Restriction map for
pF1KB8812
(Linear) (Six-base) MAPSORT of: KIBB8812.seq Check: 5899 from: 1 to: 1092
KIBB8812.orf
With 182 enzymes: SgfI *
January 9, 2009 11:11 ..
Acc65I G'GTAC_C
Cuts at: 0 294 1027 1092
Size: 294 733 65
Fragments arranged by size:
733 294 65
AflIII A'CryG_T
Cuts at: 0 371 829 1092
Size: 371 458 263
Fragments arranged by size:
458 371 263
AhdI GACnn_n'nnGTC
Cuts at: 0 96 1092
Size: 96 996
AlwNI CAG_nnn'CTG
Cuts at: 0 774 1092
Size: 774 318
ApaI G_GGCC'C
Cuts at: 0 31 800 1092
Size: 31 769 292
Fragments arranged by size:
769 292 31
BamHI G'GATC_C
Cuts at: 0 129 1092
Size: 129 963
BanI G'GyrC_C
Cuts at: 0 14 294 313 431 1027 1092
Size: 14 280 19 118 596 65
Fragments arranged by size:
596 280 118 65 19 14
BanII G_rGCy'C
Cuts at: 0 31 450 509 800 809 1092
Size: 31 419 59 291 9 283
Fragments arranged by size:
419 291 283 59 31 9
BbeI G_GCGC'C
Cuts at: 0 435 1092
Size: 435 657
BbvCI CC'TCA_GC
Cuts at: 0 620 1092
Size: 620 472
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 13 47 1092
Size: 13 34 1045
Fragments arranged by size:
1045 34 13
BglI GCCn_nnn'nGGC
Cuts at: 0 440 712 1092
Size: 440 272 380
Fragments arranged by size:
440 380 272
Bme1580I G_kGCm'C
Cuts at: 0 31 254 800 1092
Size: 31 223 546 292
Fragments arranged by size:
546 292 223 31
BmrI ACTGGGnnnn_n'
Cuts at: 0 805 815 1092
Size: 805 10 277
Fragments arranged by size:
805 277 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 1092
Size: 582 510
Bpu10I CC'TnA_GC
Cuts at: 0 620 1092
Size: 620 472
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 508 1092
Size: 508 584
BsaI GGTCTCn'nnnn_
Cuts at: 0 930 1001 1051 1092
Size: 930 71 50 41
Fragments arranged by size:
930 71 50 41
BsaHI Gr'CG_yC
Cuts at: 0 97 432 1092
Size: 97 335 660
Fragments arranged by size:
660 335 97
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 892 997 1000 1092
Size: 892 105 3 92
Fragments arranged by size:
892 105 92 3
BseYI C'CCAG_C
Cuts at: 0 247 259 290 356 360 869 945 1092
Size: 247 12 31 66 4 509 76 147
Fragments arranged by size:
509 247 147 76 66 31 12 4
BsiHKAI G_wGCw'C
Cuts at: 0 199 392 509 759 790 1092
Size: 199 193 117 250 31 302
Fragments arranged by size:
302 250 199 193 117 31
BsmI GAATG_Cn'
Cuts at: 0 35 1092
Size: 35 1057
BsmBI CGTCTCn'nnnn_
Cuts at: 0 104 1092
Size: 104 988
Bsp1286I G_dGCh'C
Cuts at: 0 31 199 254 392 450 509 759 790
Size: 31 168 55 138 58 59 250 31
Cuts at: 790 800 809 1092
Size: 10 9 283
Fragments arranged by size:
283 250 168 138 59 58 55 31
31 10 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 234 491 1092
Size: 234 257 601
Fragments arranged by size:
601 257 234
BsrBI CCG'CTC
Cuts at: 0 144 1092
Size: 144 948
BsrFI r'CCGG_y
Cuts at: 0 11 1092
Size: 11 1081
BssSI C'ACGA_G
Cuts at: 0 5 1092
Size: 5 1087
BstEII G'GTnAC_C
Cuts at: 0 573 1092
Size: 573 519
BstYI r'GATC_y
Cuts at: 0 129 422 519 1092
Size: 129 293 97 573
Fragments arranged by size:
573 293 129 97
Bsu36I CC'TnA_GG
Cuts at: 0 427 1023 1092
Size: 427 596 69
Fragments arranged by size:
596 427 69
BtgI C'CryG_G
Cuts at: 0 76 182 331 710 1092
Size: 76 106 149 379 382
Fragments arranged by size:
382 379 149 106 76
DraIII CAC_nnn'GTG
Cuts at: 0 167 279 1092
Size: 167 112 813
Fragments arranged by size:
813 167 112
EaeI y'GGCC_r
Cuts at: 0 9 713 1092
Size: 9 704 379
Fragments arranged by size:
704 379 9
EarI CTCTTCn'nnn_
Cuts at: 0 187 514 870 1092
Size: 187 327 356 222
Fragments arranged by size:
356 327 222 187
EcoICRI GAG'CTC
Cuts at: 0 507 1092
Size: 507 585
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 582 1092
Size: 582 510
EcoO109I rG'GnC_Cy
Cuts at: 0 27 28 238 480 663 1050 1092
Size: 27 1 210 242 183 387 42
Fragments arranged by size:
387 242 210 183 42 27 1
HaeII r_GCGC'y
Cuts at: 0 435 1092
Size: 435 657
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 82 114 358 390 1092
Size: 82 32 244 32 702
Fragments arranged by size:
702 244 82 32 32
KasI G'GCGC_C
Cuts at: 0 431 1092
Size: 431 661
KpnI G_GTAC'C
Cuts at: 0 298 1031 1092
Size: 298 733 61
Fragments arranged by size:
733 298 61
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 890 1092
Size: 761 129 202
Fragments arranged by size:
761 202 129
MscI TGG'CCA
Cuts at: 0 715 1092
Size: 715 377
MslI CAynn'nnrTG
Cuts at: 0 75 1092
Size: 75 1017
MspA1I CmG'CkG
Cuts at: 0 360 606 624 774 1092
Size: 360 246 18 150 318
Fragments arranged by size:
360 318 246 150 18
NarI GG'CG_CC
Cuts at: 0 432 1092
Size: 432 660
NcoI C'CATG_G
Cuts at: 0 76 182 710 1092
Size: 76 106 528 382
Fragments arranged by size:
528 382 106 76
NspI r_CATG'y
Cuts at: 0 258 375 833 1092
Size: 258 117 458 259
Fragments arranged by size:
458 259 258 117
PasI CC'CwG_GG
Cuts at: 0 793 1092
Size: 793 299
PciI A'CATG_T
Cuts at: 0 371 829 1092
Size: 371 458 263
Fragments arranged by size:
458 371 263
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 570 1092
Size: 279 291 522
Fragments arranged by size:
522 291 279
PfoI T'CCnGG_A
Cuts at: 0 954 1092
Size: 954 138
PpuMI rG'GwC_Cy
Cuts at: 0 238 480 1092
Size: 238 242 612
Fragments arranged by size:
612 242 238
PspOMI G'GGCC_C
Cuts at: 0 27 796 1092
Size: 27 769 296
Fragments arranged by size:
769 296 27
PstI C_TGCA'G
Cuts at: 0 231 1092
Size: 231 861
PvuII CAG'CTG
Cuts at: 0 360 606 624 774 1092
Size: 360 246 18 150 318
Fragments arranged by size:
360 318 246 150 18
SacI G_AGCT'C
Cuts at: 0 509 1092
Size: 509 583
SanDI GG'GwC_CC
Cuts at: 0 238 1092
Size: 238 854
SapI GCTCTTCn'nnn_
Cuts at: 0 187 514 1092
Size: 187 327 578
Fragments arranged by size:
578 327 187
SfcI C'TryA_G
Cuts at: 0 227 1092
Size: 227 865
SfoI GGC'GCC
Cuts at: 0 433 1092
Size: 433 659
SmlI C'TyrA_G
Cuts at: 0 523 1092
Size: 523 569
StuI AGG'CCT
Cuts at: 0 454 1092
Size: 454 638
StyI C'CwwG_G
Cuts at: 0 76 182 710 779 800 1092
Size: 76 106 528 69 21 292
Fragments arranged by size:
528 292 106 76 69 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 151 183 1092
Size: 151 32 909
Fragments arranged by size:
909 151 32
XbaI T'CTAG_A
Cuts at: 0 748 1092
Size: 748 344
XmnI GAAnn'nnTTC
Cuts at: 0 533 1092
Size: 533 559
Enzymes that do cut:
Acc65I AflIII AhdI AlwNI ApaI BamHI BanI BanII
BbeI BbvCI BcgI BglI Bme1580I BmrI BpmI Bpu10I
BpuEI BsaI BsaHI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssSI BstEII BstYI Bsu36I
BtgI DraIII EaeI EarI EcoICRI Eco57MI EcoO109I HaeII
Hin4I KasI KpnI MmeI MscI MslI MspA1I NarI
NcoI NspI PasI PciI PflMI PfoI PpuMI PspOMI
PstI PvuII SacI SanDI SapI SfcI SfoI SmlI
StuI StyI TstI XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BsaAI BsaBI BsaWI BsaXI
BsgI BsiEI BsiWI BspEI BspHI BsrDI BsrGI BssHII
BstAPI BstBI BstXI BstZ17I BtgZI BtsI ClaI CspCI
DraI DrdI EagI EciI EcoNI EcoRI EcoRV FalI
FseI FspI FspAI HincII HindIII HpaI MfeI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SbfI ScaI SexAI SfiI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
TaqII TatI Tth111I XcmI XhoI XmaI ZraI