(Linear) (Six-base) MAPSORT of: KIBB8799.seq  Check: 4422  from: 1  to: 1014



KIBB8799.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     757    1014 
   Size:        511     246     257

  Fragments arranged by size: 

                 511     257     246
 
AlwNI CAG_nnn'CTG

Cuts at:      0     752    1014 
   Size:        752     262
 
ApoI r'AATT_y

Cuts at:      0     156     366    1014 
   Size:        156     210     648

  Fragments arranged by size: 

                 648     210     156
 
BamHI G'GATC_C

Cuts at:      0     924    1014 
   Size:        924      90
 
BclI T'GATC_A

Cuts at:      0      89    1014 
   Size:         89     925
 
BglII A'GATC_T

Cuts at:      0     703    1014 
   Size:        703     311
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      42    1014 
   Size:         42     972
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     672    1014 
   Size:        672     342
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     997    1014 
   Size:        997      17
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     461    1014 
   Size:        461     553
 
BseYI C'CCAG_C

Cuts at:      0     471    1014 
   Size:        471     543
 
BsmI GAATG_Cn'

Cuts at:      0     187     507     536    1014 
   Size:        187     320      29     478

  Fragments arranged by size: 

                 478     320     187      29
 
BsrDI GCAATG_nn'

Cuts at:      0     860    1014 
   Size:        860     154
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     502    1014 
   Size:        502     512
 
BstEII G'GTnAC_C

Cuts at:      0     755    1014 
   Size:        755     259
 
BstYI r'GATC_y

Cuts at:      0     703     924    1014 
   Size:        703     221      90

  Fragments arranged by size: 

                 703     221      90
 
DraI TTT'AAA

Cuts at:      0     902    1014 
   Size:        902     112
 
EarI CTCTTCn'nnn_

Cuts at:      0      18    1014 
   Size:         18     996
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     511     672     757    1014 
   Size:        511     161      85     257

  Fragments arranged by size: 

                 511     257     161      85
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     966    1014 
   Size:        966      48
 
HincII GTy'rAC

Cuts at:      0      72     936    1014 
   Size:         72     864      78

  Fragments arranged by size: 

                 864      78      72
 
HindIII A'AGCT_T

Cuts at:      0     385     892    1014 
   Size:        385     507     122

  Fragments arranged by size: 

                 507     385     122
 
HpaI GTT'AAC

Cuts at:      0      72    1014 
   Size:         72     942
 
MslI CAynn'nnrTG

Cuts at:      0     396     568    1014 
   Size:        396     172     446

  Fragments arranged by size: 

                 446     396     172
 
SexAI A'CCwGG_T

Cuts at:      0     751    1014 
   Size:        751     263
 
SfcI C'TryA_G

Cuts at:      0     866    1014 
   Size:        866     148
 
SmlI C'TyrA_G

Cuts at:      0     976    1014 
   Size:        976      38
 
SspI AAT'ATT

Cuts at:      0     107     169     716     828    1014 
   Size:        107      62     547     112     186

  Fragments arranged by size: 

                 547     186     112     107      62
 
TatI w'GTAC_w

Cuts at:      0     246     765    1014 
   Size:        246     519     249

  Fragments arranged by size: 

                 519     249     246
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     935    1014 
   Size:        935      79
 

 Enzymes that do cut:

     AcuI    AlwNI     ApoI    BamHI     BclI    BglII     BplI     BpmI
    BpuEI     BsaI    BseYI     BsmI    BsrDI   BstAPI   BstEII    BstYI
     DraI     EarI  Eco57MI     FalI   HincII  HindIII     HpaI     MslI
    SexAI     SfcI     SmlI     SspI     TatI     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI     BlpI Bme1580I
    BmgBI     BmrI     BmtI   Bpu10I    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
    BstBI    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI
    CspCI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FseI     FspI    FspAI    HaeII    Hin4I
     KasI     KpnI     MfeI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI