAnn'nnTTC

Cuts at:      0     232    1008 
   Size:        232     776
 

 Enzymes that do cut:

     AccI   AflIII     AlfI     ApoI     BbsI    BfrBI     BmrI     BsaI
     BsgI    BspHI    BspMI  BstZ17I     BtgI     BtsI    CspCI     DraI
   DraIII     EaeI     EarI    Hin4I     MfeI     MmeI     MscI     NcoI
     NdeI     NsiI     NspI     PciI     ScaI     SfcI     SphI     SspI
     StyI     TatI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspEI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI   Bsu36I
    BtgZI     ClaI     DrdI     EagI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII
   HincII  HindIII     HpaI     KasI     KpnI     MluI     MslI   MspA1I
     NaeI     NarI   NgoMIV     NheI     NotI     NruI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SrfI     StuI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     ZraI