Restriction map for
pF1KB8819
(Linear) (Six-base) MAPSORT of: KIBB8819.seq Check: 1221 from: 1 to: 1146
KIBB8819.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1130 1146
Size: 1130 16
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 664 973 1146
Size: 664 309 173
Fragments arranged by size:
664 309 173
AflII C'TTAA_G
Cuts at: 0 297 1146
Size: 297 849
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 382 414 1146
Size: 382 32 732
Fragments arranged by size:
732 382 32
ApaI G_GGCC'C
Cuts at: 0 363 1146
Size: 363 783
ApoI r'AATT_y
Cuts at: 0 237 541 961 1146
Size: 237 304 420 185
Fragments arranged by size:
420 304 237 185
AseI AT'TA_AT
Cuts at: 0 251 1146
Size: 251 895
BanI G'GyrC_C
Cuts at: 0 903 1146
Size: 903 243
BanII G_rGCy'C
Cuts at: 0 363 1102 1146
Size: 363 739 44
Fragments arranged by size:
739 363 44
BbvCI CC'TCA_GC
Cuts at: 0 563 871 1146
Size: 563 308 275
Fragments arranged by size:
563 308 275
BglI GCCn_nnn'nGGC
Cuts at: 0 176 760 1146
Size: 176 584 386
Fragments arranged by size:
584 386 176
Bme1580I G_kGCm'C
Cuts at: 0 363 1146
Size: 363 783
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 523 1146
Size: 523 623
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 1146
Size: 329 817
Bpu10I CC'TnA_GC
Cuts at: 0 563 871 1146
Size: 563 308 275
Fragments arranged by size:
563 308 275
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 31 1146
Size: 31 1115
BsaI GGTCTCn'nnnn_
Cuts at: 0 284 1146
Size: 284 862
BsaAI yAC'GTr
Cuts at: 0 63 1146
Size: 63 1083
BsaWI w'CCGG_w
Cuts at: 0 156 1146
Size: 156 990
BsiHKAI G_wGCw'C
Cuts at: 0 822 1146
Size: 822 324
BsmBI CGTCTCn'nnnn_
Cuts at: 0 614 1146
Size: 614 532
Bsp1286I G_dGCh'C
Cuts at: 0 363 822 1102 1146
Size: 363 459 280 44
Fragments arranged by size:
459 363 280 44
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1130 1146
Size: 1130 16
BsrBI CCG'CTC
Cuts at: 0 8 55 555 1146
Size: 8 47 500 591
Fragments arranged by size:
591 500 47 8
BssSI C'ACGA_G
Cuts at: 0 745 1146
Size: 745 401
BstEII G'GTnAC_C
Cuts at: 0 159 1146
Size: 159 987
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1071 1146
Size: 1071 75
BtgI C'CryG_G
Cuts at: 0 206 1146
Size: 206 940
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 431 1146
Size: 431 715
BtsI GCAGTG_nn'
Cuts at: 0 1076 1146
Size: 1076 70
ClaI AT'CG_AT
Cuts at: 0 791 1146
Size: 791 355
DrdI GACnn_nn'nnGTC
Cuts at: 0 82 1146
Size: 82 1064
EaeI y'GGCC_r
Cuts at: 0 464 1086 1146
Size: 464 622 60
Fragments arranged by size:
622 464 60
EarI CTCTTCn'nnn_
Cuts at: 0 545 1049 1146
Size: 545 504 97
Fragments arranged by size:
545 504 97
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 256 870 1146
Size: 256 614 276
Fragments arranged by size:
614 276 256
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 329 664 973 1146
Size: 329 335 309 173
Fragments arranged by size:
335 329 309 173
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1126 1146
Size: 1126 20
EcoO109I rG'GnC_Cy
Cuts at: 0 359 360 1146
Size: 359 1 786
Fragments arranged by size:
786 359 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 412 444 1146
Size: 412 32 702
Fragments arranged by size:
702 412 32
HincII GTy'rAC
Cuts at: 0 192 1146
Size: 192 954
HpaI GTT'AAC
Cuts at: 0 192 1146
Size: 192 954
MslI CAynn'nnrTG
Cuts at: 0 494 1146
Size: 494 652
MspA1I CmG'CkG
Cuts at: 0 208 469 1146
Size: 208 261 677
Fragments arranged by size:
677 261 208
NspI r_CATG'y
Cuts at: 0 99 1117 1146
Size: 99 1018 29
Fragments arranged by size:
1018 99 29
PasI CC'CwG_GG
Cuts at: 0 174 898 1122 1146
Size: 174 724 224 24
Fragments arranged by size:
724 224 174 24
PspOMI G'GGCC_C
Cuts at: 0 359 1146
Size: 359 787
SacII CC_GC'GG
Cuts at: 0 209 1146
Size: 209 937
SapI GCTCTTCn'nnn_
Cuts at: 0 545 1146
Size: 545 601
SmlI C'TyrA_G
Cuts at: 0 46 297 1146
Size: 46 251 849
Fragments arranged by size:
849 251 46
StuI AGG'CCT
Cuts at: 0 117 1146
Size: 117 1029
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 55 1146
Size: 55 1091
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 1016 1048 1146
Size: 202 32 782 32 98
Fragments arranged by size:
782 202 98 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 526 999 1146
Size: 526 473 147
Fragments arranged by size:
526 473 147
Enzymes that do cut:
AarI AcuI AflII AloI ApaI ApoI AseI BanI
BanII BbvCI BglI Bme1580I BplI BpmI Bpu10I BpuEI
BsaI BsaAI BsaWI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BssSI BstEII BstXI BtgI BtgZI BtsI ClaI DrdI
EaeI EarI EciI Eco57MI EcoNI EcoO109I Hin4I HincII
HpaI MslI MspA1I NspI PasI PspOMI SacII SapI
SmlI StuI TaqII TstI XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflIII AgeI AhdI
AleI AlfI AlwNI ApaLI AscI AsiSI AvaI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiWI BsmI BspEI BspHI
BsrDI BsrFI BsrGI BssHII BstAPI BstBI BstYI BstZ17I
Bsu36I CspCI DraI DraIII EagI EcoICRI EcoRI EcoRV
FalI FseI FspI FspAI HaeII HindIII KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI PacI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PstI PvuI PvuII RsrII SacI SalI SanDI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI StyI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI