(Linear) (Six-base) MAPSORT of: KIBB8818.seq  Check: 1285  from: 1  to: 1143



KIBB8818.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 11:08  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17    1052    1143 
   Size:         17    1035      91

  Fragments arranged by size: 

                1035      91      17
 
AflII C'TTAA_G

Cuts at:      0     580    1143 
   Size:        580     563
 
AflIII A'CryG_T

Cuts at:      0     410    1143 
   Size:        410     733
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     445    1143 
   Size:        445     698
 
AlwNI CAG_nnn'CTG

Cuts at:      0      44    1143 
   Size:         44    1099
 
ApoI r'AATT_y

Cuts at:      0     258     269     760    1072    1143 
   Size:        258      11     491     312      71

  Fragments arranged by size: 

                 491     312     258      71      11
 
AseI AT'TA_AT

Cuts at:      0     467    1143 
   Size:        467     676
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1091    1124    1143 
   Size:       1091      33      19

  Fragments arranged by size: 

                1091      33      19
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1078    1143 
   Size:       1078      65
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      45    1143 
   Size:         45    1098
 
BsmI GAATG_Cn'

Cuts at:      0      71    1143 
   Size:         71    1072
 
BsrDI GCAATG_nn'

Cuts at:      0     290    1143 
   Size:        290     853
 
BsrGI T'GTAC_A

Cuts at:      0     413    1143 
   Size:        413     730
 
BstYI r'GATC_y

Cuts at:      0     966    1143 
   Size:        966     177
 
BtgI C'CryG_G

Cuts at:      0     140    1143 
   Size:        140    1003
 
BtsI GCAGTG_nn'

Cuts at:      0    1118    1143 
   Size:       1118      25
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     337     372    1143 
   Size:        337      35     771

  Fragments arranged by size: 

                 771     337      35
 
DraI TTT'AAA

Cuts at:      0    1077    1143 
   Size:       1077      66
 
DraIII CAC_nnn'GTG

Cuts at:      0     367     608    1143 
   Size:        367     241     535

  Fragments arranged by size: 

                 535     367     241
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      17    1052    1143 
   Size:         17    1035      91

  Fragments arranged by size: 

                1035      91      17
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     528     560    1143 
   Size:        528      32     583

  Fragments arranged by size: 

                 583     528      32
 
HincII GTy'rAC

Cuts at:      0     170    1143 
   Size:        170     973
 
HpaI GTT'AAC

Cuts at:      0     170    1143 
   Size:        170     973
 
MslI CAynn'nnrTG

Cuts at:      0      19     667    1143 
   Size:         19     648     476

  Fragments arranged by size: 

                 648     476      19
 
MspA1I CmG'CkG

Cuts at:      0      44    1143 
   Size:         44    1099
 
NcoI C'CATG_G

Cuts at:      0     140    1143 
   Size:        140    1003
 
NspI r_CATG'y

Cuts at:      0     414    1143 
   Size:        414     729
 
PciI A'CATG_T

Cuts at:      0     410    1143 
   Size:        410     733
 
PsiI TTA'TAA

Cuts at:      0      76    1143 
   Size:         76    1067
 
PvuII CAG'CTG

Cuts at:      0      44    1143 
   Size:         44    1099
 
ScaI AGT'ACT

Cuts at:      0    1140    1143 
   Size:       1140       3
 
SfcI C'TryA_G

Cuts at:      0     788    1143 
   Size:        788     355
 
SmlI C'TyrA_G

Cuts at:      0     580    1143 
   Size:        580     563
 
StyI C'CwwG_G

Cuts at:      0     140    1143 
   Size:        140    1003
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     461    1143 
   Size:        461     682
 
TatI w'GTAC_w

Cuts at:      0     413     810    1138    1143 
   Size:        413     397     328       5

  Fragments arranged by size: 

                 413     397     328       5
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     351     383    1143 
   Size:        351      32     760

  Fragments arranged by size: 

                 760     351      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     911    1143 
   Size:        911     232
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AlfI    AlwNI     ApoI     AseI     BaeI
    BciVI    BseRI     BsmI    BsrDI    BsrGI    BstYI     BtgI     BtsI
    CspCI     DraI   DraIII  Eco57MI    Hin4I   HincII     HpaI     MslI
   MspA1I     NcoI     NspI     PciI     PsiI    PvuII     ScaI     SfcI
     SmlI     StyI    TaqII     TatI     TstI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AleI     AloI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI     BcgI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII  HindIII
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
  Tth111I     XbaI     XcmI     XhoI     XmaI     ZraI