Restriction map for
pF1KB8806
(Linear) (Six-base) MAPSORT of: KIBB8806.seq Check: 2794 from: 1 to: 1065
KIBB8806.orf
With 182 enzymes: SgfI *
October 7, 2009 14:26 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 553 1065
Size: 553 512
Acc65I G'GTAC_C
Cuts at: 0 392 1065
Size: 392 673
AleI CACnn'nnGTG
Cuts at: 0 881 953 1065
Size: 881 72 112
Fragments arranged by size:
881 112 72
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 84 1065
Size: 84 981
AlwNI CAG_nnn'CTG
Cuts at: 0 600 1065
Size: 600 465
ApaI G_GGCC'C
Cuts at: 0 102 477 1065
Size: 102 375 588
Fragments arranged by size:
588 375 102
ApaLI G'TGCA_C
Cuts at: 0 964 1065
Size: 964 101
ApoI r'AATT_y
Cuts at: 0 529 1065
Size: 529 536
BanI G'GyrC_C
Cuts at: 0 392 1065
Size: 392 673
BanII G_rGCy'C
Cuts at: 0 102 477 1065
Size: 102 375 588
Fragments arranged by size:
588 375 102
BclI T'GATC_A
Cuts at: 0 644 734 1065
Size: 644 90 331
Fragments arranged by size:
644 331 90
BglI GCCn_nnn'nGGC
Cuts at: 0 97 427 487 1065
Size: 97 330 60 578
Fragments arranged by size:
578 330 97 60
BglII A'GATC_T
Cuts at: 0 359 1065
Size: 359 706
Bme1580I G_kGCm'C
Cuts at: 0 102 432 477 968 1065
Size: 102 330 45 491 97
Fragments arranged by size:
491 330 102 97 45
BmrI ACTGGGnnnn_n'
Cuts at: 0 303 886 1065
Size: 303 583 179
Fragments arranged by size:
583 303 179
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1065
Size: 1027 38
BsaWI w'CCGG_w
Cuts at: 0 923 1065
Size: 923 142
BseYI C'CCAG_C
Cuts at: 0 1057 1065
Size: 1057 8
BsiHKAI G_wGCw'C
Cuts at: 0 968 1065
Size: 968 97
Bsp1286I G_dGCh'C
Cuts at: 0 102 432 477 968 1065
Size: 102 330 45 491 97
Fragments arranged by size:
491 330 102 97 45
BspEI T'CCGG_A
Cuts at: 0 923 1065
Size: 923 142
BspHI T'CATG_A
Cuts at: 0 641 731 1065
Size: 641 90 334
Fragments arranged by size:
641 334 90
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 553 941 1065
Size: 248 305 388 124
Fragments arranged by size:
388 305 248 124
BsrGI T'GTAC_A
Cuts at: 0 350 1065
Size: 350 715
BstYI r'GATC_y
Cuts at: 0 320 359 1065
Size: 320 39 706
Fragments arranged by size:
706 320 39
BtgI C'CryG_G
Cuts at: 0 995 1065
Size: 995 70
BtsI GCAGTG_nn'
Cuts at: 0 876 1065
Size: 876 189
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 27 62 1065
Size: 27 35 1003
Fragments arranged by size:
1003 35 27
DraIII CAC_nnn'GTG
Cuts at: 0 414 1065
Size: 414 651
EaeI y'GGCC_r
Cuts at: 0 227 398 479 1065
Size: 227 171 81 586
Fragments arranged by size:
586 227 171 81
EarI CTCTTCn'nnn_
Cuts at: 0 250 1065
Size: 250 815
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 1065
Size: 1027 38
EcoO109I rG'GnC_Cy
Cuts at: 0 89 98 474 1065
Size: 89 9 376 591
Fragments arranged by size:
591 376 89 9
EcoRI G'AATT_C
Cuts at: 0 529 1065
Size: 529 536
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 685 717 1065
Size: 685 32 348
Fragments arranged by size:
685 348 32
HincII GTy'rAC
Cuts at: 0 888 1065
Size: 888 177
KpnI G_GTAC'C
Cuts at: 0 396 1065
Size: 396 669
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 129 1065
Size: 129 936
MscI TGG'CCA
Cuts at: 0 229 400 481 1065
Size: 229 171 81 584
Fragments arranged by size:
584 229 171 81
MslI CAynn'nnrTG
Cuts at: 0 160 815 881 953 1065
Size: 160 655 66 72 112
Fragments arranged by size:
655 160 112 72 66
PsiI TTA'TAA
Cuts at: 0 667 1065
Size: 667 398
PspOMI G'GGCC_C
Cuts at: 0 98 473 1065
Size: 98 375 592
Fragments arranged by size:
592 375 98
PstI C_TGCA'G
Cuts at: 0 550 1065
Size: 550 515
RsrII CG'GwC_CG
Cuts at: 0 434 1065
Size: 434 631
SapI GCTCTTCn'nnn_
Cuts at: 0 250 1065
Size: 250 815
SexAI A'CCwGG_T
Cuts at: 0 968 1065
Size: 968 97
SfcI C'TryA_G
Cuts at: 0 546 1065
Size: 546 519
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 97 1065
Size: 97 968
StyI C'CwwG_G
Cuts at: 0 125 1065
Size: 125 940
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 823 1065
Size: 823 242
TatI w'GTAC_w
Cuts at: 0 119 350 1065
Size: 119 231 715
Fragments arranged by size:
715 231 119
Enzymes that do cut:
AarI Acc65I AleI AlfI AlwNI ApaI ApaLI ApoI
BanI BanII BclI BglI BglII Bme1580I BmrI BpmI
BsaWI BseYI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrGI
BstYI BtgI BtsI CspCI DraIII EaeI EarI Eco57MI
EcoO109I EcoRI Hin4I HincII KpnI MmeI MscI MslI
PsiI PspOMI PstI RsrII SapI SexAI SfcI SfiI
StyI TaqII TatI
Enzymes that do not cut:
AatII AccI AclI AcuI AfeI AflII AflIII AgeI
AhdI AloI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BbeI BbsI BbvCI BcgI BciVI BfrBI BlpI
BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BsgI BsiEI BsiWI BsmI BsmBI
BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI DraI DrdI EagI
EciI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HaeII HindIII HpaI KasI MfeI MluI MspA1I NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PvuI PvuII SacI
SacII SalI SanDI SbfI ScaI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI