Restriction map for
pF1KB8817
(Linear) (Six-base) MAPSORT of: KIBB8817.seq Check: 2476 from: 1 to: 1143
KIBB8817.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AccI GT'mk_AC
Cuts at: 0 309 458 1143
Size: 309 149 685
Fragments arranged by size:
685 309 149
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 919 1143
Size: 919 224
AlwNI CAG_nnn'CTG
Cuts at: 0 22 393 891 1143
Size: 22 371 498 252
Fragments arranged by size:
498 371 252 22
ApoI r'AATT_y
Cuts at: 0 229 1143
Size: 229 914
BanI G'GyrC_C
Cuts at: 0 375 1143
Size: 375 768
BciVI GTATCCnnnnn_n'
Cuts at: 0 417 1143
Size: 417 726
BclI T'GATC_A
Cuts at: 0 224 1143
Size: 224 919
BglI GCCn_nnn'nGGC
Cuts at: 0 274 535 1143
Size: 274 261 608
Fragments arranged by size:
608 274 261
BglII A'GATC_T
Cuts at: 0 285 476 1143
Size: 285 191 667
Fragments arranged by size:
667 285 191
Bme1580I G_kGCm'C
Cuts at: 0 380 531 1143
Size: 380 151 612
Fragments arranged by size:
612 380 151
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 1108 1143
Size: 776 332 35
Fragments arranged by size:
776 332 35
Bpu10I CC'TnA_GC
Cuts at: 0 107 1143
Size: 107 1036
BsaBI GATnn'nnATC
Cuts at: 0 577 1143
Size: 577 566
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 643 1143
Size: 643 500
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 35 1143
Size: 35 1108
BsiHKAI G_wGCw'C
Cuts at: 0 949 1143
Size: 949 194
Bsp1286I G_dGCh'C
Cuts at: 0 380 531 949 1143
Size: 380 151 418 194
Fragments arranged by size:
418 380 194 151
BsrDI GCAATG_nn'
Cuts at: 0 263 1143
Size: 263 880
BsrFI r'CCGG_y
Cuts at: 0 440 1143
Size: 440 703
BstXI CCAn_nnnn'nTGG
Cuts at: 0 807 1143
Size: 807 336
BstYI r'GATC_y
Cuts at: 0 285 476 1143
Size: 285 191 667
Fragments arranged by size:
667 285 191
DraIII CAC_nnn'GTG
Cuts at: 0 305 375 1143
Size: 305 70 768
Fragments arranged by size:
768 305 70
DrdI GACnn_nn'nnGTC
Cuts at: 0 886 1143
Size: 886 257
EaeI y'GGCC_r
Cuts at: 0 548 1143
Size: 548 595
EarI CTCTTCn'nnn_
Cuts at: 0 628 1143
Size: 628 515
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 776 919 1108 1143
Size: 776 143 189 35
Fragments arranged by size:
776 189 143 35
EcoO109I rG'GnC_Cy
Cuts at: 0 355 390 888 1143
Size: 355 35 498 255
Fragments arranged by size:
498 355 255 35
FspI TGC'GCA
Cuts at: 0 244 1143
Size: 244 899
FspAI rTGC'GCAy
Cuts at: 0 244 1143
Size: 244 899
HincII GTy'rAC
Cuts at: 0 873 1143
Size: 873 270
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1143
Size: 1016 127
MscI TGG'CCA
Cuts at: 0 550 1143
Size: 550 593
MslI CAynn'nnrTG
Cuts at: 0 802 1143
Size: 802 341
MspA1I CmG'CkG
Cuts at: 0 839 1022 1032 1143
Size: 839 183 10 111
Fragments arranged by size:
839 183 111 10
NspI r_CATG'y
Cuts at: 0 243 1143
Size: 243 900
PasI CC'CwG_GG
Cuts at: 0 387 1143
Size: 387 756
PflMI CCAn_nnn'nTGG
Cuts at: 0 39 393 891 1143
Size: 39 354 498 252
Fragments arranged by size:
498 354 252 39
PfoI T'CCnGG_A
Cuts at: 0 557 584 753 890 1143
Size: 557 27 169 137 253
Fragments arranged by size:
557 253 169 137 27
PpuMI rG'GwC_Cy
Cuts at: 0 355 888 1143
Size: 355 533 255
Fragments arranged by size:
533 355 255
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 137 169 976 1008 1143
Size: 137 32 807 32 135
Fragments arranged by size:
807 137 135 32 32
PvuII CAG'CTG
Cuts at: 0 839 1143
Size: 839 304
SapI GCTCTTCn'nnn_
Cuts at: 0 628 1143
Size: 628 515
SexAI A'CCwGG_T
Cuts at: 0 371 1143
Size: 371 772
SfcI C'TryA_G
Cuts at: 0 654 993 1143
Size: 654 339 150
Fragments arranged by size:
654 339 150
SphI G_CATG'C
Cuts at: 0 243 1143
Size: 243 900
StyI C'CwwG_G
Cuts at: 0 206 1143
Size: 206 937
TatI w'GTAC_w
Cuts at: 0 399 607 905 1143
Size: 399 208 298 238
Fragments arranged by size:
399 298 238 208
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 751 804 1143
Size: 276 475 53 339
Fragments arranged by size:
475 339 276 53
Enzymes that do cut:
AccI AcuI AlwNI ApoI BanI BciVI BclI BglI
BglII Bme1580I BpmI Bpu10I BsaBI BseRI BsgI BsiHKAI
Bsp1286I BsrDI BsrFI BstXI BstYI DraIII DrdI EaeI
EarI Eco57MI EcoO109I FspI FspAI HincII MmeI MscI
MslI MspA1I NspI PasI PflMI PfoI PpuMI PsrI
PvuII SapI SexAI SfcI SphI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanII BbeI BbsI
BbvCI BcgI BfrBI BlpI BmgBI BmrI BmtI BplI
BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseYI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI EagI EciI EcoICRI
EcoNI EcoRI EcoRV FalI FseI HaeII Hin4I HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PstI
PvuI RsrII SacI SacII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XhoI XmaI XmnI ZraI