Restriction map for
pF1KB8800
(Linear) (Six-base) MAPSORT of: KIBB8800.seq Check: 5306 from: 1 to: 1026
KIBB8800.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 370 1026
Size: 370 656
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 898 1026
Size: 898 128
AflIII A'CryG_T
Cuts at: 0 341 1026
Size: 341 685
AlwNI CAG_nnn'CTG
Cuts at: 0 192 1026
Size: 192 834
BbsI GAAGACnn'nnnn_
Cuts at: 0 29 1026
Size: 29 997
BglI GCCn_nnn'nGGC
Cuts at: 0 317 406 1026
Size: 317 89 620
Fragments arranged by size:
620 317 89
BglII A'GATC_T
Cuts at: 0 682 1026
Size: 682 344
BmgBI CAC'GTC
Cuts at: 0 344 1026
Size: 344 682
BmrI ACTGGGnnnn_n'
Cuts at: 0 325 601 780 1026
Size: 325 276 179 246
Fragments arranged by size:
325 276 246 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 1026
Size: 683 343
BsaI GGTCTCn'nnnn_
Cuts at: 0 945 1026
Size: 945 81
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 1026
Size: 76 950
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 228 1026
Size: 228 798
BsiHKAI G_wGCw'C
Cuts at: 0 860 1026
Size: 860 166
Bsp1286I G_dGCh'C
Cuts at: 0 860 1026
Size: 860 166
BspHI T'CATG_A
Cuts at: 0 746 1026
Size: 746 280
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 370 1026
Size: 370 656
BsrDI GCAATG_nn'
Cuts at: 0 516 1026
Size: 516 510
BstBI TT'CG_AA
Cuts at: 0 885 1026
Size: 885 141
BstEII G'GTnAC_C
Cuts at: 0 447 541 1026
Size: 447 94 485
Fragments arranged by size:
485 447 94
BstYI r'GATC_y
Cuts at: 0 682 1026
Size: 682 344
Bsu36I CC'TnA_GG
Cuts at: 0 835 1026
Size: 835 191
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 810 1026
Size: 810 216
BtsI GCAGTG_nn'
Cuts at: 0 607 649 1026
Size: 607 42 377
Fragments arranged by size:
607 377 42
DraIII CAC_nnn'GTG
Cuts at: 0 999 1026
Size: 999 27
EaeI y'GGCC_r
Cuts at: 0 291 1026
Size: 291 735
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 683 898 1026
Size: 683 215 128
Fragments arranged by size:
683 215 128
EcoO109I rG'GnC_Cy
Cuts at: 0 839 1026
Size: 839 187
EcoRV GAT'ATC
Cuts at: 0 473 1026
Size: 473 553
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 714 1026
Size: 714 312
MscI TGG'CCA
Cuts at: 0 293 1026
Size: 293 733
MslI CAynn'nnrTG
Cuts at: 0 346 370 388 466 583 808 811 948
Size: 346 24 18 78 117 225 3 137
Cuts at: 948 1012 1026
Size: 64 14
Fragments arranged by size:
346 225 137 117 78 64 24 18
14 3
NdeI CA'TA_TG
Cuts at: 0 535 1026
Size: 535 491
NspI r_CATG'y
Cuts at: 0 111 1017 1026
Size: 111 906 9
Fragments arranged by size:
906 111 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 999 1026
Size: 999 27
PstI C_TGCA'G
Cuts at: 0 83 1026
Size: 83 943
SexAI A'CCwGG_T
Cuts at: 0 113 230 1026
Size: 113 117 796
Fragments arranged by size:
796 117 113
SfcI C'TryA_G
Cuts at: 0 79 1026
Size: 79 947
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 406 1026
Size: 406 620
StuI AGG'CCT
Cuts at: 0 409 1026
Size: 409 617
StyI C'CwwG_G
Cuts at: 0 404 933 1026
Size: 404 529 93
Fragments arranged by size:
529 404 93
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 273 456 595 1026
Size: 273 183 139 431
Fragments arranged by size:
431 273 183 139
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 25 502 1026
Size: 25 477 524
Fragments arranged by size:
524 477 25
Enzymes that do cut:
AarI AcuI AflIII AlwNI BbsI BglI BglII BmgBI
BmrI BpmI BsaI BseRI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BstBI BstEII BstYI Bsu36I BtgZI BtsI
DraIII EaeI Eco57MI EcoO109I EcoRV FalI MscI MslI
NdeI NspI PflMI PstI SexAI SfcI SfiI StuI
StyI TaqII XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbvCI BcgI BciVI BclI BfrBI BlpI Bme1580I BmtI
BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstXI BstZ17I BtgI ClaI
CspCI DraI DrdI EagI EarI EciI EcoICRI EcoNI
EcoRI FseI FspI FspAI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MspA1I NaeI
NarI NcoI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI